Ligand-based Exosome Affinity Purification (LEAP) Column Chromatography: A Tool for Clinical Applications DOI Creative Commons
Nadiar M. Mussin, Akmaral Baspakova, Kulyash R. Zhilisbayeva

et al.

West Kazakhstan Medical Journal, Journal Year: 2024, Volume and Issue: 66(4), P. 365 - 372

Published: Dec. 20, 2024

Exosomes are nano-sized extracellular vesicles that play essential roles in intercellular communication, carrying biomolecules such as proteins, lipids, and RNAs can influence physiological pathological processes. The isolation of pure exosomes is critical for both basic research clinical applications, including diagnostics therapeutics. Traditional exosome techniques, ultracentrifugation, lack specificity may yield impure samples, making the need advanced techniques evident. Ligand-based affinity purification (LEAP) column chromatography has emerged a novel method utilizes specific ligands targeting surface markers, providing highly specific, gentle, scalable approach to isolation. This mini review explores LEAP chromatography’s mechanism, benefits, potential emphasizing its g.rowing importance exosome-based therapies.

Language: Английский

Aptamer‐Coupled Polymer‐Grafted Fe3O4 Nanoparticles for Highly Efficient Isolation of Exosomes DOI Open Access

D. Huang,

Junjun Yu,

Jia Tian

et al.

Macromolecular Rapid Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Exosomes, the bioactive particles secreted by various cells, are essential in mediating cellular communication. However, their small size and interference from non‐exosome proteins present significant hurdles for rapid non‐destructive capture release. To overcome these obstacles, a promising strategy to efficiently selectively isolate exosomes mesenchymal stem cells (MSCs) is developed using CD63 aptamer‐conjugated magnetic nanoparticles (Fe 3 O 4 ‐Aptamer). The Fe first modified RAFT polymerization of N ‐(methacryloyloxy) succinimide oligoethylene glycol methacrylate, subsequently, aptamers grafted onto surface produce ‐Aptamer. These aptamer units act as “lock key” recognition with on exosomes, enabling specific binding exosomes. ‐Aptamer can conditioned medium, be easily collected an external field, facilitating facile collection multiple‐cycle reuse By introducing complementary sequence aptamer, captured rapidly released because stronger affinity between aptamers. When utilized exosome isolation, exosome‐capture release efficiency achieve up ca. 82.9% 96.1%, respectively. Thus, offers highly efficient isolation

Language: Английский

Citations

1
Tumor cell-derived engineered exosome enhances effective immunotherapy for orthotopic glioblastoma and its recurrences DOI Creative Commons
Shanshan Li,

Dongya Zhang,

Yibin Wang

et al.

Nano Today, Journal Year: 2025, Volume and Issue: 63, P. 102748 - 102748

Published: April 8, 2025

Language: Английский

Citations

0
Exosomal miRNAs in prenatal diagnosis: Recent advances DOI Creative Commons
Keqin Jin, Shuangshuang Shen,

Ruyong Shi

et al.

Medicine, Journal Year: 2024, Volume and Issue: 103(28), P. e38717 - e38717

Published: July 12, 2024

Exosomes, small membranous microvesicles released by cells, contain a range of bioactive molecules, including proteins and miRNAs, which play critical roles in intercellular communication physiological pathological processes. Current research suggests that exosomal miRNAs could serve as valuable biomarkers for prenatal diseases, offering noninvasive method early detection monitoring. Studies linking to various birth defects, fetal growth restriction, urinary tract malformations, cardiovascular system hereditary diseases like Down syndrome, were discussed. However, there are some conflicting study findings due different exosome separation methods. Here, we also discussed methods, emphasizing the importance selection based on specific purposes sample types. Further studies needed standardize isolation techniques, understand mechanisms underlying miRNA function, develop reliable diagnostic indicators. Overall, show promise potential diagnosis, but further is necessary validate their clinical utility.

Language: Английский

Citations

3
Bone Marrow Mesenchymal Stem Cell-Derived Exosomal MiR-744-5p Alleviates Obstructive Sleep Apnea-Induced Myocardial Injury by Targeting NFIX DOI Open Access
Jiarong Wang, Lili Huang, Yimin Chen

et al.

International Heart Journal, Journal Year: 2024, Volume and Issue: 65(6), P. 1144 - 1152

Published: Nov. 29, 2024

Obstructive sleep apnea (OSA) is characterized by repetitive pharyngeal collapses during sleep, which leads to intermittent hypoxia, a risk factor of OSA-related cardiovascular morbidity.

Language: Английский

Citations

1
Application of extracellular vesicles in diabetic osteoporosis DOI Creative Commons
Xiaopeng Jia,

G Zhang,

Dongmei Yu

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 10, 2024

As the population ages, occurrence of osteoporosis is becoming more common. Diabetes mellitus one factors in development osteoporosis. Compared with general population, incidence significantly higher diabetic patients. Diabetic (DOP) a metabolic bone disease characterized by abnormal tissue structure due to hyperglycemia and insulin resistance, reduced strength increased risk fractures. This complex mechanism that occurs at cellular level such as blood vessels, inflammation, resistance. Although application some drugs clinical practice can reduce DOP, fractures caused DOP still very high. Extracellular vesicles (EVs) are new communication mode between cells, which transfer miRNAs proteins from mother cells target through membrane fusion, thereby regulating function cells. In recent years, role EVs pathogenesis has been widely demonstrated. this article, we first describe changes microenvironment Second, DOP. Finally, summarize research progress challenges

Language: Английский

Citations

0
Recent advances in porous organic framework-based aptasensors for diagnosis of cancer diseases DOI
Abdelmonaim Azzouz, Lamia Hejji, Ki‐Hyun Kim

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118106 - 118106

Published: Dec. 1, 2024

Language: Английский

Citations

0
Ligand-based Exosome Affinity Purification (LEAP) Column Chromatography: A Tool for Clinical Applications DOI Creative Commons
Nadiar M. Mussin, Akmaral Baspakova, Kulyash R. Zhilisbayeva

et al.

West Kazakhstan Medical Journal, Journal Year: 2024, Volume and Issue: 66(4), P. 365 - 372

Published: Dec. 20, 2024

Exosomes are nano-sized extracellular vesicles that play essential roles in intercellular communication, carrying biomolecules such as proteins, lipids, and RNAs can influence physiological pathological processes. The isolation of pure exosomes is critical for both basic research clinical applications, including diagnostics therapeutics. Traditional exosome techniques, ultracentrifugation, lack specificity may yield impure samples, making the need advanced techniques evident. Ligand-based affinity purification (LEAP) column chromatography has emerged a novel method utilizes specific ligands targeting surface markers, providing highly specific, gentle, scalable approach to isolation. This mini review explores LEAP chromatography’s mechanism, benefits, potential emphasizing its g.rowing importance exosome-based therapies.

Language: Английский

Citations

0