Revolutionising oral organoids with artificial intelligence. DOI
Jiawei Yang, Nicholas G. Fischer, Zhou Ye

et al.

PubMed, Journal Year: 2024, Volume and Issue: 5(4), P. 372 - 389

Published: Jan. 1, 2024

The convergence of organoid technology and artificial intelligence (AI) is poised to revolutionise oral healthcare. Organoids - three-dimensional structures derived from human tissues offer invaluable insights into the complex biology diseases, allowing researchers effectively study disease mechanisms test therapeutic interventions in environments that closely mimic vivo conditions. In this review, we first present historical development organoids delve current types organoids, focusing on their use models, regeneration microbiome intervention. We then compare single-source multi-lineage assess latest progress bioprinted, vascularised neural-integrated organoids. next part highlight significant advancements AI, emphasising how AI algorithms may potentially promote for early detection diagnosis, personalised treatment, prediction drug screening. However, our main finding identification remaining challenges, such as data integration critical need rigorous validation ensure clinical reliability. Our viewpoint AI-enabled are still limited applications but, look future, potential transformation AI-integrated microbial interactions discoveries. By synthesising these components, review aims provide a comprehensive perspective state future implications role advancing healthcare improving patient outcomes.

Language: Английский

Research Progress in Hydrogels for Cartilage Organoids DOI

Xiaolong Li,

Shihao Sheng, Guangfeng Li

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)

Published: May 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Language: Английский

Citations

25
Hydrogel composite scaffold repairs knee cartilage defects: a systematic review DOI Creative Commons

Dongxu Tang,

Hao Song, Cheng Yan

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(13), P. 10337 - 10364

Published: Jan. 1, 2025

Currently, the incidence of cartilage defects in knee joints owing to different causes is increasing every year, and surgery most common treatment strategy.

Language: Английский

Citations

0
Current Status and Perspectives of Research on Polymer Hydrogels in the Treatment and Protection of Osteoarthritis DOI
Wanjun Liang, Ruey‐Jen Yang,

Lijing Qin

et al.

Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Arthritis is a degenerative disease characterized by chronic cartilage degeneration. It affects hundreds of millions people worldwide and often has serious consequences such as joint pain swelling, limited mobility, deformity. However, conventional treatments still struggle to achieve satisfactory results. Finding more effective for arthritis remains an important clinical challenge. As hydrogels have unique 3D spatial mesh structure, significant material interaction ability, adjustable mechanical properties, good biodegradability, they can provide suitable cellular or tissue microenvironment, their potential in scaffolding effect, lubrication, anti‐inflammatory drug delivery expected be potent therapeutic approach the treatment osteoarthritis. In this review, three aspects hydrogel products osteoarthritis are comprehensively summarized discussed, namely, selection gel design, exploration cross‐linking mechanisms, mechanisms therapy osteoarthritis, focus on advantages limitations applications, which point out direction development strategy innovative field, applied research, transformation.

Language: Английский

Citations

0
Ketone body 3‐hydroxybutyrate mitigates apoptosis and enhances osteogenesis in bone organoid construction via the cAMP/PKA/CREB signaling pathway DOI
S. C. Jiang, Xu Zhang,

Fanfan Chen

et al.

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(8)

Published: April 25, 2025

Abstract Bone organoids offer potential for bone regeneration and in vitro organ models. Current limitations organoid culture systems include low efficiency construction functionality, as well increased apoptosis prolonged cultures of larger sizes. The ketone body 3‐hydroxybutyrate (3HB), synthesized the liver, addresses these challenges effectively. Our findings suggest that 3HB increases intracellular calcium ion (Ca 2+ ) levels human marrow‐derived mesenchymal stem cells (hBMSCs) by activating hydroxycarboxylic acid receptor 2 (HCAR2). This activation initiates cAMP/PKA/CREB pathway, which elevates expression anti‐apoptotic genes such myeloid cell leukemia 1 (MCL1) B lymphoma 2‐related protein A1 (BCL2A1), thereby reducing apoptosis. Furthermore, this pathway boosts osteogenic proteins, including Runt‐related transcription factor (RUNX2) morphogenetic (BMP2), facilitating osteogenesis organoids. Consequently, may enhance are more mature, larger, have longer viability.

