Microbiology Research, Journal Year: 2023, Volume and Issue: 14(4), P. 1774 - 1787

Published: Nov. 2, 2023

Geobacter grbiciae can grow via coupling oxidation of ethanol to the reduction various forms soluble Fe(III) and poorly crystalline oxide, suggesting that G. act as an electron-donor microbe for forming co-cultures through direct interspecies electron transfer (DIET). In this report, potential DIET Methanosarcina barkeri 800, sulfurreducens Δhyb, or Methanospirillum hungatei, electron-acceptor microbes, were examined. Co-cultures Δhyb performed with sole substance fumarate in presence granular activated carbon (GAC), magnetite, polyester felt. The conditions co-culturing M. 800 (or hungatei) same those except was absent different cultivation temperatures used. All anaerobically cultivated. Samples regularly withdrawn from monitor methane, fumarate, succinate gas high-performance liquid chromatography. formed functional GAC magnetite. No co-culture H2/formate-utilizing methanogen hungatei observed. Additionally, H2/formate-un-utilizing without magnetite supplement. These findings indicate between 800/G. is rather than H2/formate, confirming acts microbe. Although syntrophically converted methane DIET, conversion propionate butyrate not expand range microbes donors interaction other DIET. However, metabolism mixed microbial communities a product requires further analysis. This study provides framework finding new microbes.

Language: Английский