Protective Effects of a Polyherbal Mixture on Intestinal Injury via the NF-κB Signaling Pathway and Gut Microbiota Modulation in Hyperuricemic Mice

Foods, Journal Year: 2025, Volume and Issue: 14(7), P. 1118 - 1118

Published: March 24, 2025

This study investigated the protective effects of a polyherbal tea (PHT) on intestinal injury in hyperuricemia (HUA) mice and underlying mechanisms. PHT was orally administered to for 49 days, while potassium oxonate hypoxanthine were 7 days after administration continued 42 cause HUA. Treatment with significantly reduced serum uric acid blood urea nitrogen levels HUA mice. It also inhibited liver xanthine oxidase activity promoted excretion through upregulation transporters GLUT9 ABCG2. Intestinal barrier integrity reinforced, as evidenced by restoration villous structure, reduction edema, tight junction proteins (occludin, ZO-1) mucin (MUC2). Moreover, suppressed LPS NF-κB pathway, leading TNF-α IL-6 gut. Gut microbiota analysis revealed reversed dysbiosis, enriching beneficial bacteria like Duncaniella sp. Heminiphilus faecis. By UPLC-MS analysis, 154 compounds persisted gut, suggesting that these are likely modulate both function gut microbiota. These findings suggest this may have potential functional food prevention hyperuricemia.

Language: Английский

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Effects of hydroxypropyl starch on intestinal health and transcriptome of geese

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 10, 2025

Abstract In recent years, gout resulting from uric acid metabolism disorders has led to significant economic losses in goose production. The intestine is a vital organ crucial for metabolism. Hydroxypropyl starch (HPS) resistant modified natural starch, which can enhance intestinal health as dietary ingredient fiber. this study, 240 30-day-old Yangzhou geese with similar body weights were divided into three groups: control group (CG) received basal diet + 5% corn starch; the hydroxypropyl and sodium urate (SU) given starch. experiment lasted 21 days, SU was administered 30 mg of per day during last 4 days study. results indicated that level HPS 56.6 µmol/L, significantly lower than CG (70.8 µmol/L) (129.7 µmol/L). morphological findings revealed ileum exhibited varying degrees damage, while maintained complete structure. villus height ratio crypt depth higher compared those groups, group. A total 1462 differentially expressed genes (DEGs) identified at transcriptome level. GO KEGG functional enrichment analyses DEGs enriched Brush border membrane, border, PPAR signaling pathway, PI3K-Akt other related processes. Subsequent analysis up-regulated expression associated function (such SLC5A12 SLC5A8), structure (including NR5A2, IPMK), (PDZK1). accuracy reliability sequencing data confirmed by RT-qPCR. we systematically demonstrated improve morphology reduce serum levels, emphasizing its potential supplement geese.

Language: Английский

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Alginate ameliorates hyperuricemia in mice by restoring hyperuricemia-induced renal and intestinal dysfunctions

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143422 - 143422

Published: April 1, 2025

Alginate, a bioactive polysaccharide fermentable by gut microbiota, has been shown to effectively reduce serum uric acid levels. However, its mechanisms and the role of microbiota remain unclear. In this study, we explored effects alginate with two different molecular weights on hyperuricemia mice. Both alginates exhibited potent hypouricemic through ABCG2 transporter upregulation, ameliorating hyperuricemia-induced renal intestinal dysfunctions, low-molecular-weight demonstrating enhanced bioavailability microbial biodegradation superior therapeutic efficacy in management. Additionally, found that alleviates dysbiosis induced enriching potentially beneficial bacteria. These include Limosilactobacillus Lactobacillus, which show significant negative correlation bacteria might regulate precursors during purine metabolism, thereby reducing accumulation. summary, study reveals protective damage mice highlights crucial microbiota. It provides valuable insights into mediate alginate.

Language: Английский

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Sodium butyrate promotes synthesis of testosterone and meiosis of hyperuricemic male mice

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 28, 2025

Hyperuricemia (HUA) impaires spermatogenesis. This study was carried out, aiming to determine whether butyric acid (NaB) avoids the HUA-induced decline of sperm quality HUA mice were developed through intra-peritoneal injection potassium oxalate combined with intragastric uric (UA) and by tube feeding 300 mg·kg-1·d-1NaB. The effect NaB on reproduction male determined measuring count, motility testosterone content. In addition, TM3 GC-2 cells treated a solution containing 30 mg/dl UA 1mM NaB. effects evaluated expression level genes involving in LH/cAMP/PKA signaling pathway meiosis, that encoding OPRL1 receptor protein. Results showed improved motility, synthesis, impaired spermatocyte meiosis via HUA. vitro analysis activated cells, promoted synthesis testosterone, up-regulated content pain-sensitive peptide (OPRL1) surface meiosis. also utilization ATP cells. We illustrated close relationship between spermatogenesis defects. NaB-promoted functioning testis may aid improving quality.

