Emerging Materials in Cultivated Meat: Engineering Sustainable Food Solutions – A Review DOI

Lingxiao Wang,

Gang Li, Xinxin Li

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Abstract As the global population grows, demand for protein‐enriched foods like meats is rising rapidly. Traditional farming practices face challenges including animal welfare, waste management, and carbon emissions, harming environment. Consequently, meat substitutes have emerged as a promising area of research an alternative to traditional livestock‐sourced meats. Cultivated meat, produced through cell culture techniques, key in cellular agriculture offers safe well‐controlled production process such future foods. The manufacturing cultivated involves several disciplines, culture, media optimization, scaffold design, advanced manufacturing, along with nutrition, taste safety topics meet consumer acceptance regulatory approvals. Although review articles addressed various aspects they tend focus on specific domains rather than comprehensive analysis this transformative technology. This highlights innovative applied findings field critical cells, materials, technologies. Furthermore, socio‐political economic impacts are explored, practical recommendations low‐cost large‐scale production. Finally, also addresses existing outlines directions development meat.

Language: Английский

Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production DOI Creative Commons

Kumiko Yamanaka,

Yuji Haraguchi, Hironobu Takahashi

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Jan. 10, 2023

Considering the amount of global resources and energy consumed, animal welfare issues associated with traditional meat production, cultured production has been proposed as a solution to these problems is attracting worldwide attention. Cultured produced by culturing/proliferating muscle cells in vitro. This process requires significant amounts culture medium, which accounts major portion cost. Furthermore, it composed nutrients derived from grains heterotrophic microorganisms fetal bovine serum (FBS), will impact sustainability future. Here, we developed novel medium containing extracted microalga cell-secreted growth factors. First, rat liver epithelial RL34 were adding Chlorella vulgaris extract (CVE) inorganic salt solution. The supernatant, factors, was used conditioned (CM). CM, CVE added nutrient source, applied primary myoblast cultures. serum-free grain-derived-nutrient-free promoted proliferation myoblasts, main cell source for beef. Our findings allow us take step toward reducing costs environmental impacts, leading an expansion market.

Language: Английский

Citations

59
Production of scaffold-free cell-based meat using cell sheet technology DOI Creative Commons
Ryu-ichiro Tanaka,

Katsuhisa Sakaguchi,

Azumi Yoshida

et al.

npj Science of Food, Journal Year: 2022, Volume and Issue: 6(1)

Published: Sept. 3, 2022

In the production of cell-based meat, it is desirable to reduce animal-derived materials as much possible meet challenges sustainability. Here, we demonstrate "cell sheet-based meat": scaffold-free meat using cell sheet technology and characterize its texture nutrients. Bovine myoblast sheets were prepared temperature-responsive culture dishes (TRCDs) 10 stacked fabricate three-dimensional tissue 1.3-2.7 mm thickness. Hardness was increased by incubation on TRCD further boiling characteristic natural meat. The wet weight percentage total protein in about half that beef. this method, large-sized items also created simply scaling up TRCD. This method promises an environment-friendly food product.

Language: Английский

Citations

43
Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells DOI Creative Commons

Gaoxiang Zhu,

Dengfeng Gao,

Linzi Li

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 9, 2023

Abstract Cultured meat production has emerged as a breakthrough technology for the global food industry with potential to reduce challenges associated environmental sustainability, public health, animal welfare, and competition between humans animals. The muscle stem cell lines currently used cultured cannot be passaged in vitro extended periods of time. Here, we develop directional differentiation system porcine pre-gastrulation epiblast cells (pgEpiSCs) stable cellular features achieve serum-free myogenic pgEpiSCs. We show that pgEpiSCs-derived skeletal progenitor fibers have typical characteristics display transcriptional during differentiation. Importantly, establish three-dimensional shaping tissue by screening plant-based edible scaffolds non-animal origin, followed generation meat. These advances provide technical approach development

Language: Английский

Citations

29
Enhancing the palatability of cultivated meat DOI
Ella G. Lambert, Christopher J. Ochs, Alexander Ward

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(9), P. 1112 - 1127

Published: March 26, 2024

Language: Английский

Citations

14
Scaffolding fundamentals and recent advances in sustainable scaffolding techniques for cultured meat development DOI Creative Commons

