Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 7, 2023
Abstract The full text of this preprint has been withdrawn by the authors while they make corrections to work. Therefore, do not wish work be cited as a reference. Questions should directed corresponding author.
Language: Английский
Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 324, P. 117829 - 117829
Published: Feb. 1, 2024
Jiao-tai-wan (JTW), a classic herbal formula of traditional Chinese medicine recorded in Han Shi Yi Tong, has been used to alleviate sleep disorders since ancient times. In modern pharmacological research, JTW adopted for treating diabetes mellitus and even exerts antidepressant effects. However, the potential mechanisms deserve further elucidation. The prevalence combined with depressive disorder (DD) is continuing increase, yet it currently under-recognized its treatment remains inadequate. present study aims explore underlying therapeutics on DD. Chronic restraint stress was db/db mice construct mouse model therapeutic effects were assessed by glucolipid metabolic indexes, behavioral tests, depression-related neurotransmitter levels. inflammatory status cell apoptosis different investigated changes cAMP/PKA/CREB pathway detected. Combining results fingerprinting molecular docking, active components screened. A cellular constructed intervention glucose corticosterone (CORT). levels neurotransmitters HT-22 cells examined, tested. Finally, activator inhibitor PKA protein reverse validation experiments. could improve impaired tolerance, lipid metabolism disorders, depression-like symptoms DD mice. Meanwhile, status, suppress microglia activation, hippocampal neuron dual might be associated activation pathway. Berberine (Ber) identified vitro experiment, up-regulate levels, Ber related as well. exert both hypoglycemic through activating signaling pathway, component, Ber, damage induced CORT via may one effective JTW.
Language: Английский
Citations
13
European Journal of Clinical Pharmacology, Journal Year: 2024, Volume and Issue: 80(7), P. 983 - 1016
Published: April 1, 2024
Language: Английский
Citations
9
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: Jan. 22, 2024
Depression, a global health problem with growing prevalence, brings serious impacts on the daily life of patients. However, antidepressants currently used in clinical are not perfectly effective, which greatly reduces compliance Berberine is natural quaternary alkaloid has been shown to have variety pharmacological effects, such as hypoglycemic, lipid-regulation, anti-cancer, antibacterial, anti-oxidation, anti-inflammatory, and antidepressant. This review summarizes evidence applications berberine treating depression elucidates mechanisms regulating neurotransmitter levels, promoting regeneration hippocampal neurons, improving hypothalamic-pituitary-adrenal axis dysfunction, anti-oxidative stress, suppressing inflammatory status order provide reference for further research application berberine.
Language: Английский
Citations
7
Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 116012 - 116012
Published: Dec. 18, 2023
Depression, a global neuropsychiatric disorder, brings serious burden to patients and society as its incidence continues rise. Berberine is one of the main compounds variety Chinese herbal medicines has been shown have multiple pharmacological effects. However, whether berberine can exert antidepressant effects in vivo vitro related mechanisms remain be explored. The chronic restraint stress (CRS) method corticosterone (CORT) were applied simulate depression-like behavior neuronal apoptosis vitro, respectively. evaluated by behavioral tests changes content monoamine neurotransmitters. Inflammatory cytokines detected immunofluorescence staining was used observe expression levels apoptosis-related proteins. RT-qPCR Western blot examine mRNA protein (or phosphorylation) biomarkers PI3K/AKT/CREB/BDNF signaling pathways. Behavioral neurotransmitters proved that could effectively ameliorate symptoms CRS mice. Meanwhile, results ELISA showed alleviate inflammatory status reduce cell vitro. Moreover, pathway induced or CORT mouse hippocampus HT-22 cells significantly reversed berberine. Our current study suggested vivo, which may associated with pathway.
Language: Английский
Citations
12
Chinese Herbal Medicines, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
Frontiers in Behavioral Neuroscience, Journal Year: 2023, Volume and Issue: 17
Published: Aug. 28, 2023
Background Ascending clinical evidence supports that electroacupuncture (EA) is effective in treating post-ischemic depression (PID), but little known about how it works at the cellular level. Astrocytes are exquisitely sensitive to their extracellular environment, and under stressful conditions, they may experience aberrant structural remodeling can potentially cause neuroplastic disturbances contribute subsequent changes mood or behavior. Objectives This study aimed investigate effect of EA on behavioral deficits associated with PID mice verify hypothesis astrocytic morphology be involved this impact. Methods We established a animal model induced by transient bilateral common carotid artery occlusion (BCCAO, 20 min) chronic restraint stress (CRS, 21 days). treatment (GV20 + ST36) was performed for 3 weeks, from Monday Friday each week. Depressive- anxiety-like behaviors sociability were evaluated using SPT, FST, EPM, SIT. Immunohistochemistry combined Sholl cell morphological analysis utilized assess process GFAP+ astrocytes mood-related regions. The potential relationship between output detected correlation analysis. Results Behavioral assays demonstrated an overall reduction deficits, as measured Z-score. analyses revealed prevented decline complexity prefrontal cortex (PFC) CA1 region hippocampus, where displayed evident deramification atrophy branches. Eventually, showed there emotionality changes. Conclusion Our findings imply prevents both abnormalities model. strong Z-scores observed confirms notion weakening processes play crucial role depressive symptoms, could target PID.