Language: Английский

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Evaluation of purine-nucleoside degrading ability and in vivo uric acid lowering of Streptococcus thermophilus IDCC 2201, a novel antiuricemia strain

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(2), P. e0293378 - e0293378

Published: Feb. 22, 2024

This study evaluated 15 lactic acid bacteria with a focus on their ability to degrade inosine and hypo-xanthine—which are the intermediates in purine metabolism—for management of hyperuricemia gout. After preliminary screening based HPLC, Lactiplantibacillus plantarum CR1 pentosus GZ1 were found have highest nucleoside degrading rates, they therefore selected for further characterization. S . thermophilus IDCC 2201, which possessed hpt gene encoding hypoxanthine-guanine phosphoribosyltransferase (HGPRT) exhibited degradation, was also These three strains examined terms probiotic effect lowering serum uric Sprague-Dawley (SD) rat model potassium oxonate (PO)-induced hyperuricemia. Among these strains, level most reduced by 2201 (p < 0.05). Further, analysis microbiome showed that administration thermophlilus led significant difference gut microbiota composition compared group administered PO-induced Moreover, intestinal short-chain fatty acids (SCFAs) be significantly increased. Altogether, results this work indicate lowers levels purine-nucleosides restores flora SCFAs, ultimately suggesting is promising candidate use as an adjuvant treatment patients

Language: Английский

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Gut-Microbiota-Derived Butyric Acid Overload Contributes to Ileal Mucosal Barrier Damage in Late Phase of Chronic Unpredictable Mild Stress Mice

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12998 - 12998

Published: Dec. 3, 2024

Intestinal mucosal barrier damage is regarded as the critical factor through which chronic unpredictable mild stress (CUMS) leads to a variety of physical and mental health problems. However, exact mechanism by CUMS induces intestinal unclear. In this study, 14, 28, 42 d model mice were established. The indicators related ileal (IMBD), composition microbiota its amino acid (AA) short-chain fatty (SCFA) metabolic functions, free (FAA) SCFA levels in lumen measured before after each period. correlations between them are analyzed investigate how male C57BL/6 mice. With progression CUMS, butyric (BA) decreased (14 28 d) then increased (42 d), IMBD progressively increased. late stage degree most severe positively correlated with significantly BA (

Language: Английский

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Commensal Enterococcus faecalis W5 ameliorates hyperuricemia and maintains the epithelial barrier in a hyperuricemia mouse model

Journal of Digestive Diseases, Journal Year: 2023, Volume and Issue: 25(1), P. 44 - 60

Published: Dec. 21, 2023

Objective The intestine is responsible for approximately one‐third of uric acid (UA) excretion. effect commensal Enterococcus faecalis ( E. ), one the most colonized bacteria in gut, on UA excretion remains to be investigated. aim this study was evaluate metabolism and gut microbiota. Methods 16S rRNA gene sequencing used examine species mouse fecal content. strain isolated from feces identified W5. hyperuricemia (HUA) animal model established with yeast‐rich forage 250 mg·kg −1 ·day potassium oxonate. Oral administration W5 given 20 days, serving as Efa group. Results Disrupted intestinal barrier, activated proinflammatory response low were found HUA mice. After treatment, barrier restored serum level decreased. Additionally, levels elevated, urate transporter ABCG2 purine upregulated. Moreover, short‐chain fatty increased, inflammation ameliorated. Conclusions Commensal ameliorated through reversing impaired promoting secretion by regulating expression, decreasing synthesis metabolism. results may provide potential developing treatments intestine.

Language: Английский

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Lactiplantibacillus plantarum 06CC2 Enhanced the Expression of Intestinal Uric Acid Excretion Transporter in Mice

Nutrients, Journal Year: 2024, Volume and Issue: 16(17), P. 3042 - 3042

Published: Sept. 9, 2024

ATP-binding cassette transporter subfamily G member 2 (ABCG2) is responsible for the excretion of foreign substances, such as uric acid (UA) and indoxyl sulfate (IS), from body. Given importance increased ABCG2 expression in UA excretion, we investigated enhancement intestinal using

Language: Английский

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The Independent Value of Neutrophil to Lymphocyte Ratio in Gouty Arthritis: A Narrative Review

Journal of Inflammation Research, Journal Year: 2023, Volume and Issue: Volume 16, P. 4593 - 4601

Published: Oct. 1, 2023

Abstract: Since the incidence of gouty arthritis (GA) exhibits yearly increases, accurate assessment and early treatment have significant values for improving disease conditions monitoring prognosis. Neutrophil to lymphocyte ratio (NLR) is a common indicator in blood routine, which has characteristics easy access low cost. In recent years, NLR been proven be an effective guiding diagnosis, treatment, prognosis various diseases. Moreover, varying degrees relationship with inflammatory biomarkers, can affect reflect response body. This paper reviews independent value GA its underlying molecular pathological mechanisms, intending contribute further application NLR. Keywords: neutrophil ratio, arthritis, response, neutrophils, activity

Language: Английский

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Mitigating lead-induced osteoporosis: The role of butyrate in gut-bone axis restoration

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 283, P. 116943 - 116943

Published: Aug. 30, 2024

Lead (Pb) is an environmentally widespread bone toxic pollutant, contributes to the development of osteoporosis. Butyric acid, mainly produced by fermentation indigestible dietary fiber gut microbiota, plays a pivotal role in maintenance homeostasis. However, effects butyric acids on Pb induced osteoporosis have not yet been elucidated. In this study, our results showed that exposure was negatively related abundance Pb-exposed population and mice. caused microbiota disorders, resulting decline acid-producing bacteria, such as Butyrivibrio_crossotus, Clostridium_sp._JN9, butyrate-producing enzymes through acetyl-CoA pathway. Moreover, from NHANES data suggested intake butyrate associated with reduced risk lead-burdened populations, particularly among men or participants aged 18–60 years. addition, supplementation mice chronic improved microarchitectures, repaired intestinal damage, upregulated proportion Treg cells. Taken together, these demonstrated disturbs gut-bone axis, which can be restored acid supplement. Our suggest possible therapeutic strategy for lead-induced toxicity.

Language: Английский

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