Cofactor engineering in Thermoanaerobacterium aotearoense SCUT27 for maximizing ethanol yield and revealing an enzyme complex with high ferredoxin-NAD+ reductase activity

Bioresource Technology, Journal Year: 2024, Volume and Issue: 402, P. 130784 - 130784

Published: May 1, 2024

Language: Английский

Metabolic engineering of Thermoanaerobacterium aotearoense strain SCUT27 for biofuels production from sucrose and molasses

Biotechnology for Biofuels and Bioproducts, Journal Year: 2023, Volume and Issue: 16(1)

Published: Oct. 21, 2023

Abstract Background Sucrose-rich sugarcane trash surpasses 28 million tons globally per year. Effective biorefinery systems could convert these biomasses to bioproducts, such as bioethanol from sucrose in Brazil. Thermophilic microbes for biofuels have attracted great attention due their higher fermentation temperature and wide substrate spectrum. However, few thermophiles using or molasses production was reported. Thermoanaerobacterium aotearoense SCUT27 has been considered an efficient ethanol producer, but it cannot directly utilize sucrose. In this study, various metabolic pathways were introduced analyzed Thermoanaerobaterium. Results The sucrose-6-phosphate hydrolase ( scrB ), which a screened strain thermosaccharolyticum G3-1 overexpressed T. endowed with the ability addition, overexpression of sucrose-specific PTS system scrA ) Clostridium acetobutylicum accelerated transport. To strengthen alcohols substrates metabolism, redox-sensing transcriptional repressor rex further knocked out. Moreover, gene arginine argR deleted, ethanologenic mutant P8S10 showed inhibitors-tolerance finally accumulated ~ 34 g/L (a yield 0.39 g/g sugars) pretreated cane 5 L tank by fed-batch fermentation. When introducing butanol synthetic pathway, 3.22 produced P8SB4 0.44 g alcohols/g sugars at 50℃. This study demonstrated potential application low cost molasses. Conclusions Our work provided strategies utilization improved well stress tolerances SCUT27, demonstrating cost-effective sucrose-based feedstocks.

Language: Английский

Adaptive laboratory evolution of Thermoanaerobacterium aotearoense for enhanced ethanol production from raw cassava starch

Renewable Energy, Journal Year: 2024, Volume and Issue: 233, P. 121179 - 121179

Published: Aug. 14, 2024

Language: Английский