Published: Jan. 1, 2024
Language: Английский
ACS Nano, Journal Year: 2024, Volume and Issue: 18(18), P. 11778 - 11803
Published: April 23, 2024
Severe acute pancreatitis (AP) is a life-threatening pancreatic inflammatory disease with high mortality rate (∼40%). Existing pharmaceutical therapies in development or clinical trials showed insufficient treatment efficacy due to their single molecular therapeutic target, poor water solubility, short half-life, limited pancreas-targeting specificity, etc. Herein, acid-responsive hollow mesoporous Prussian blue nanoparticles wrapped neutrophil membranes and surface modified the N,N-dimethyl-1,3-propanediamine moiety were developed for codelivering membrane-permeable calcium chelator BAPTA-AM (BA) trypsin activity inhibitor gabexate mesylate (Ga). In AP mouse model, formulation exhibited efficient recruitment at endothelium, trans-endothelial migration, precise acinar cell targeting, resulting rapid localization higher accumulation. A low dose of (BA: 200 μg kg–1, Ga: 0.75 mg kg–1) significantly reduced pancreas function indicators close normal levels 24 h, effectively restored redox status, apoptotic proportion, blocked systemic amplified cascade, dramatic increase survival from 58.3 even 100%. Mechanistically, inhibited endoplasmic reticulum stress (IRE1/XBP1 ATF4/CHOP axis) impaired autophagy (Beclin-1/p62/LC3 axis), thereby preserving dying cells restoring cellular "health status". This provides an upstream strategy translation prospects management through synergistic ion homeostasis regulation autodigestion inhibition.
Language: Английский
Citations
20
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 20, 2025
Spinal cord injury (SCI) treatment remains a formidable challenge, as current therapeutic approaches provide only marginal relief and fail to reverse the underlying tissue damage. This study aims develop novel composite material combining enzymatic nanoparticles nerve growth factor (NGF) modulate immune microenvironment enhance SCI repair. CeMn (NP) NP-polyethylene glycol (PEG) nanozymes were synthesized via sol–gel reaction DSPE-mPEG modification. Transmission Electron Microscopy, Selected-Area Diffraction, X-ray Diffraction Photoelectron Spectroscopy confirmed their crystalline structure, mixed-valence states, redox properties. Size uniformity, biocompatibility, catalytic activity assessed hydrodynamic diameter, zeta potential, elemental analysis. The Lightgel/NGF/CeMn NP-PEG was characterized electron microscopy, compression testing, rheological analysis, NGF release kinetics, 30-day degradation studies. Both in vitro vivo experiments conducted evaluate effects of on SCI. successfully synthesized, exhibiting favorable physical At concentration 4 µg/mL, maintained cell viability demonstrated enhanced biological activity. It also showed superior mechanical properties an effective profile. Notably, significantly upregulated expression growth-associated proteins, reduced inflammatory cytokines, scavenged reactive oxygen species (ROS), promoted M2 macrophage polarization by inhibiting cyclic GMP-AMP synthase (cGAS)-stimulator interferon genes (STING) signaling pathway. In rat model, it facilitated functional recovery attenuated inflammation. shows significant promise for SCI, effectively eliminating ROS, promoting polarization, reducing pro-inflammatory supporting neuronal regeneration. These substantially motor function rats, positioning promising candidate future clinical applications.
Language: Английский
Citations
4
ACS Nano, Journal Year: 2024, Volume and Issue: 18(26), P. 16450 - 16467
Published: June 19, 2024
Nanozymes, which can selectively scavenge reactive oxygen species (ROS), have recently emerged as promising candidates for treating ischemic stroke and traumatic brain injury (TBI) in preclinical models. ROS overproduction during the early phase of these diseases leads to oxidative damage, has been a major cause mortality worldwide. However, clinical application ROS-scavenging enzymes is limited by their short vivo half-life inability cross blood-brain barrier. mimic catalytic function natural enzymes, several advantages, including cost-effectiveness, high stability, easy storage. These advantages render them superior disease diagnosis therapeutic interventions. This review highlights recent advancements nanozyme applications TBI, emphasizing potential mitigate detrimental effect overproduction, inflammation, barrier compromise. Therefore, nanozymes represent treatment modality conditions future medical practices.
