Immunology,
Journal Year:
2024,
Volume and Issue:
172(2), P. 295 - 312
Published: March 7, 2024
Abstract
Hyperactivation
of
the
cyclic‐GMP‐AMP
synthase
(cGAS)–stimulator
interferon
genes
(STING)
signalling
pathway
has
been
shown
to
be
associated
with
development
a
variety
inflammatory
diseases,
and
discovery
an
inhibitor
cGAS‐STING
holds
great
promise
in
therapeutic
interventions.
Epimedium
flavonoid
(EF),
major
active
ingredient
isolated
from
medicinal
plant
Epimedium,
reported
have
good
anti‐inflammatory
activity,
but
its
exact
mechanism
action
remains
unclear.
In
present
study,
we
found
that
EF
mouse
bone
marrow‐derived
macrophages
(BMDMs),
THP‐1
(Tohoku
Hospital
Pediatrics‐1)
as
well
human
peripheral
blood
mononuclear
cells
(hPBMC)
inhibited
activation
pathway,
which
subsequently
led
decrease
expression
type
I
(IFN‐β,
CXCL10
ISG15)
pro‐inflammatory
cytokines
(IL‐6
TNF‐α).
Mechanistically,
does
not
affect
STING
oligomerization,
inhibits
formation
functional
signalosome
by
attenuating
interaction
regulatory
factor
3
(IRF3)
TANK‐binding
kinase
1
(TBK1).
Importantly,
vivo
experiments,
promising
effects
on
diseases
mediated
include
agonist
model
induced
DMXAA
stimulation,
autoimmune
disease
three
prime
repair
exonuclease
(
Trex1
)
deficiency,
non‐alcoholic
steatohepatitis
(NASH)
pathogenic
amino
acid
choline
deficiency
diet
(MCD).
To
summarize,
our
study
suggests
is
potent
potential
component
for
treatment
pathway.
World Journal of Diabetes,
Journal Year:
2024,
Volume and Issue:
15(4), P. 769 - 782
Published: April 11, 2024
BACKGROUND
Icariin
(ICA),
a
natural
flavonoid
compound
monomer,
has
multiple
pharmacological
activities.
However,
its
effect
on
bone
defect
in
the
context
of
type
1
diabetes
mellitus
(T1DM)
not
yet
been
examined.
AIM
To
explore
role
and
potential
mechanism
ICA
T1DM.
METHODS
The
effects
osteogenesis
angiogenesis
were
evaluated
by
alkaline
phosphatase
staining,
alizarin
red
S
quantitative
real-time
polymerase
chain
reaction,
Western
blot,
immunofluorescence.
Angiogenesis-related
assays
conducted
to
investigate
relationship
between
angiogenesis.
A
model
was
established
T1DM
rats.
rats
then
treated
with
or
placebo
micron-scale
computed
tomography,
histomorphometry,
histology,
sequential
fluorescent
labeling
used
evaluate
formation
area.
RESULTS
promoted
marrow
mesenchymal
stem
cell
(BMSC)
proliferation
osteogenic
differentiation.
treated-BMSCs
showed
higher
expression
levels
osteogenesis-related
markers
(alkaline
osteocalcin)
angiogenesis-related
(vascular
endothelial
growth
factor
platelet
adhesion
molecule
1)
compared
untreated
group.
also
found
induce
osteogenesis-angiogenesis
coupling
BMSCs.
In
rats,
facilitated
CD31hiEMCNhi
H-positive
capillary
formation.
Lastly,
effectively
accelerated
rate
CONCLUSION
able
accelerate
regeneration
rat
inducing
Journal of Ethnopharmacology,
Journal Year:
2024,
Volume and Issue:
330, P. 118182 - 118182
Published: April 15, 2024
Acute
Gouty
Arthritis
(AGA)
is
characterized
by
a
rapid
inflammatory
reaction
caused
the
build-up
of
monosodium
urate
(MSU)
crystals
in
tissues
surrounding
joints.
This
condition
often
associated
with
hyperuricemia,
distinguished
its
symptoms
intense
pain,
active
inflammation,
and
swelling
Traditional
approaches
AGA
management
fall
short
desired
outcomes
clinical
settings.
However,
recent
ethnopharmacological
investigations
have
been
focusing
on
potential
Herbal
Medicine
(THM)
various
forms,
exploring
their
therapeutic
impact
targets
treatment.
review
briefly
summarizes
current
pharmacological
mechanisms
THMs
–
including
ingredients,
extracts,
prescriptions
–in
treatment
AGA,
discusses
relevant
molecular
depth.
