Biofabrication of spatially organized temporo-mandibular fibrocartilage assembloids DOI Open Access
Alexandre Dufour, Lucie Essayan, Beomjoon Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract The combination of mesenchymal stem cell (MSC) spheroids and polymeric scaffolds has been actively explored for engineering organized hyaline cartilage; however, its application to other types cartilage remains under-explored. temporo-mandibular joint (TMJ) fibrocartilage is a highly stratified tissue whose recapitulation challenging. In this study, the shape growth orientation assembloids were controlled by seeding early mature human adipose-derived MSC into with dual architecture micron-scale fibers. This results in flattened asymmetric tissues single-sided articular surface. Structurally, engineered mimicked histotypical organization observed native condylar fibrocartilage, notably featuring thick fibrous zone cells. Native-like distribution general extracellular matrix (ECM) components, including glycosaminoglycans total collagens, ECM-specific such as type I II aggrecan core protein, fibronectin, observed. Collagen organization, demonstrated polarized light microscopy scanning electron at fibril level, was also found be similar that tissue. Zonal-dependent micromechanical properties identified both tissues, although lower mechanical work provides further evidence micron-sized fiber versatile approach promising strategy biomimetic grafts TMJ reconstruction.

Language: Английский

Recent Advances in 3D Printing of Smart Scaffolds for Bone Tissue Engineering and Regeneration DOI

Xun Yuan,

Wei Zhu, Zhongyuan Yang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(34)

Published: June 11, 2024

The repair and functional reconstruction of bone defects resulting from severe trauma, surgical resection, degenerative disease, congenital malformation pose significant clinical challenges. Bone tissue engineering (BTE) holds immense potential in treating these defects, without incurring prevalent complications associated with conventional autologous or allogeneic grafts. 3D printing technology enables control over architectural structures at multiple length scales has been extensively employed to process biomimetic scaffolds for BTE. In contrast inert grafts, next-generation smart possess a remarkable ability mimic the dynamic nature native extracellular matrix (ECM), thereby facilitating regeneration. Additionally, they can generate tailored controllable therapeutic effects, such as antibacterial antitumor properties, response exogenous and/or endogenous stimuli. This review provides comprehensive assessment progress 3D-printed BTE applications. It begins an introduction physiology, followed by overview technologies utilized scaffolds. Notable advances various stimuli-responsive strategies, efficacy, applications are discussed. Finally, highlights existing challenges development implementation scaffolds, well emerging this field.

Language: Английский

Citations

60
Research Progress in Hydrogels for Cartilage Organoids DOI

Xiaolong Li,

Shihao Sheng, Guangfeng Li

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)

Published: May 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Language: Английский

Citations

29
Revolutionizing Bone Regeneration: Vascularized Bone Tissue Engineering with Advanced 3D Printing Technology DOI Creative Commons

Jiaxuan Fan,

Zichuan Ding,

Yongrui Cai

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

ABSTRACT The repair and functional reconstruction of bone defects resulting from trauma, surgical resection, degenerative diseases, congenital malformations are major clinical challenges. Bone tissue engineering has significant advantages in the treatment severe defects. Vascularized scaffolds gradually attracting attention development because their excellent biomimetic properties efficient efficiency. Three‐dimensional (3D) printing technology, which can be used to fabricate structures at different scales using a wide range materials, been production vascularized scaffolds. This review discusses research progress 3D for Angiogenesis‐osteogenesis coupling regeneration process is first introduced, followed by summary technologies, inks, bioactive factors Notably, this focuses on structural design strategies Finally, application medicine, as well challenges outlooks future development, described.

Language: Английский

Citations

2
Sustainable biofabrication: from bioprinting to AI-driven predictive methods DOI Creative Commons
Miriam Filippi,

Manuel Mekkattu,

Robert K. Katzschmann

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Language: Английский

Citations

8
Structurally Defined Cartilaginous MEW-Assembloids for Critical-size Long Bone Healing DOI
Liuqi Peng, Amit Chandrakar, Gabriella Nilsson Hall

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 319, P. 123202 - 123202

Published: Feb. 19, 2025

Language: Английский

Citations

0
4D Biofabrication of Magnetically Augmented Callus Assembloid Implants Enables Rapid Endochondral Ossification via Activation of Mechanosensitive Pathways DOI Creative Commons
Konstantinos Ioannidis, Andreas Dimopoulos, Isaak Decoene

