Combining adhesive and nonadhesive injectable hydrogels for intervertebral disc repair in an ovine discectomy model DOI Creative Commons
Christopher J. Panebianco, Caroline Constant, Jennifer Vernengo

et al.

JOR Spine, Journal Year: 2023, Volume and Issue: 6(4)

Published: Dec. 1, 2023

Abstract Background Intervertebral disc (IVD) disorders (e.g., herniation) directly contribute to back pain, which is a leading cause of global disability. Next‐generation treatments for IVD herniation need advanced preclinical testing evaluate their ability repair large defects, prevent reherniation, and limit progressive degeneration. This study tested whether experimental, injectable, nonbioactive biomaterials could slow degeneration in an ovine discectomy model. Methods Ten skeletally mature sheep (4–5.5 years) experienced partial injury with cruciate‐style annulus fibrosus (AF) defects 0.1 g nucleus pulposus (NP) removal the L1–L2, L2–L3, L3–L4 lumbar IVDs. L4–L5 IVDs were Intact controls. levels received: (1) no treatment (Injury), (2) poly (ethylene glycol) diacrylate (PEGDA), (3) genipin‐crosslinked fibrin (FibGen), (4) carboxymethylcellulose–methylcellulose (C‐MC), or (5) C‐MC FibGen (FibGen + C‐MC). Animals healed 12 weeks, then assessed using computed tomography (CT), magnetic resonance (MR) imaging, histopathology. Results All repaired retained ~90% preoperative height showed minor degenerative changes by Pfirrmann grading. repairs had similar loss grade as Injury Adhesive AF sealants (i.e., PEGDA FibGen) did not herniate, although caused local endplate (EP) inflammation. NP C‐MC) combination exhibited lower degeneration, less EP damage, severe inflammation; however, signs via biomaterial expulsion. Conclusions noninferior grade. best outcomes, may be appropriate enhancement bioactive factors cells, growth factors, miRNAs). Such appear necessary injury‐induced Application alone resulted damage inflammation, particularly IVDs, suggesting further material refinements are needed.

Language: Английский

A Redox Homeostasis Modulatory Hydrogel with GLRX3+ Extracellular Vesicles Attenuates Disc Degeneration by Suppressing Nucleus Pulposus Cell Senescence DOI
Can Liu,

Lei Fan,

Ming Guan

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(14), P. 13441 - 13460

Published: July 11, 2023

Characterized by nucleus pulposus (NP) cell senescence and extracellular matrix (ECM) degradation, disc degeneration is a common pathology for various degenerative spinal disorders. To date, effective treatments are absent. Here, we found that Glutaredoxin3 (GLRX3) an important redox-regulating molecule associated with NP degeneration. Using hypoxic preconditioning method, developed GLRX3+ mesenchymal stem cell-derived vehicles (EVs-GLRX3), which enhanced the cellular antioxidant defense, thus preventing reactive oxygen species (ROS) accumulation cascade expansion in vitro. Further, tissue-like biopolymer-based supramolecular hydrogel, was injectable, degradable, ROS-responsive, proposed to deliver EVs-GLRX3 treating rat model of degeneration, demonstrated EVs-GLRX3-loaded hydrogel attenuated mitochondrial damage, alleviated state, restored ECM deposition modulating redox homeostasis. Our findings suggested modulation homeostasis can rejuvenate attenuate

Language: Английский

Citations

39
Emerging tissue engineering strategies for annulus fibrosus therapy DOI Creative Commons
Anran Zhang, Zhangrong Cheng, Yuhang Chen

et al.