Language: Английский
Citations
17
Advanced Science, Journal Year: 2024, Volume and Issue: 11(29)
Published: June 3, 2024
Abstract Exploring efficient and low‐toxicity radiosensitizers to break through the bottleneck of radiation tolerance, immunosuppression poor prognosis remains one critical developmental challenges in radiotherapy. Nanoheterojunctions, due their unique physicochemical properties, have demonstrated excellent radiosensitization effects energy deposition lifting tumor radiotherapy inhibition. Herein, they doped selenium (Se) into prussian blue (PB) construct a nano‐heterojunction (Se@PB), which could promote increase Fe 2+ /Fe 3+ ratio conversion Se high valence state with introduction. The ‐Se‐Fe electron transfer chain accelerates rate on surface nanoparticles, turn endows it X‐ray transport capability, enhances physical sensitivity. Furthermore, Se@PB induces glutathione (GSH) depletion accumulation pro‐Fenton reaction, thereby disturbs redox balance cells biochemical sensitivity As an radiosensitizer, effectively induced mitochondrial dysfunction DNA damage, promotes cell apoptosis synergistic cervical cancer This study elucidates mechanism Se‐doped nanoheterojunction from perspective biochemistry provides low‐toxic strategy
Language: Английский
Citations
14
Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122891 - 122891
Published: Oct. 12, 2024
Language: Английский
Citations
7
Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 71 - 99
Published: Feb. 13, 2025
Language: Английский
Citations
1
Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 23, 2025
Abstract Spinal cord injury (SCI) constitutes a critical occurrence that results in the disruption of both motor and sensory functions. Oxidative stress‐induced apoptosis pyroptosis have been identified as contributors to neuronal damage during secondary phase following SCI. Therefore, this study focuses on development self‐enhancing drug pair‐driven selenium (Se) nanotherapeutics, loading with 2,3,5,6‐tetramethylpyrazine (TMP) Ginsenoside Rg1 (Rg1), enhance treatment The engineered LET/TMP/Rg1@Se NPs exhibits remarkable antioxidant properties, effectively reducing oxidative by minimizing reactive oxygen species (ROS) accumulation restoring mitochondrial function. In addition their effects, nanotherapeutics demonstrates significant anti‐pyroptotic effects BV2 microglial cells modulating NLRP3/caspase‐1 pathway, leading decreased release pro‐inflammatory cytokines IL‐1β IL‐18. Moreover, inhibition inflammatory cascade response diminishes neuroinflammation‐induced promotes axonal regeneration neurons vitro. mouse model SCI, improved function regeneration, attributed pyroptosis, highlighting scientific basis for synergistic effect Se an innovative strategy effective SCI therapy.
Language: Английский
Citations
1
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(26), P. 14727 - 14746
Published: June 22, 2024
: Following spinal cord injury (SCI), autophagy plays a positive role in neuronal protection, whereas pyroptosis triggers an inflammatory response. Ginsenoside-Rh2 (GRh2), known for its neuroprotective effects, is considered promising drug. However, the exact molecular mechanisms underlying these protective effects remain unclear.
Language: Английский
Citations
4
Molecules, Journal Year: 2024, Volume and Issue: 29(14), P. 3339 - 3339
Published: July 16, 2024
Signal transduction and homeostasis are regulated by complex protein interactions in the intracellular environment. Therefore, transportation of impermeable macromolecules (nucleic acids, proteins, drugs) that control is essential for modulating cell functions therapeutic applications. However, macromolecule across membrane not easy because separates intra/extracellular environments, types molecular proteins. Cell-penetrating peptides (CPPs) expected to be carriers transport. CPPs can transport into cells through endocytosis direct translocation. The mechanism remains largely unclear owing several possibilities. In this review, we describe methods investigating CPP conformation, translocation, cargo using artificial membranes. We also investigated biomolecular living membranes via CPPs. Subsequently, show only biochemical applications but synthetic biological Finally, recent progress biomolecule nanoparticle specific tissues described from viewpoint drug delivery. This review provides opportunity discuss these two platforms.
Language: Английский
Citations
4
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139535 - 139535
Published: Jan. 5, 2025
Language: Английский
Citations
0