Role and therapeutic targets of P2X7 receptors in neurodegenerative diseases

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 2, 2024

The P2X7 receptor (P2X7R), a non-selective cation channel modulated by adenosine triphosphate (ATP), localizes to microglia, astrocytes, oligodendrocytes, and neurons in the central nervous system, with most incredible abundance microglia. P2X7R partake various signaling pathways, engaging immune response, release of neurotransmitters, oxidative stress, cell division, programmed death. When neurodegenerative diseases result neuronal apoptosis necrosis, ATP activates P2X7R. This activation induces biologically active molecules such as pro-inflammatory cytokines, chemokines, proteases, reactive oxygen species, excitotoxic glutamate/ATP. Subsequently, this leads neuroinflammation, which exacerbates involvement. is essential development diseases. implies that it has potential drug target could be treated using antagonists are able cross blood-brain barrier. review will comprehensively objectively discuss recent research breakthroughs on genes, their structural features, functional properties, roles possible therapies.

Language: Английский

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Design and Self-Assembly of Peptide-Copolymer Conjugates into Nanoparticle Hydrogel for Wound Healing in Diabetes

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2487 - 2506

Published: March 1, 2024

Background: Delayed wound healing in skin injuries has become a significant problem clinics, seriously affecting and even threatening life health. Recently, research interest increased developing dressings containing bioactive compounds capable of improving outcomes for complex needs. Methods: In this study, Puerarin -loaded nanoparticles (Pue-NPs) were prepared using the cell-penetrating peptide-poly (lactic-co-glycolic acid) (CPP-PLGA) as drug carrier by emulsified solvent evaporation method. Then, they added into poly (acrylic to obtain self-assembled nanocomposite hydrogels (SANHs) delivery system co-polymerization The particle size, zeta potential, micromorphology Pue-NPs measured; appearance, mechanical properties, adhesive strength, biological activity SANHs performed. Finally, potential was further evaluated streptozotocin-induced diabetic mice. Results: regularly spherical, with an average size 134.57 ± 1.42 nm 2.14 0.78 mV. colorless transparent honeycomb-like porous structure had excellent swelling ratio (917%), water vapor transmission rate (3077 g·m − 2 ·day 1 ), properties (Young's modulus 18 kPa, elongation at break 307%), strength (15.5 kPa). exhibited sustained release Pue over 48h, cumulative 55.60 6.01%. vitro tests revealed that presented 92.22% antibacterial against Escherichia coli after 4h, 61.91% scavenging 1.1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical. vivo experiments showed accelerated repair reducing inflammatory response site, promoting angiogenesis, facilitating epidermal regeneration collagen deposition. Conclusion: conclusion, we successfully SANHs. Our results show have performance improves mice model, indicating it can be used develop effective strategy treatment wounds. Keywords: nanoparticles, hydrogel, delivery,

Language: Английский

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Puerarin—A Promising Flavonoid: Biosynthesis, Extraction Methods, Analytical Techniques, and Biological Effects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5222 - 5222

Published: May 10, 2024

Flavonoids, a variety of plant secondary metabolites, are known for their diverse biological activities. Isoflavones subgroup flavonoids that have gained attention potential health benefits. Puerarin is one the bioactive isoflavones found in Kudzu root and Pueraria genus, which widely used alternative Chinese medicine, has been to be effective treating chronic conditions like cardiovascular diseases, liver gastric respiratory diabetes, Alzheimer’s disease, cancer. extensively researched both scientific clinical studies over past few years. The purpose this review provide an up-to-date exploration puerarin biosynthesis, most common extraction methods, analytical techniques, effects, new perspective medical pharmaceutical research development.

Language: Английский

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Protective effect of flavonoids on oxidative stress injury in Alzheimer’s disease

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: June 23, 2024

Alzheimer's disease (AD) is the most common neurodegenerative disease, which mainly caused by damage of structure and function central nervous system. At present, there are many adverse reactions in market-available drugs, can't significantly inhibit occurrence AD. Therefore, current focus research to find safe effective therapeutic drugs improve clinical treatment Oxidative stress bridges different mechanism hypotheses AD plays a key role Numerous studies have shown that natural flavonoids good antioxidant effects. They can directly or indirectly resist ­oxidative stress, Aβ aggregation Tau protein hyperphosphorylation activating Nrf2 other oxidation-antioxidation-related signals, regulating synaptic function-related pathways, promoting mitochondrial autophagy, etc., play neuroprotective In this review, we summarised inhibiting oxidative injury recent years. Moreover, because shortcomings poor biofilm permeability low bioavailability flavonoids, advantages progress nano-drug delivery systems such as liposomes solid lipid nanoparticles were highlighted. We hope review provides useful way explore treatments.