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 167, P. 1 - 15

Published: June 16, 2023

Low back pain is a major public health concern experienced by 80% of the world's population during their lifetime, which closely associated with intervertebral disc (IVD) herniation. IVD herniation manifests as nucleus pulposus (NP) protruding beyond boundaries due to disruption annulus fibrosus (AF). With deepening understanding importance AF structure in pathogenesis degeneration, numerous advanced therapeutic strategies for based on tissue engineering, cellular regeneration, and gene therapy have emerged. However, there still no consensus concerning optimal approach regeneration. In this review, we summarized field repair highlighted ideal cell types pro-differentiation targeting approaches repair, discussed prospects difficulties implant systems combining cells biomaterials guide future research directions. (AF)

Language: Английский

Citations

22
Thread-structural microneedles loaded with engineered exosomes for annulus fibrosus repair by regulating mitophagy recovery and extracellular matrix homeostasis DOI Creative Commons

Shaojun Hu,

Meng Zhu,

Hongyuan Xing

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 37, P. 1 - 13

Published: March 13, 2024

Low back pain is among the most grave public health concerns worldwide and major clinical manifestation of intervertebral disc degeneration (IVDD). The destruction annulus fibrosus (AF) primary cause IVDD. A sustainable stable treatment system for IVDD lacking because special organizational structure low nutrient supply AF. We here found that results in impaired mitochondrial function AF tissue, autophagy (mitophagy) plays a protective role this process. therefore reported thread-structural microneedle (T-MN) matching ring Based on adsorption effect laminin, our T-MN could load with bone marrow mesenchymal stem cell-derived exosomes to envelope regulating mitophagy microRNA (miRNA 378), named as

Language: Английский

Citations

6
3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function DOI Creative Commons

GaYoung Park,

Josh A. Grey,

Foteini Mourkioti

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Muscle stem cells (MuSCs) play a crucial role in skeletal muscle regeneration, residing niche that undergoes dimensional and mechanical changes throughout the regeneration process. This study investigates how 3D confinement stiffness encountered by MuSCs during later stages of regulate their function, including stemness, activation, proliferation, differentiation. An asymmetric hydrogel bilayer platform is engineered with tunable physical constraints to mimic regenerating MuSC niche. These results demonstrate increased maintains Pax7 expression, reduces activation inhibits differentiation, associated smaller nuclear size decreased H4K16ac levels, suggesting modulates both architecture epigenetic regulation. unconfined 2D environments exhibit larger nuclei higher expression compared those more confined conditions, leading progressive expansion, myogenic commitment. highlights importance cues fate regulation, acting as brake on commitment, offering novel insights into mechano-epigenetic mechanisms govern behavior regeneration.

Language: Английский

Citations

0
Self-Healing Injectable Multifunctional Hydrogels for Intervertebral Disc Disease DOI Creative Commons
Zhengrong Gu, Yi He,

Honglin Xiang

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101655 - 101655

Published: March 12, 2025

Intervertebral disc degeneration (IVDD) is increasingly prevalent in aging societies and poses a significant health challenge. Due to the limited blood supply disc, oral medications systemic treatments are often ineffective. Consequently, localized injection therapies, which deliver therapeutic agents directly degenerated have emerged as more efficient. Self-healing injectable hydrogels particularly promising due their potential for minimally invasive delivery, precise implantation, targeted drug release into hard-to-reach tissue sites, including those requiring prolonged healing. Their dynamic viscoelastic properties accurately replicate mechanical environment of natural nucleus pulposus, providing cells with an adaptive biomimetic microenvironment. This review will initially discuss anatomy pathophysiology intervertebral discs, current treatments, limitations. Subsequently, we conduct bibliometric analysis explore research hotspots trends applying hydrogel technology treat IVDD. It then features biomedical applications such drug, protein, gene engineering regenerative medicine. We construction mechanisms via physical interactions, chemical biological crosslinkers, selection biomaterials fabrication methods developing novel IVD engineering. The article concludes future perspectives on application this field.

Language: Английский

Citations

0
Design principles in mechanically adaptable biomaterials for repairing annulus fibrosus rupture: A review DOI Creative Commons
Dan Zhou, Hongmei Liu,

Zhaomin Zheng

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 31, P. 422 - 439

Published: Sept. 4, 2023

Annulus fibrosus (AF) plays a crucial role in the biomechanical loading of intervertebral disc (IVD). AF is difficult to self-heal when annulus tears develop, because has unique intricate structure and biologic milieu vivo. Tissue engineering promising for repairing rupture, but construction suitable mechanical matching devices or scaffolds still grand challenge. To deeply know varied forces involved movement native highly beneficial designing biomimetic recreate function. In this review, we overview six freedom degrees adhesion strength on tissue. Then, summarize modalities simulate related assess characteristics biomaterials. We finally outline some current advanced techniques develop mechanically adaptable biomaterials rupture repair.

