Biotechnology for Biofuels and Bioproducts,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: June 15, 2023
Lignocellulose-derived
aldehyde
inhibitors
seriously
blocked
the
biorefinery
of
biofuels
and
biochemicals.
To
date,
economic
production
lignocellulose-based
products
heavily
relied
on
high
productivities
fermenting
strains.
However,
it
was
expensive
time-consuming
for
achievable
rational
modification
to
strengthen
stress
tolerance
robustness
inhibitors.
Here,
aimed
improve
cellulosic
bioethanol
fermentability
chassis
Zymomonas
mobilis
ZM4
pretreated
using
energy-efficient
eco-friendly
cold
plasma.It
found
that
weaker
in
CSH
(corn
stover
hydrolysates)
than
synthetic
medium
Z.
mobilis,
thus
attributed
inhibition
lignocellulose-derived
CSH.
Convincingly,
further
confirmed
mixed
aldehydes
severely
decreased
accumulation
through
additional
supplementary
assays
medium.
After
assayed
under
different
processing
time
(10-30
s),
discharge
power
(80-160
W),
working
pressure
(120-180
Pa)
atmosphere
plasma
(CAP),
achieved
increased
after
at
optimized
parameters
(20
s,
140
W
165
Pa).
It
showed
brought
about
three
mutation
sites
including
ZMO0694
(E220V),
ZMO0843
(L471L)
(P505H)
via
Genome
resequencing-based
SNPs
(single
nucleotide
polymorphisms).
A
serial
differentially
expressed
genes
(DEGs)
were
identified
as
potential
contributors
RNA-Seq
sequencing,
ZMO0253
ZMO_RS09265
(type
I
secretion
outer
membrane
protein),
ZMO1941
(Type
IV
secretory
pathway
protease
TraF-like
ZMOr003
ZMOr006
(16S
ribosomal
RNA),
ZMO0375
ZMO0374
(levansucrase)
ZMO1705
(thioredoxins).
enriched
cellular
process,
followed
by
metabolic
process
single-organism
biological
process.
For
KEGG
analysis,
mutant
also
referred
starch
sucrose
metabolism,
galactose
metabolism
two-component
system.
Finally,
but
interestingly,
simultaneously
enhanced
capacity
mobilis.Of
several
candidate
genetic
changes,
treated
with
conferred
upon
facilitated
production.
This
work
would
provide
a
strain
biocatalyst
efficient
lignocellulosic
Biotechnology Journal,
Journal Year:
2023,
Volume and Issue:
18(12)
Published: Aug. 3, 2023
Abstract
Background
Environmental
stress
resistance
is
still
a
bottleneck
for
economical
process
l
‐lactic
acid
fermentation.
Chronological
lifespan
(CLS)
extension
has
represented
promising
strategy
improving
of
microbial
cell
factories.
Main
methods
and
major
results
In
this
study,
addition
anti‐aging
drug
cysteine,
kind
extending
CLS
factories,
was
systematically
evaluated
on
viability
production
in
Bacillus
coagulans
CICC
23843.
The
revealed
that
16
m
‐cysteine
supplement
significantly
improved
titer
B.
.
enhanced
total
antioxidant
capacity
(T‐AOC)
key
enzymes
activities
involving
glycolytic
pathway
as
well
differentially
expressed
genes
involved
cysteine
synthesize
precursor
pathways,
fatty
degradation
may
help
to
further
understand
the
relative
mechanism
effect
accumulation.
Finally,
based
supplement,
final
130.5
g
L
−1
with
productivity
4.07
h
conversion
rate
0.94
sugar
achieved
5
bioreactor.
Conclusions
implications
This
study
provided
valuable
option
engineering
lactic
bacteria
enhancement
yield.
Synthetic biology and engineering,
Journal Year:
2023,
Volume and Issue:
1(1), P. 1 - 19
Published: Jan. 1, 2023
Thermoanaerobacter
species,
which
have
broad
substrate
range
and
high
operating
temperature,
can
directly
utilize
lignocellulosic
materials
for
biofuels
production.Compared
with
the
mesophilic
process,
thermophilic
process
shows
greater
prospects
in
consolidated
bioprocessing
(CBP)
due
to
its
relatively
higher
efficiency
of
lignocellulose
degradation
lower
risk
microbial
contamination.Additionally,
conditions
reduce
cooling
costs,
further
facilitate
downstream
product
recovery.This
review
comprehensively
summarizes
advances
species
biorefinery,
including
their
performance
on
substrates
utilization,
genetic
modification
or
other
strategies
enhanced
production.Furthermore,
bottlenecks
sugar
co-fermentation,
metabolic
engineering,
are
also
discussed.
Biotechnology for Biofuels and Bioproducts,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: June 15, 2023
Lignocellulose-derived
aldehyde
inhibitors
seriously
blocked
the
biorefinery
of
biofuels
and
biochemicals.
To
date,
economic
production
lignocellulose-based
products
heavily
relied
on
high
productivities
fermenting
strains.
However,
it
was
expensive
time-consuming
for
achievable
rational
modification
to
strengthen
stress
tolerance
robustness
inhibitors.
Here,
aimed
improve
cellulosic
bioethanol
fermentability
chassis
Zymomonas
mobilis
ZM4
pretreated
using
energy-efficient
eco-friendly
cold
plasma.It
found
that
weaker
in
CSH
(corn
stover
hydrolysates)
than
synthetic
medium
Z.
mobilis,
thus
attributed
inhibition
lignocellulose-derived
CSH.
Convincingly,
further
confirmed
mixed
aldehydes
severely
decreased
accumulation
through
additional
supplementary
assays
medium.
After
assayed
under
different
processing
time
(10-30
s),
discharge
power
(80-160
W),
working
pressure
(120-180
Pa)
atmosphere
plasma
(CAP),
achieved
increased
after
at
optimized
parameters
(20
s,
140
W
165
Pa).
It
showed
brought
about
three
mutation
sites
including
ZMO0694
(E220V),
ZMO0843
(L471L)
(P505H)
via
Genome
resequencing-based
SNPs
(single
nucleotide
polymorphisms).
A
serial
differentially
expressed
genes
(DEGs)
were
identified
as
potential
contributors
RNA-Seq
sequencing,
ZMO0253
ZMO_RS09265
(type
I
secretion
outer
membrane
protein),
ZMO1941
(Type
IV
secretory
pathway
protease
TraF-like
ZMOr003
ZMOr006
(16S
ribosomal
RNA),
ZMO0375
ZMO0374
(levansucrase)
ZMO1705
(thioredoxins).
enriched
cellular
process,
followed
by
metabolic
process
single-organism
biological
process.
For
KEGG
analysis,
mutant
also
referred
starch
sucrose
metabolism,
galactose
metabolism
two-component
system.
Finally,
but
interestingly,
simultaneously
enhanced
capacity
mobilis.Of
several
candidate
genetic
changes,
treated
with
conferred
upon
facilitated
production.
This
work
would
provide
a
strain
biocatalyst
efficient
lignocellulosic