A.M.M. Nurul Alam,

Chan-Jin Kim,

So-Hee Kim

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 189, P. 114549 - 114549

Published: May 26, 2024

In cultured meat (CM) production, Scaffolding plays an important role by aiding cell adhesion, growth, differentiation, and alignment. The existence of fibrous microstructure in connective muscle tissues has attracted considerable interest the realm tissue engineering triggered researchers to implement scaffolding techniques. A wide array research efforts is ongoing technologies for achieving real structure on principality biomedical replace serum free CM production. Scaffolds made animal-derived biomaterials are found efficient replicating extracellular matrix (ECM), thus focus should be paid utilize animal byproducts this purpose. Proper identification utilization plant-derived biomaterial could helpful add diversified options addition derived sources reduce cost production through scaffolds. Furthermore, techniques like electrospinning, modified electrospinning 3D bioprinting focused create porous scaffolds mimic ECM form meat-like structures. This review discusses recent advances cutting edge edible related structured

Language: Английский

Citations

13
Repurposing biomedical muscle tissue engineering for cellular agriculture: challenges and opportunities DOI Creative Commons
Mohamadmahdi Samandari,

Farnoosh Saeedinejad,

Jacob Quint

et al.

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(7), P. 887 - 906

Published: March 11, 2023

Language: Английский

Citations

20
Considerations for cultivated crustacean meat: potential cell sources, potential differentiation and immortalization strategies, and lessons from crustacean and other animal models DOI Creative Commons
Lisa Musgrove, Fraser D. Russell, Tomer Ventura

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: May 11, 2024

Cultivated crustacean meat (CCM) is a means to create highly valued shrimp, lobster, and crab products directly from stem cells, thus removing the need farm or fish live animals. Conventional enterprises face increasing pressures in managing overfishing, pollution, warming climate, so CCM may provide way ensure sufficient supply as global demand for these grows. To support development of CCM, this review briefly details cell culture work date, before addressing what presently known about muscle development, particularly molecular mechanisms involved, how might relate recent on cultivated production vertebrate species. Recognizing current lack lines available establish cultures, we also consider primary sources that can be obtained non-lethally including tissues limbs which are readily released regrown, putative cells circulating hemolymph. Molecular approaches inducing myogenic differentiation immortalization reviewed. Finally, assess status tools researchers, antibodies, propose avenues address existing shortfalls order see field progress.

Language: Английский

Citations

8
3D Bioprinting of Cultured Meat: A Promising Avenue of Meat Production DOI
Xudong Guo, Dingyi Wang, Bin He

et al.

Food and Bioprocess Technology, Journal Year: 2023, Volume and Issue: 17(7), P. 1659 - 1680

Published: Sept. 2, 2023

Language: Английский

Citations

14
Microfluidic generation of bioinspired core–shell structured microfibers for cultured meat DOI
Xi Ding,

Wantao Zhuge,

Yue Zhang

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 478, P. 147467 - 147467

Published: Nov. 19, 2023

Language: Английский

Citations

14
Smart proteins as a new paradigm for meeting dietary protein sufficiency of India: a critical review on the safety and sustainability of different protein sources DOI
Raman Kumar,

Aditi Guleria,

Yogendra Padwad

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 50

Published: July 16, 2024

India, a global leader in agriculture, faces sustainability challenges feeding its population. Although primarily vegetarian population, the consumption of animal derived proteins has tremendously increased recent years. Excessive dependency on is not environmentally sustainable, necessitating identification alternative smart proteins. Smart are benign and mimic properties (dairy, egg meat) from plant proteins, microbial fermentation, insects cell culture meat (CCM) processes. This review critically evaluates technological, safety, involved production their consumer acceptance Indian context. Under current circumstances, plant-based most favorable; however, limited land availability impending climate change makes them unsustainable long run. CCM unaffordable with high input costs limiting commercialization near future. Microbial-derived could be sustainable option for future owing to higher productivity ability grow low-cost substrates. A circular economy approach integrating agri-horti waste valorization C1 substrate synthesis biomass offer economic viability. Considering use novel additives processing techniques, evaluation allergenicity, bioavailability protein products necessary before large-scale adoption.

Language: Английский

Citations

4