Language: Английский

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Puerarin alleviates hyperosmotic stress-induced oxidative stress, inflammation, apoptosis and barrier damage of human corneal epithelial cells by targeting SIRT1/NLRP3 signaling

Toxicology in Vitro, Journal Year: 2023, Volume and Issue: 94, P. 105722 - 105722

Published: Oct. 21, 2023

Language: Английский

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Benefits of Puerarin on Metabolic Syndrome and Its Associated Cardiovascular Diseases in Rats Fed a High-Fat/High-Sucrose Diet

Nutrients, Journal Year: 2024, Volume and Issue: 16(9), P. 1273 - 1273

Published: April 25, 2024

Metabolic syndrome (MetS) is a cluster of risk factors for cardiovascular diseases (CVDs) that has become global public health problem. Puerarin (PUE), the principal active compound Pueraria lobata, effects regulating glucose and lipid metabolism protecting against damage. This study aimed to investigate whether dietary supplementation with PUE could ameliorate MetS its associated Rats were randomly divided into three groups: normal diet group (NC), high-fat/high-sucrose (HFHS), HFHS plus (HFHS-PUE). The results showed PUE-supplemented rats exhibited enhanced tolerance, improved parameters, reduced blood pressure compared those on alone. Additionally, reversed HFHS-induced elevations in atherogenic index (AI) activities serum lactate dehydrogenase (LDH) creatine kinase (CK). Ultrasonic evaluations indicated significantly ameliorated cardiac dysfunction arterial stiffness. Histopathological assessments further confirmed mitigated remodeling, neuronal damage brain. Moreover, lowered systemic inflammatory indices including C-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte (MLR), immune-inflammation (SII). In conclusion, effectively moderated metabolic disorders, attenuated inflammation, minimized induced by an diet. These provide novel insights potential benefits prevention management related CVDs.

Language: Английский

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Puerarin Alleviates Experimental Autoimmune Thyroiditis by Regulating Macrophages

The Journal of Immunology, Journal Year: 2024, Volume and Issue: 212(11), P. 1670 - 1679

Published: April 26, 2024

Abstract Hashimoto’s thyroiditis (HT) is the most common organ-specific autoimmune disease, predominantly affecting women. Although pathogenesis of HT incompletely understood, some studies have found that macrophage polarization plays a role. Puerarin soy isoflavone compound has anti-inflammatory and immunomodulatory effects regulates immune activity. This study aimed to verify therapeutic effect puerarin on explored its regulatory imbalance in HT. Through bioinformatics analysis molecular biology methods, it was macrophages increased significantly patients model mice. Immunological staining showed intervention could reduce tissue inflammatory cell infiltration. Molecular biological examination displayed inhibit local systemic inflammation levels, expression marker thyroglobulin thyroid peroxidase Abs. In vivo experimental results indicated regulated polarity reduced damage, possibly by inhibiting pyroptosis signaling pathway. clearance experiments demonstrated relied exert mechanism action treating The this indicate are important mediators development HT, can regulate status provide protection, which provides new idea for treatment

Language: Английский

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Investigating the Potential Therapeutic Mechanisms of Puerarin in Neurological Diseases

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(12), P. 10747 - 10769

Published: May 23, 2024

Language: Английский

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Targeting m6A mRNA demethylase FTO alleviates manganese-induced cognitive memory deficits in mice

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 476, P. 134969 - 134969

Published: June 18, 2024

Language: Английский

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Gegen Qinlian decoction alleviates depression-like behavior by modulating the gut microenvironment in CUMS rats

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 20, 2024

Gegen Qinlian Decoction (GQD) is a classical traditional Chinese medicine (TCM) formula primarily utilized for treating gut disorders. GQD showed therapeutic effects on several diseases in clinical and animal studies by targeting microbes. Our recent also found that efficiently alleviated anxiety methamphetamine-withdrawn mice via regulating microbiome metabolism. Given various have indicated the link between development of depression, here we endeavor to explore whether can manage depression disorders microbiome.

Language: Английский

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