Biomedicine & Pharmacotherapy,
Journal Year:
2022,
Volume and Issue:
155, P. 113720 - 113720
Published: Sept. 23, 2022
Phage
Tail
Like
bacteriocins
(PTLBs)
has
been
an
area
of
interest
in
the
last
couple
years
owing
to
their
varied
application
against
multi-drug
resistant
(MDR),
anti-microbial
(AMR)
pathogens
and
evolutionary
link
with
dsDNA
virus
bacteriophages.
PTLBs
are
defective
phages
derived
from
Myoviridae
Siphoviridae
phages,
distinguished
into
R-type
(Rigid
type)
characterized
by
a
non-flexible
contractile
nanotube
resembling
phage
tails,
F-type
(Flexible
flexible
non-contractile
rod-like
structure
similar
phages.
In
this
review,
we
have
discussed
structural
association,
mechanism,
characterization
PTLBs.
Moreover,
elucidated
symbiotic
biological
function
MDR
XDR
highlighted
role
The
difficulties
that
must
be
overcome
implement
clinically
also
discussed.
It
is
imperative
these
issues
addressed
academics
future
studies
before
being
implemented
clinical
settings.
This
article
novel
its
way
as
it
will
not
only
provide
us
gateway
acts
strategy
for
scholars
mitigate
control
uprising
issue
AMR
but
promote
development
ACS Synthetic Biology,
Journal Year:
2023,
Volume and Issue:
12(3), P. 672 - 680
Published: March 3, 2023
Due
to
the
high
specificity
in
targeting
DNA
and
highly
convenient
programmability,
CRISPR-Cas-based
antimicrobials
applied
for
eliminating
specific
strains
such
as
antibiotic-resistant
bacteria
microbiome
were
gradually
developed.
However,
generation
of
escapers
makes
elimination
efficiency
far
lower
than
acceptable
rate
(10-8)
recommended
by
National
Institutes
Health.
Here,
a
systematic
study
was
carried
out
providing
insight
into
escaping
mechanisms
Escherichia
coli,
strategies
reducing
devised
accordingly.
We
first
showed
an
escape
10-5-10-3
E.
coli
MG1655
under
editing
pEcCas/pEcgRNA
established
previously.
Detailed
analysis
obtained
at
ligA
site
uncovered
that
disruption
cas9
main
cause
survivors,
notably
frequent
insertion
IS5.
Hence,
sgRNA
next
designed
target
"perpetrator"
IS5,
subsequently
killing
improved
4-fold.
Additionally,
IS-free
MDS42
also
tested
site,
∼10-fold
decrease
compared
with
MG1655,
but
still
observed
all
survivors
manifested
form
frameshifts
or
point
mutations.
Thus,
we
optimized
tool
itself
increasing
copy
number
retain
some
has
correct
sequence.
Fortunately,
rates
dropped
below
10-8
9
16
genes.
Furthermore,
λ-Red
recombination
system
added
generate
pEcCas-2.0,
100%
gene
deletion
achieved
genes
cadA,
maeB,
gntT
whereas
those
edited
low
Last,
application
pEcCas-2.0
then
expanded
B
strain
BL21(DE3)
W
ATCC9637.
This
reveals
mechanism
surviving
Cas9-mediated
death,
efficient
is
based
on
mechanism,
which
will
accelerate
further
CRISPR-Cas.
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
164, P. 114966 - 114966
Published: June 1, 2023
A
biofilm
is
a
population
of
sessile
microorganisms
that
has
distinct
organized
structure
and
characteristics
like
channels
projections.
Good
oral
hygiene
reduction
in
the
prevalence
periodontal
diseases
arise
from
minimal
accumulation
mouth,
however,
studies
focusing
on
modifying
ecology
biofilms
have
not
yet
been
consistently
effective.
The
self-produced
matrix
extracellular
polymeric
substances
greater
antibiotic
resistance
make
it
difficult
to
target
eliminate
infections,
which
lead
serious
clinical
consequences
are
often
lethal.
