Generation of a Vibrio-based Platform for Efficient Conversion of Raffinose through Adaptive Laboratory Evolution on a Solid Medium DOI
Sunghwa Woo, Yong Hee Han, Hye Kyung Lee

et al.

Metabolic Engineering, Journal Year: 2024, Volume and Issue: 86, P. 300 - 307

Published: Nov. 1, 2024

Language: Английский

Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio sp. dhg DOI Creative Commons

Yang Jun Shon,

Dongyeop Baek, Shouguang Jin

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Background Vibrio sp. dhg is a fast-growing, alginate-utilizing, marine bacterium being developed as platform host for macroalgae biorefinery. To maximize its potential in the production of various value-added products, there need to expand genetic engineering tools versatile editing.Results The CRISPR-based cytosine base editing (CBE) system was established dhg, enabling C:G-to-T:A point mutations multiple genomic loci. This CBE displayed high efficiencies single and targets, reaching up 100%. efficiently introduced premature stop codons, inactivating seven genes encoding putative restriction enzymes restriction-modification two rounds. A resulting engineered strain significantly enhanced transformation efficiency by 55.5-fold.Conclusions Developing highly efficient improving enable manipulation diverse brown bioconversion.

Language: Английский

Citations

0
Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg DOI Creative Commons

Yang Jun Shon,

Dongyeop Baek, Shouguang Jin

et al.

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: April 9, 2025

Abstract Background Vibrio sp. dhg is a fast-growing, alginate-utilizing, marine bacterium being developed as platform host for macroalgae biorefinery. To maximize its potential in the production of various value-added products, there need to expand genetic engineering tools versatile editing. Results The CRISPR-based cytosine base editing (CBE) system was established dhg, enabling C: G-to-T: A point mutations multiple genomic loci. This CBE displayed high efficiencies single and targets, reaching up 100%. efficiently introduced premature stop codons, inactivating seven genes encoding putative restriction enzymes restriction-modification two rounds. resulting engineered strain significantly enhanced transformation efficiency by 55.5-fold. Conclusions Developing highly efficient improving enable manipulation diverse brown bioconversion.

Language: Английский

Citations

0
Recent Advances in Alginate Lyase Engineering for Efficient Conversion of Alginate to Value‐Added Products DOI Creative Commons

Hye‐Min Shin,

Jo Hyun Moon, Sunghwa Woo

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(5)

Published: April 28, 2025

ABSTRACT Alginate lyases depolymerize alginate and generate oligosaccharides (AOS) eventually 4‐deoxy‐L‐erythro‐5‐hexoseulose uronate (DEH), a monosaccharide. Recently, have garnered significant attention due to the increasing demand for AOS, which exhibit bioactivities beneficial human health, livestock productivity, agricultural efficiency. Additionally, these enzymes play crucial role in producing DEH, essential catabolism bacteria. This review explains industrial value of AOS contribute broadly industries ranging from food industry biorefinery processes. also highlights recent advances lyase applications engineering, including domain truncation, chimeric enzyme design, rational mutagenesis, directed evolution. These approaches enhanced performance efficient DEH production. We discuss current challenges future directions toward industrial‐scale bioconversion alginate‐rich biomass.

Language: Английский

Citations

0
Direct Itaconate Production from Brown Macroalgae Using Engineered Vibrio sp. dhg DOI
Jo Hyun Moon, Sunghwa Woo, Hyojeong Shin

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(30), P. 16860 - 16866

Published: July 20, 2024

Itaconate is a promising platform chemical with broad applicability, including the synthesis of poly(methyl methacrylate). Most studies on microbial itaconate production entail use crop-based feedstock, which imposes constraints due to its limited supply. Brown macroalgae have recently gained attention as next-generation biomass owing their high productivity and carbohydrate content amenability mass production. Therefore, for warrants exploration. In this study, direct from brown was demonstrated using engineered

Language: Английский

Citations

3
Engineering of fast-growing Vibrio natriegens for biosynthesis of poly(3-hydroxybutyrate-co-lactate) DOI Creative Commons

Xinye Sun,

Yanzhe Shang,

Binghao Zhang

et al.