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract The use of magnetic‐driven strategies for non‐contact manipulation engineered living modules opens up new possibilities tissue engineering. integration magnetic nanoparticles (MNPs) with cartilaginous microtissues enables model‐driven 4D bottom‐up biofabrication remotely actuated assembloids, providing unique properties to mechanoresponsive tissues, particularly skeletal constructs. However, clinical use, the long‐term effects stimulation on phenotype and in vivo functionality need further exploration. Magnetic‐driven includes both rapid processes, such as guided microtissue assembly, slower biological like extracellular matrix secretion. This work explores interplay between fields MNP‐loaded through mathematical modeling experimental approaches, investigating ECM maturation chondrogenic hypertrophy. Transcriptomic analysis reveal that activated mechanosensitive pathways catabolic driving accelerated cartilage‐to‐bone transitions via endochondral ossification, outcomes not observed non‐stimulated controls. study paves way pre‐programmed, assembloids superior bone‐forming capacity regenerating challenging bone fractures.

Language: Английский

Citations

0
Engineered stromal vascular fraction for tissue regeneration DOI Creative Commons
Jianfeng Liu, Yiwei Li, Yanan Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 13, 2025

The treatment of various tissue injuries presents significant challenges, particularly in the reconstruction large and severe defects, with conventional clinical methods often yielding suboptimal results. However, advances engineering materials have introduced new possibilities for repair. Bioactive components are commonly integrated synthetic to enhance reconstruction. Stromal vascular fraction (SVF), an adipose-derived cell cluster, has shown considerable potential regeneration due its simple efficient way obtaining richness growth factors. Therefore, this review illustrated preparation, characterization, mechanism action, applications engineered SVF repair processes, provide some references option better defect

Language: Английский

Citations

0
Osteoclast Incorporation in an In Vitro 3D Model of Endochondral Ossification DOI
Amaia Garmendia Urdalleta, Janneke Witte‐Bouma,

Nicole Kops

et al.

Tissue Engineering Part A, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

In vitro models aim to recapitulate human physiological processes, improving upon and replacing the need for animal-based models. Modeling bone formation via endochondral ossification in is a very complex process due large number of cell types involved. Most current are limited mimicking initial stages (i.e., cartilage template mineralization matrix), using single type. Chondroclasts/osteoclasts key players resorption during ossification, but their introduction into has thus far proven challenging. this study, we aimed toward new level model complexity by introducing monocyte-derived osteoclasts 3D vitro-cultured templates undergoing mineralization. Chondrogenic mineralized chondrogenic pellets were formed from pediatric marrow stromal cells cultured presence transforming growth factor-β3 (TGF-β) TGF-β/β-glycerophosphate, respectively. These have capacity form if implanted vivo. To identify suitable co-culture conditions investigate interactions, co-cultured with CD14+ monocytes an indirect (transwell) or direct setting up 14 days, osteoclastogenesis was assessed means histological stainings, osteoclast counting, gene expression analysis. Upon co-culture, achieved effective situ regions both unmineralized cartilages. Notably, vitro-generated showed ability tunnels matrix infiltrate matrix. Addition increases relevance these This will allow development robust systems study formation, disease modeling, drug discovery, further reducing animal future.

Language: Английский

Citations

0
Biofabrication of Spatially Organized Temporo‐mandibular Fibrocartilage Assembloids DOI
Alexandre Dufour, Lucie Essayan, Beomjoon Kim

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract The combination of mesenchymal stem cell (MSC) spheroids and polymeric scaffolds has been actively explored for engineering organized hyaline cartilage; however, its application to other types cartilage remains under‐explored. temporo‐mandibular joint (TMJ) fibrocartilage is a stratified tissue whose recapitulation challenging. In this study, the shape growth orientation assembloids are controlled by seeding early mature human adipose‐derived MSC into with dual architecture micron‐scale fibers. This results in flattened asymmetric tissues single‐sided articular surface. engineered mimics histotypical organization native condylar fibrocartilage, featuring thick fibrous zone cells. A native‐like distribution glycosaminoglycans, type I II collagens, aggrecan core protein, fibronectin observed. Collagen also found be similar that tissue, up fibril level. Zonal‐dependent micromechanical properties identified both tissues, although lower mechanical observed tissue. work provides further evidence micron‐sized fiber versatile approach promising strategy biomimetic grafts TMJ reconstruction.

Language: Английский

Citations

0
Harnessing the angiogenic potential of adipose-derived stromal vascular fraction cells with perfusion cell seeding DOI Creative Commons
Gregory Reid,

Giulia Cerino,

Ludovic Melly

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 1, 2025

Language: Английский

Citations

0