Language: Английский
Citations
4
Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 19(6), P. 1351 - 1359
Published: Sept. 22, 2023
Abstract JOURNAL/nrgr/04.03/01300535-202406000-00040/inline-graphic1/v/2023-10-30T152229Z/r/image-tiff Tibetan singing bowls emit low-frequency sounds and produce perceptible harmonic tones vibrations through manual tapping. The the have been shown to enhance relaxation reduce anxiety. However, underlying mechanism remains unclear. In this study, we used chronic restraint stress or sleep deprivation establish mouse models of anxiety that exhibit anxiety-like behaviors. We then supplied treatment with in a bottomless cage placed on top cushion. found unlike humans, combination did not improve behaviors mice, while individual vibration components did. Additionally, increased level N-methyl-D-aspartate receptor 1 somatosensory cortex prefrontal decreased γ-aminobutyric acid A (GABA) α subtype, reduced CaMKII cortex, number GABAergic interneurons. At same time, electrophysiological tests showed significantly abnormal gamma oscillation peak frequency medial caused by pressure deprivation. Results from study indicate can alleviate reducing molecular events cortex.
Language: Английский
Citations
4
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0303189 - e0303189
Published: May 20, 2024
Objectives To establish a rat model that accurately replicates the clinical characteristics of male infertility (MI) with Liver Depression and Kidney Deficiency (LD & KD) investigate pathogenesis. Methods After subjecting rats to chronic restraint stress (CRS) adenine treatment, series tests were conducted, including ethological assessments, evaluations reproductive characteristics, measurements biochemical parameters, histopathological examinations, analyses urinary metabolites. Additionally, bioinformatics predictions performed for comprehensive analysis. Results Compared control, exhibited significant manifestations MI LD KD, reduced responsiveness, diminished frequency capturing estrous female rats, absence mounting behavior. kidney coefficient increased markedly, while coefficients testis epididymis decreased significantly. Sperm counts viabilities notably, accompanied by an increase in sperm abnormalities. Dysregulation hormone levels serum was observed, upregulation proinflammatory cytokines expressions liver kidney, as well exacerbated oxidative penile corpus cavernosum testis. The seminiferous tubules loose arrangement, loss germ cells, infiltration inflammatory cells. Furthermore, utilizing metabolomics analysis, 5 key biomarkers 2 crucial targets most closely linked revealed. Conclusion study successfully established clinically relevant animal KD. It elucidates pathogenesis condition, identifies targets, provides robust scientific foundation prediction, diagnosis, treatment
Language: Английский
Citations
1
Natural Product Communications, Journal Year: 2024, Volume and Issue: 19(8)
Published: Aug. 1, 2024
Objectives The aim is to discover α-glucosidase/acetylcholinesterase inhibitors as lead compounds from a traditional herbal prescription of Qi-Li-Qiang-Xin capsule (QLQX). Methods A novel strategy combining time-based fractionation, LC-QTOF-MS, enzymatic activity assay, molecular docking and component-target association analysis was performed QLQX. Time-based fractionation combined with assay used find the distribution period active in prescription. LC-QTOF-MS analyze structure compounds. Molecular applied explore interaction between targets. Results According components QLQX for acetylcholinesterase were primarily concentrated highly polar region, whereas α-glucosidase predominantly found moderately area. total 33 identified by comparing chemical reference substances. Dihydrotanshinone Ⅰ (16.98 µM), hydroxysafflor yellow (84.57 salvianolic acid (76.62 µM) cryptotanshinone (112.68 Similarly, rosmarinic (62.29 isochlorogenic (17.95 4,5-dicaffeoylquinic (117.93 danshensu (207.88 (1.31 3,4-dicaffeoylquinic (91.71 formononetin (67.26 ginsenoside Rd (3.43 Rb1 (26.37 F1 (18.79 discovered inhibitors. Notably, inhibited both α-glucosidase. results indicated that hydrogen bonds, hydrophobic interactions Pi-Pi T-shaped crucial inhibiting acetylcholinesterase. Meanwhile, stacked significant suppressing Conclusion contains numerous inhibitors, demonstrating its potential therapeutic benefits Alzheimer's disease diabetes.
Language: Английский
Citations
1
Phytomedicine, Journal Year: 2024, Volume and Issue: 134, P. 155954 - 155954
Published: Aug. 13, 2024
Cognitive impairment (CI) is now well-accepted as a complication and comorbidity of diabetes mellitus (DM), becoming serious medical social problem. Jiao-tai-wan (JTW), one noted traditional Chinese medicine (TCM), showed dual therapeutic effects on DM CI. Nevertheless, the potential mechanism unclear.
Language: Английский
Citations
1