Language: Английский

Citations

12
Marine biomaterials in biomedical nano/micro-systems DOI Creative Commons
Yanan Wang, Long Chen,

Yuanzheng Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 5, 2023

Abstract Marine resources in unique marine environments provide abundant, cost-effective natural biomaterials with distinct structures, compositions, and biological activities compared to terrestrial species. These marine-derived raw materials, including polysaccharides, protein components, fatty acids, minerals, etc., have shown great potential preparing, stabilizing, or modifying multifunctional nano-/micro-systems are widely applied drug delivery, theragnostic, tissue engineering, etc. This review provides a comprehensive summary of the most current biomaterial-based developed over past three years, primarily focusing on therapeutic delivery studies highlighting their cure variety diseases. Specifically, we first provided detailed introduction physicochemical characteristics biocomponents state. Furthermore, assembly processes, functionalities each building block, thorough evaluation pharmacokinetics pharmacodynamics advanced systems effects molecular pathophysiological processes were fully elucidated. Finally, list unresolved issues pivotal challenges applications, such as standardized distinction long-term biosafety vivo, feasibility scale-up, was presented. is expected serve roadmap for fundamental research facilitate rational design diverse emerging applications. Graphical

Language: Английский

Citations

12
From drugs to biomaterials: a review of emerging therapeutic strategies for intervertebral disc inflammation DOI Creative Commons

Shuhan Yang,

Shaoze Jing,

Shanxi Wang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 30, 2024

Chronic low back pain (LBP) is an increasingly prevalent issue, especially among aging populations. A major underlying cause of LBP intervertebral disc degeneration (IDD), often triggered by (IVD) inflammation. Inflammation the IVD divided into Septic and Aseptic Conservative therapy surgical treatment fail to address root IDD. Recent advances in infection inflammation range from antibiotics small-molecule drugs cellular therapies, biological agents, innovative biomaterials. This review sheds light on complex mechanisms physiological biochemical processes Furthermore, it provides overview recent research developments this area, intending identify novel therapeutic targets guide future clinical strategies for effectively treating IVD-related conditions.

Language: Английский

Citations

4
Designing hydrogel for application in spinal surgery DOI Creative Commons

Rongpeng Dong,

Shuang Zheng, Xueliang Cheng

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101536 - 101536

Published: Feb. 3, 2025

Language: Английский

Citations

0
Research progress on intervertebral disc repair strategies and mechanisms based on hydrogel DOI

Zekun Hua,

Yuyue Zhao, Meng Zhang

et al.

Journal of Biomaterials Applications, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Intervertebral disc degeneration (IDD) arises from a complex interplay of genetic, environmental, and age-related factors, culminating in spectrum low back pain (LBP) disorders that exert significant societal economic impact. The present therapeutic landscape for IDD poses formidable clinical hurdles, necessitating the exploration innovative treatment modalities. hydrogel, as biomaterial, exhibits superior biocompatibility compared to other biomaterials such bioceramics bio-metal materials. It also demonstrates mechanical properties closer those natural intervertebral discs (IVDs) favorable biodegradability conducive IVD regeneration. Therefore, it has emerged promising candidate material field regenerative medicine tissue engineering treating IDD. Hydrogels have made strides treatment. Particularly, injectable hydrogels not only provide support but enable controlled release bioactive molecules, playing crucial role mitigating inflammation promoting extracellular matrix (ECM) Furthermore, ability achieve minimally invasive implantation helps minimize damage. This article initially provides concise exposition structure function IVD, progression IDD, delineates extant interventions Subsequently, categorizes hydrogels, encapsulates recent advancements cellular therapies, delves into mechanisms through which foster Ultimately, deliberates on prospects challenges attendant hydrogel therapy

Language: Английский

Citations

0