Language: Английский

Citations

0
Intelligent Manufacturing for Osteoarthritis Organoids DOI Creative Commons
Xin Lyu, Jian Wang, Jiacan Su

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

ABSTRACT Osteoarthritis (OA) is the most prevalent degenerative joint disease worldwide, imposing a substantial global burden. However, its pathogenesis remains incompletely understood, and effective treatment strategies are still lacking. Organoid technology, in which stem cells or progenitor self‐organise into miniature tissue structures under three‐dimensional (3D) culture conditions, provides promising vitro platform for simulating pathological microenvironment of OA. This approach can be employed to investigate mechanisms, carry out high‐throughput drug screening facilitate personalised therapies. review summarises structure, OA manifestations, thereby establishing context application organoid technology. It then examines components arthrosis system, specifically addressing cartilage, subchondral bone, synovium, skeletal muscle ligament organoids. Furthermore, it details various constructing organoids, including considerations cell selection, classification fabrication techniques. Notably, this introduces concept intelligent manufacturing organoids by incorporating emerging engineering technologies such as artificial intelligence (AI) process, forming an innovative software hardware cluster. Lastly, discusses challenges currently facing highlights future directions rapidly evolving field. By offering comprehensive overview state‐of‐the‐art methodologies challenges, anticipates that intelligent, automated will expedite fundamental research, discovery translational applications orthopaedic

Language: Английский

Citations

0
Peptides for Targeting Chondrogenic Induction and Cartilage Regeneration in Osteoarthritis DOI
Hsiu‐Jung Liao, Huiting Chen, Chih-Hung Chang

et al.

Cartilage, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Objects Osteoarthritis (OA) is a widespread degenerative joint condition commonly occurring in older adults. Currently, no disease-modifying drugs are available, and safety concerns associated with used traditional medications have been identified. In this review, significant portion of research field concentrated on cartilage, aiming to discover methods halt cartilage breakdown or facilitate repair. Methods Researchers mainly investigated the seeking promote its This review focuses peptide-based molecules known for their ability selectively bind growth factor cytokines components extracellular matrix. Results Chondroinductive peptides, synthetically producible, boast superior reproducibility, stability, modifiability, yield efficiency over natural biomaterials. outlines chondroinductive peptide design, molecular mechanisms, application tissue engineering also compares efficacy chondrogenesis vitro vivo. Conclusions paper, we will summarize peptides engineered regenerate by acting as scaffolds, functional molecules, both discuss additional possibilities peptides. article provides an overview our current understanding treating OA-affected explores delivery systems regeneration. These advancements may hold promise enhancing even replacing treatment methodologies.

Language: Английский

Citations

2
Future perspectives: advances in bone/cartilage organoid technology and clinical potential. DOI
Jingtao Huang,

Aikang Li,

Rongji Liang

et al.

PubMed, Journal Year: 2024, Volume and Issue: 5(4), P. 425 - 443

Published: Jan. 1, 2024

Bone and cartilage tissues are essential for movement structure, yet diseases like osteoarthritis affect millions. Traditional therapies have limitations, necessitating innovative approaches. Organoid technology, leveraging stem cells' regenerative potential, offers a novel platform disease modelling therapy. This review focuses on advancements in bone/cartilage organoid highlighting the role of cells, biomaterials, external factors development. We discuss implications these organoids medicine, research, personalised treatment strategies, presenting as promising avenue enhancing repair bone regeneration. Bone/cartilage will play greater future, promote progress biological tissue engineering.

Language: Английский

Citations

2
Bidirectionally validated in silico and in vitro formation of specific depth zone-derived chondrocyte spheroids and clusters DOI Creative Commons

Eiichiro Takada,

Hayato Laurence Mizuno, Yoshiki Takeoka

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 6, 2024

3D multicellular self-organized cluster models, e.g., organoids are promising tools for developing new therapeutic modalities including gene and cell therapies, pharmacological mechanistic screening assays. Various applications of these models have been used extensively decades, however, the mechanisms formation, maintenance, degradation not even known over in-vitro-life-time. To explore such advantageous mimicking native tissues or organs, it is necessary to understand aforementioned mechanisms. Herein, we intend clarify formation clusters. We previously demonstrated that primary chondrocytes isolated from distinct longitudinal depth zones in articular cartilage formed zone-specific spherical clusters

Language: Английский

Citations

0
Protective mechanism of Prim-O-glucosylcimifugin in the treatment of osteoarthritis: Based on lncRNA XIST regulation of Nav1.7 DOI Open Access
Changlong Fu, Yanming Lin, Qing Lin

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 181, P. 117597 - 117597

Published: Nov. 6, 2024

Language: Английский

Citations

0
Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration DOI
Wei-Cheng Chen, Dachuan Liu, Kai Lü

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development musculoskeletal organoids represents a significant breakthrough, providing novel platform studying facilitating discovery new treatments. Moreover, serve valuable complements to traditional 2D culture methods animal models, offering rich insights into biology. This review provides overview organoid technology, outlining construction processes various highlighting similarities differences. Furthermore, challenges associated systems are discussed future perspectives offered.

Language: Английский

Citations

0