Therefore,
better
understanding
required
modify
order
eradicate
infection,
only
instances
disorders
but
also
terms
nosocomial
infections.
review
focuses
several
modifiers
prevent
as
well
involvement
resistance,
implants
or
in-dwelling
device
contamination,
dental
caries,
other
disorders.
It
discusses
recent
advances
nanotechnology
may
novel
strategies
for
preventing
treating
infections
caused
by
outlook
infection
control.
Poultry Science,
Journal Year:
2025,
Volume and Issue:
104(2), P. 104787 - 104787
Published: Jan. 10, 2025
Escherichia
coli
(E.
coli)
is
a
significant
pathogen
responsible
for
intestinal
infections
and
foodborne
diseases.
The
rise
of
antibiotic
resistance
poses
challenge
to
global
public
health.
Traditional
therapy
becoming
increasingly
ineffective,
highlighting
the
urgent
need
innovative
control
strategies.
This
study
explores
potential
bacteriophages
as
sustainable
alternative
traditional
antibiotics.
From
2021
2022,
total
183
non-repetitive
duck
source
fecal
samples
were
collected
from
Mianyang
City,
Sichuan
Province,
126
strains
E.
isolated.
minimum
inhibitory
concentration
(MIC)
test
showed
that
these
exhibited
high
piperacillin
(96.8%),
tetracycline
(88.9%),
chloramphenicol
(86.5%).
It
concerning
93.7%
isolates
are
classified
multidrug-resistant
(MDR),
posing
threat
existing
treatment
options.
20
isolated
soil
samples,
among
which
5
selected
further
analysis.
Bacteriophage
YP6
excellent
lytic
effects
on
MDR
strains,
especially
strain
MY104,
well
representative
serotypes
O1
MY51)
O18
MY106).
identification
member
Myoviridae
family
was
conducted
using
transmission
electron
microscopy,
it
found
have
an
optimal
infection
factor
0.1.
Bacteriophages
exhibit
thermal
pH
stability,
maintaining
survival
at
temperatures
up
60
°C
ranges
4
10.
Whole
genome
sequencing
confirmed
has
double
stranded
DNA
139,323
base
pairs
(bp),
no
or
virulence
genes
found,
indicating
low
possibility
horizontal
gene
transfer.
In
addition,
effectively
inhibits
formation
biofilm,
key
in
chronic
infections.
vivo
experiments
Galleria
mellonella
(G.
mellonella)
larvae
shown
protective
effect
against
infection.
summary,
bacteriophage
expected
become
therapeutic
agent
due
its
broad
host
range,
environmental
biofilm
inhibition
properties.
Future
research
should
optimize
preparations,
evaluate
safety
efficacy
animal
models,
establish
clinical
application
plans
field
food
safety.
Deleted Journal,
Journal Year:
2025,
Volume and Issue:
2(1)
Published: Feb. 16, 2025
Context:
The
estimated
population
of
microorganisms
inhabiting
the
gastrointestinal
tract
is
thought
to
exceed
1014,
with
a
total
weight
approximately
2
kg.
This
accumulation
microbiota
indicates
that
number
bacterial
cells
ten
times
greater
than
human
cells,
and
gene
content
exceeds
genome
by
more
hundredfold.
These
are
essential
for
maintaining
health,
having
evolved
over
millions
years.
They
influence
vital
processes
in
life,
such
as
digestion
regulation
immune
system.
gut
(GM)
can
be
viewed
dynamic
environmental
factor,
its
composition
shaped
host
genetics,
dietary
patterns,
hygiene
practices,
lifestyle
choices.
variability
positions
it
potential
therapeutic
target
cardiovascular
disease
(CVD)
myocardial
infarction
(MI).
Evidence
Acquisition:
present
study
review
factors
affecting
intestinal
relationship
heart
diseases.
To
ensure
identification
most
relevant
studies,
common
databases,
including
PubMed,
Scopus,
Web
Science,
were
searched
using
terms
"Gut
dysbiosis",
"Cardiovascular
disease",
"Probiotics",
"prebiotics".
articles
abstracts
obtained
from
search
then
reviewed.