Bioresources and Bioprocessing, Journal Year: 2024, Volume and Issue: 11(1)

Published: Sept. 9, 2024

Abstract Poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] is a highly promising valuable biodegradable material with good biocompatibility and degradability. Vibrio natriegens , owing to its fast-growth, wide substrate spectrum characteristics, was selected produce P(3HB-co-LA). Herein, the crucial role of acetyltransferase PN96-18060 for PHB synthesis in V. identified. Heterologous pathway P(3HB-co-LA) introduced into successfully, addition, overexpression dldh gene led 1.84 fold enhancement lactate content Finally, production characterized under different carbon sources. The fraction increased 28.3 mol% by modification, about times that control. This first successful case producing . Collectively, this study showed an attractive host organism has great potential other co-polymers.

Language: Английский

Citations

3
Direct Degradation of Fresh and Dried Macroalgae by Agarivorans albus B2Z047 DOI Creative Commons
Ya Gong,

Dan-Dan Shang,

Chenglin Sun

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(5), P. 203 - 203

Published: April 28, 2024

Marine macroalgae are increasingly recognized for their significant biological and economic potential. The key to unlocking this potential lies in the efficient degradation of all carbohydrates from biomass. However, a variety polysaccharides (alginate, cellulose, fucoidan, laminarin), difficult degrade simultaneously short time. In study, brown alga Saccharina japonica was found be rapidly thoroughly degraded by marine bacterium Agarivorans albus B2Z047. This strain harbors broad spectrum carbohydrate-active enzymes capable degrading various polysaccharides, making it uniquely equipped efficiently break down both fresh dried kelp, achieving hydrolysis rate up 52%. A transcriptomic analysis elucidated presence pivotal enzyme genes implicated pathways alginate, laminarin. discovery highlights bacterium's capability comprehensive conversion kelp biomass, indicating its biotechnological applications resource utilization.

Language: Английский

Citations

2
Advancing Bacillus licheniformis as a Superior Expression Platform through Promoter Engineering DOI Creative Commons

Fengxu Xiao,

Yupeng Zhang, Lihuan Zhang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1693 - 1693

Published: Aug. 16, 2024

is recognised as an exceptional expression platform in biomanufacturing due to its ability produce high-value products. Consequently, metabolic engineering of

Language: Английский

Citations

1
Systematic Review on Marine Carbon Source-Mannitol:Applications in Synthetic Biology DOI

Fengxu Xiao,

Yupeng Zhang, Lihuan Zhang

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127881 - 127881

Published: Aug. 31, 2024

Language: Английский

Citations

1
Engineering a non-oxidative glycolysis pathway in escherichia coli for high-level citramalate production DOI Creative Commons
Tingting Wang, Lijuan Ding, Huiying Luo

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Aug. 22, 2024

Methyl methacrylate (MMA) is a key precursor of polymethyl methacrylate, extensively used as transparent thermoplastic in various industries. Conventional MMA production poses health and environmental risks; hence, citramalate serves an alternative bacterial compound for production. The highest titer was previously achieved by Escherichia coli BW25113. However, studies on further improving through metabolic engineering are limited, phage contamination persistent problem E. fermentation. This study aimed to construct phage-resistant BW25113 strain capable producing high titers from glucose. First, promoters heterologous cimA genes were screened, effective biosynthetic pathway established overexpressing MjcimA3.7, mutated gene Methanococcus jannaschii, regulated the BBa_J23100 promoter coli. Subsequently, engineered integrating Ssp defense system into genome mutating components infection cycle. Then, include non-oxidative glycolysis while removing acetate synthesis enhance supply acetyl-CoA. Furthermore, glucose utilization improved, thereby increasing Ultimately, 110.2 g/L obtained after 80 h fed-batch yield productivity 0.4 g/g 1.4 g/(L·h), respectively. reported without addition expensive yeast extract additional induction fed-bath fermentation, emphasizing its potential practical applications derivatives.

Language: Английский

Citations

0
Generation of a Vibrio-based Platform for Efficient Conversion of Raffinose through Adaptive Laboratory Evolution on a Solid Medium DOI
Sunghwa Woo, Yong Hee Han, Hye Kyung Lee

et al.

Metabolic Engineering, Journal Year: 2024, Volume and Issue: 86, P. 300 - 307

Published: Nov. 1, 2024

Language: Английский

Citations

0