Repurposed Drugs and Plant-Derived Natural Products as Potential Host-Directed Therapeutic Candidates for Tuberculosis DOI Creative Commons
Rubhana Raqib, Protim Sarker

Biomolecules, Journal Year: 2024, Volume and Issue: 14(12), P. 1497 - 1497

Published: Nov. 24, 2024

Tuberculosis (TB) is one of the leading causes death due to infectious disease. It a treatable disease; however, conventional treatment requires lengthy regimen with severe side effects, resulting in poor compliance among TB patients. Intermittent drug use, non-compliance patients, and prescription errors, other factors, have led emergence multidrug-resistant TB, while mismanagement (MDR-TB) has eventually development extensively drug-resistant tuberculosis (XDR-TB). Thus, there an urgent need for new development, but enormous expenses time required (up 20 years) research therapeutic approaches are required. Host-directed therapies (HDT) could be most attractive strategy, as they target host defense processes instead microbe thereby may prevent alarming rise MDR- XDR-TB. This paper reviews progress HDT using repurposed drugs which been investigated clinical trials (completed or ongoing) plant-derived natural products that preclinical trial stages. Additionally, this review describes existing challenges future directions implementation HDT.

Language: Английский

Antimicrobial Activity of UV-Activated and Cysteamine-Grafted Polymer Foils Against Bacteria and Algae DOI Open Access
Viktorie Neubertová,

Tereza Silovská,

Václav Švorčı́k

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(2), P. 251 - 251

Published: Jan. 20, 2025

Surface modification of various polymer foils was achieved by UV activation and chemical grafting with cysteamine to improve surface properties antimicrobial efficacy. UVC at 254 nm led changes in wettability charge density, which allowed the introduction amino thiol functional groups grafting. X-ray photoelectron spectroscopy (XPS) confirmed increased nitrogen sulfur content on modified surfaces. SEM analysis revealed that resulted distinct roughness texturing, are expected enhance microbial interactions. Antimicrobial tests showed resistance algal growth (inhibition test) bacterial colonization (drop plate method), significant improvement observed for polyethylene terephthalate (PET) polyetheretherketone (PEEK) foils. The important factors influencing efficacy included exposure time concentration, longer higher concentrations leading reduction up 45.7% Escherichia coli 55.6% Staphylococcus epidermidis. These findings highlight potential combining as an effective method developing polymeric materials enhanced function, offering applications industries such healthcare packaging.

Language: Английский

Citations

0
Immunomodulatory effects of cysteamine and its potential use as a host-directed therapy for tuberculosis DOI Creative Commons
Saeid Najafi Fard, Chiara Farroni, Linda Petrone

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 28, 2024

Objective Cysteamine, a drug approved to treat cystinosis, has been proposed as host-directed therapy for M. tuberculosis (Mtb) and SARS-CoV-2. The impact of cysteamine on the immune responses not fully investigated. We aimed in vitro evaluate immunomodulatory effects peripheral blood mononuclear cells (PBMCs) using purified protein derivative (PPD) recall antigen, an unspecific stimulus staphylococcal enterotoxin B (SEB). Methods PBMCs isolated from subjects with infection (TBI), those disease (TB), healthy controls (HC) were stimulated PPD or SEB treated at different concentrations (50 µM–400 µM) 6 hours (h) 24 h. evaluated T helper1 (Th1) cytotoxic1 (Tc1) cell cytokine production by flow cytometry immune-enzymatic assays. In HC, we also apoptosis and/or necrosis cytometry. Results observed effect 400 µM TB TBI subjects. It significantly reduced PPD-specific Th1 h (p=0.0004 p=0.0009, respectively), similar non-significant trend was 200 (p=0.06 p=0.14 h). Moreover, both (p<0.0001 p=0.0187 h, respectively, p<0.0001 both) (p=0.0119 p=0.0028 p=0.0003 respectively) SEB-induced Tc1 responses. Furthermore, found that induced morphological lymphocyte changes percentage dose- time-dependent manner. Cysteamine 8% late 1.6% (p<0.05) contrast, despite significant differences untreated conditions (p<0.05), approximately 1% 0.1% cells. Conclusions High doses reduce percentages PPD- induce necrosis. Differently, lower retains without affecting viability. These findings suggest potential adjunct antimicrobial regimens COVID-19 field, its ability inflammatory status.

Language: Английский

Citations

1
Repurposed Drugs and Plant-Derived Natural Products as Potential Host-Directed Therapeutic Candidates for Tuberculosis DOI Creative Commons
Rubhana Raqib, Protim Sarker

Biomolecules, Journal Year: 2024, Volume and Issue: 14(12), P. 1497 - 1497

Published: Nov. 24, 2024

Tuberculosis (TB) is one of the leading causes death due to infectious disease. It a treatable disease; however, conventional treatment requires lengthy regimen with severe side effects, resulting in poor compliance among TB patients. Intermittent drug use, non-compliance patients, and prescription errors, other factors, have led emergence multidrug-resistant TB, while mismanagement (MDR-TB) has eventually development extensively drug-resistant tuberculosis (XDR-TB). Thus, there an urgent need for new development, but enormous expenses time required (up 20 years) research therapeutic approaches are required. Host-directed therapies (HDT) could be most attractive strategy, as they target host defense processes instead microbe thereby may prevent alarming rise MDR- XDR-TB. This paper reviews progress HDT using repurposed drugs which been investigated clinical trials (completed or ongoing) plant-derived natural products that preclinical trial stages. Additionally, this review describes existing challenges future directions implementation HDT.

Language: Английский

Citations

1