Results:
Dysbiosis
plays
significant
role
onset
progression
atherosclerosis
CVD.
Microbiota-derived
short-chain
fatty
acids
(SCFAs)
crucial
development
blood
pressure.
Alterations
have
been
linked
dysfunctions
lipid
metabolism,
reduced
levels
high-density
lipoprotein
(HDL)
plasma
patients
GM
affect
efficiency
energy
extraction
sources,
thereby
impacting
likelihood
developing
obesity
through
inflammation
metabolism.
Conclusions:
Viruses,
Journal Year:
2025,
Volume and Issue:
17(3), P. 414 - 414
Published: March 14, 2025
The
delivery
of
biomolecules
to
target
cells
has
been
a
longstanding
challenge
in
biotechnology.
DNA
viruses
naturally
evolved
the
ability
deliver
genetic
material
and
modulate
cellular
processes.
As
such,
they
inherently
possess
requisite
characteristics
that
have
led
their
extensive
study,
engineering,
development
as
biotechnological
tools.
Here,
we
overview
application
biotechnology,
with
specific
implications
basic
research,
health,
biomanufacturing,
agriculture.
For
each
application,
review
how
an
increasing
understanding
virology
technological
methods
genetically
manipulate
enabled
advances
these
fields.
Additionally,
highlight
remaining
challenges
unlocking
full
potential
viral
technologies.
Finally,
discuss
importance
balancing
continued
progress
ethical
biosafety
considerations.
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
16
Published: March 17, 2025
Antimicrobial
resistance
(AMR)
has
become
a
major
and
escalating
global
health
threat,
undermining
the
effectiveness
of
current
antibiotic
antimicrobial
therapies.
The
rise
multidrug-resistant
bacteria
led
to
increasingly
difficult-to-treat
infections,
resulting
in
higher
morbidity,
mortality,
healthcare
costs.
Tackling
this
crisis
requires
development
novel
agents,
optimization
therapeutic
strategies,
initiatives
infection
surveillance
control.
Recent
studies
highlight
crucial
role
human
gut
microbiota
defending
against
AMR
pathogens.
A
balanced
protects
body
through
mechanisms
such
as
colonization
resistance,
positioning
it
key
ally
fight
AMR.
In
contrast,
dysbiosis
disrupts
defense,
thereby
facilitating
persistence,
colonization,
dissemination
resistant
This
review
will
explore
how
influence
drug-resistant
bacterial
its
involvement
various
types
AMR-related
potential
for
microbiota-targeted
therapies,
fecal
transplantation,
prebiotics,
probiotics,
phage
therapy.
Elucidating
interactions
between
pathogens
provide
critical
insights
developing
strategies
prevent
treat
infections.
While
previous
reviews
have
focused
on
general
impact
health,
specifically
look
at
latest
research
evolution
spread
AMR,
highlighting
strategies.
Gut Microbes,
Journal Year:
2025,
Volume and Issue:
17(1)
Published: April 14, 2025
Gut
microbiota
have
been
shown
to
influence
the
social
behaviors
of
their
hosts,
while
variations
in
host
genetics
can
affect
composition
microbiome.
Nonetheless,
degree
which
genetic
microbial
populations
impact
behavior,
as
well
any
potential
transgenerational
effects,
remains
inadequately
understood.
Utilizing
C.
elegans
a
model
organism,
we
identified
77
strains
E.
coli
from
total
3,983
mutants
that
significantly
enhanced
aggregation
behavior
through
various
neurobehavioral
pathways.
This
discovery
underscores
collaborative
regulatory
mechanism
between
and
behavior.
Notably,
observed
some
mutant
bacteria
might
via
mitochondrial
pathway.
Additionally,
modulation
has
heritable
trait
offspring.
Our
results
provide
novel
perspective
on
role
variation
may
significant
implications
for
human
studies
development
genetically
engineered
probiotics
aimed
at
enhancing
well-being
across
generations.
