Arabian Journal of Chemistry,
Journal Year:
2024,
Volume and Issue:
17(9), P. 105899 - 105899
Published: July 10, 2024
Uremic
Clearance
Granule
(UCG)
is
a
traditional
Chinese
medicines
(TCMs)
that
has
been
recognized
as
potentially
effective
treatment
for
diabetic
nephropathy
(DN).
However,
the
chemical
composition
and
hypoglycemic
ingredients
of
UCG
remain
unclear.
In
this
experiment,
magnetic
ligand
fishing
protocol
combined
with
HPLC
coupled
Fourier
transform
ion
cyclotron
resonance
mass
spectrometry
(HPLC-FT-ICR-MS)
technology
was
developed
to
screen
identify
α-Glucosidase
inhibitors
from
UCG.
First,
constituents
identified
by
HPLC-FT-ICR-MS.
Next,
coated
Fe3O4@SiO2@NH2
(Fe3O4@SiO2@NH2@α-Glucosidase)
nano
composites
synthesized.
The
essential
parameters
(concentration
Glutaraldehyde
(GA),
ratio
enzyme,
crosslinking
time
immobilization
time)
were
optimized
obtain
maximum
enzyme
activity
performance
immobilized
compared
free
enzyme.
inhibitory
activities
then
evaluated
using
autodock
4-Nitrophenyl-α-D-glucopyranoside
(pNPG)
method.
As
result,
142
compounds
comparing
retention
time,
molecular
ions
fragmentation
behaviors
reference
or
in-house
database.
20
possible
components
Molecular
docking
inhibition
studies
showed
4
compuunds
including
Limonexic
acid,
Sanggenol
A,
Glabrone,
Matrine
more
likely
stronger
than
acarbose.
conclusion,
proposed
approach,
which
highly
specific
screening
HPLC-FT-ICR-MS,
provided
powerful
platform
discovering
bioactive
multi-component
multi-target
(TCMs).
Archiv der Pharmazie,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 30, 2024
Abstract
Diabetes
is
a
serious
metabolic
disorder
affecting
individuals
of
all
age
groups
and
prevails
globally
due
to
the
failure
previous
treatments.
This
study
aims
address
most
prevalent
form
type
2
diabetes
mellitus
(T2DM)
by
reporting
on
design,
synthesis,
in
vitro
as
well
silico
evaluation
chromone‐based
thiosemicarbazones
potential
α‐glucosidase
inhibitors.
In
experiments
showed
that
tested
compounds
were
significantly
more
potent
than
standard
acarbose,
with
lead
compound
3n
exhibiting
an
IC
50
value
0.40
±
0.02
μM,
~2183‐fold
higher
acarbose
having
873.34
1.67
μM.
A
kinetic
mechanism
analysis
demonstrated
exhibited
reversible
inhibition
α‐glucosidase.
To
gain
deeper
insights,
molecular
docking,
pharmacokinetics,
dynamics
simulations
conducted
for
investigation
interactions,
orientation,
stability,
conformation
synthesized
within
active
pocket
