Bioengineering in Solid-State Fermentation for next sustainable food bioprocessing

Next Sustainability, Journal Year: 2025, Volume and Issue: 6, P. 100105 - 100105

Published: Jan. 1, 2025

Language: Английский

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Enzymes in the bioconversion of food waste into valuable bioproducts: A circular economy perspective

Systems Microbiology and Biomanufacturing, Journal Year: 2024, Volume and Issue: 4(3), P. 850 - 868

Published: June 7, 2024

Language: Английский

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Bioenergy production from chicken feather waste by anaerobic digestion and bioelectrochemical systems

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: April 4, 2024

Abstract Background Poultry feather waste has a potential for bioenergy production because of its high protein content. This research explored the use chicken hydrolysate methane and hydrogen via anaerobic digestion bioelectrochemical systems, respectively. Solid state fermentation was conducted using recombinant strain Bacillus subtilis DB100 (p5.2). Results In digestion, produced maximally 0.67 Nm 3 CH 4 /kg feathers 0.85 mmol H 2 /day.L concomitant to COD removal 86% 93%, The systems used were microbial fuel electrolysis cells. first cell, electricity with maximum cell 375 mV current 0.52 mA. enhanced rate 7.5 mmol/day.L, density 11.5 A/m power 9.26 W/m . Conclusions data indicated that sustainable utilization keratin produce biohydrogen bioelectrical chemical is feasible. Keratin can biofuels through an integrated aerobic-anaerobic system. Graphical

Language: Английский

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Biogas production from chicken feathers via pretreatment with keratinolytic protease secreting bacteria

Biomass Conversion and Biorefinery, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

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Protein engineering, expression optimization, and application of alkaline protease from Alkalihalobacillus clausii FYX

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141891 - 141891

Published: March 1, 2025

Language: Английский

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Redox and solvent-stable alkaline serine protease from Bacillus patagoniensis DB-5: heterologous expression, properties, and biotechnological applications

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

The aprBP gene from Bacillus patagoniensis DB-5, encoding a 378-amino-acid alkaline protease, was cloned and expressed in Escherichia coli . amino acid sequence of APrBP showed 62.8–84.4% identity with the S8 peptidase subtilisin family proteases reported literature. Recombinant purified using Ni-NTA affinity chromatography 45.61% recovery homogeneous band detected at approximately 38 kDa on SDS-PAGE gel. optimum temperature 60°C. presence 2 mM Ca 2+ significantly enhanced optimal thermostability. enzyme demonstrated activity pH 12 maintained high stability 8.0–11.0. Protease stimulated by Mn , Mg Ni TritonX-100, Tween-20 Tween-80, while completely inactivated PMSF, EDTA Cu exhibited good tolerance to oxidizing reducing agents. Notably, protease remarkable 50% (v/v) concentrations several organic solvents, such as methanol, acetone, glycerol, dimethyl sulfoxide, n-hexane, ethyl acetate. efficiently hydrolyzed natural proteins, demonstrating highest catalytic efficiency for casein, excellent hydrolysis bovine serum albumin, hemoglobin, keratin, favorable ability whey proteins. Moreover, molecular docking results revealed stable interactions between proteins keratin. This study indicated that has some useful properties explored its potential bio-additive detergent well utilizing feather waste protein.

Language: Английский

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From laboratory-level construction to pilot-level validation: Utilization of compound mutagenic bacterial agent for efficient treatment of kitchen waste

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 116441 - 116441

Published: April 1, 2025

Language: Английский

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Fermentation of feathers with Bacillus sp. TC5 to simultaneous obtain keratinase and antioxidant-rich peptide products

Biomass Conversion and Biorefinery, Journal Year: 2024, Volume and Issue: 15(5), P. 7435 - 7451

Published: May 3, 2024

Language: Английский

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Prospects and Application of Solid-State Fermentation in Animal Feed Production – A Review

Annals of Animal Science, Journal Year: 2024, Volume and Issue: 24(4), P. 1123 - 1137

Published: Feb. 27, 2024

Abstract Animal feed production has recently received significant attention in the food and livestock sectors due to its high nutritional value substantial environmental potential. Extensive studies have been conducted explore effects of solid fermented feeds on different growth stages animals but also health status animals, quality animal products, composition intestinal micro-flora replace non-fermented production. The purpose this review is provide up-to-date existing trends, recent developments, prospects solid-state fermentation (SSF) practices for feeds. Studies enhancing factors by increasing crude protein content, enzymes, antioxidant activity using physical processing methods agro-industrial waste such as rapeseed meal, cottonseed, wheat bran, soybean legumes mainly SSF are reviewed discussed thoroughly.

Language: Английский

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Cloning Systems in Bacillus: Bioengineering of Metabolic Pathways for Valuable Recombinant Products

Fermentation, Journal Year: 2024, Volume and Issue: 10(1), P. 50 - 50

Published: Jan. 9, 2024

Representatives of the genus Bacillus have been established as one most important industrial microorganisms in last few decades. Genetically modified B. subtilis and, to a lesser extent, licheniformis, amyloliquefaciens, and megaterium used for heterologous expression numerous proteins (enzymes, vaccine components, growth factors), platform chemicals, other organic compounds importance. Vectors designed work spp. dramatically increased number complexity. Today, they provide opportunities genetic manipulation on every level, from point mutations systems biology, that were impossible even ten years ago. The present review aims describe concisely latest developments shuttle, integrative, CRISPR-Cas9 vectors well their application large-scale bioengineering with prospect producing valuable an scale. Genetic manipulations promoters vectors, together impact secretory metabolic pathways, are discussed detail.

Language: Английский

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