The
objective
this
study
to
offer
extensive
information
reference
point
for
exploration
targeted
using
THMs.
obtained
scientific
publications
focused
vitro
vivo
studies
anti-AGA
Medicines
(THMs)
conducted
between
2013
2023.
literature
was
collected
from
journals
electronic
databases,
PubMed,
Elsevier,
ScienceDirect,
Web
Science,
Google
Scholar.
retrieval
analysis
articles
were
guided
keywords
such
as
"acute
gouty
arthritis
Chinese
herbal
medicine,"
prescription,"
immune
cells,"
inflammation,"
NLRP3,"
miRNA,"
oxidative
stress."
We
found
that
has
large
number
targets,
highlighting
effectiveness
through
studies.
ingredients
can
mitigate
variety
influencing
macrophage
polarization,
neutrophils,
T
cells,
natural
killer
(NK)
addressing
factors
like
NLRP3
inflammasome,
signaling
pathways,
stress,
miRNA
multi-target
interactions.
properties
THMs,
components
prescriptions,
systematically
summarized
categorized
based
respective
targets.
phenolic,
flavonoid,
terpenoid
alkaloid
compounds
are
considered
key
improve
AGA.
achieve
enhanced
efficacy
interactions
multiple
which
main
target.
Nonetheless,
given
intricate
composition
traditional
medicine,
additional
research
required
unravel
underlying
alleviate
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: March 1, 2024
Abstract
Bone
defects
remain
a
significant
challenge
in
clinical
orthopedics,
but
no
targeted
medication
can
solve
these
problems.
Inspired
by
inflammatory
targeting
properties
of
macrophages,
microenvironment
bone
was
exploited
to
develop
multifunctional
nanocarrier
capable
and
promoting
regeneration.
The
avidin-modified
black
phosphorus
nanosheets
(BP-Avidin,
BP
Avi
)
were
combined
with
biotin-modified
Icaritin
(ICT-Biotin,
ICT
Bio
synthesize
(ICT)-loaded
(BP
).
then
coated
macrophage
membranes
(MMs)
obtain
MMs-camouflaged
(M@BP
Herein,
MMs
allowed
target
area,
accelerated
the
release
phosphate
ions
(PO
4
3−
when
exposed
NIR
irradiation.
PO
recruited
calcium
(Ca
2+
from
produce
Ca
3
2
,
increased
expression
osteogenesis-related
proteins.
Additionally,
M@BP
decrease
M1
polarization
pro-inflammatory
factors
promote
osteogenesis.
According
results,
provided
growth
factor
repair
material,
modulated
microenvironment,
activated
signaling
pathways
PTT
could
significantly
enhance
effects.
This
strategy
not
only
offers
solution
challenging
problem
drug-targeted
delivery
also
expands
biomedical
applications
nanocarriers.
Graphical
Immunology,
Journal Year:
2024,
Volume and Issue:
172(2), P. 295 - 312
Published: March 7, 2024
Abstract
Hyperactivation
of
the
cyclic‐GMP‐AMP
synthase
(cGAS)–stimulator
interferon
genes
(STING)
signalling
pathway
has
been
shown
to
be
associated
with
development
a
variety
inflammatory
diseases,
and
discovery
an
inhibitor
cGAS‐STING
holds
great
promise
in
therapeutic
interventions.
Epimedium
flavonoid
(EF),
major
active
ingredient
isolated
from
medicinal
plant
Epimedium,
reported
have
good
anti‐inflammatory
activity,
but
its
exact
mechanism
action
remains
unclear.
In
present
study,
we
found
that
EF
mouse
bone
marrow‐derived
macrophages
(BMDMs),
THP‐1
(Tohoku
Hospital
Pediatrics‐1)
as
well
human
peripheral
blood
mononuclear
cells
(hPBMC)
inhibited
activation
pathway,
which
subsequently
led
decrease
expression
type
I
(IFN‐β,
CXCL10
ISG15)
pro‐inflammatory
cytokines
(IL‐6
TNF‐α).
Mechanistically,
does
not
affect
STING
oligomerization,
inhibits
formation
functional
signalosome
by
attenuating
interaction
regulatory
factor
3
(IRF3)
TANK‐binding
kinase
1
(TBK1).
Importantly,
vivo
experiments,
promising
effects
on
diseases
mediated
include
agonist
model
induced
DMXAA
stimulation,
autoimmune
disease
three
prime
repair
exonuclease
(
Trex1
)
deficiency,
non‐alcoholic
steatohepatitis
(NASH)
pathogenic
amino
acid
choline
deficiency
diet
(MCD).
To
summarize,
our
study
suggests
is
potent
potential
component
for
treatment
pathway.
