Recent advances in point-of-care testing of COVID-19

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(24), P. 8500 - 8530

Published: Jan. 1, 2023

This review explores various point-of-care optical diagnostic systems combined with microdevices developed during the recent COVID-19 pandemic for clinical diagnostics.

Language: Английский

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Impact of Point-of-Care Testing on Diagnosis, Treatment, and Surveillance of Vaccine-Preventable Viral Infections

Diagnostics, Journal Year: 2025, Volume and Issue: 15(2), P. 123 - 123

Published: Jan. 7, 2025

With the advent of a variety vaccines against viral infections, there are multiple viruses that can be prevented via vaccination. However, breakthrough infections or uncovered strains still cause vaccine-preventable (VPVIs). Therefore, timely diagnosis, treatment, and surveillance these is critical to patient care public health. Point-of-care (POC) diagnostics tools have brought significant improvements in detection management VPVIs. These cutting-edge technologies enable prompt accurate results, enhancing by facilitating treatment decisions. This review delves into advancements POC testing, including antigen/antibody molecular assays, while focusing on their impact VPVIs such as mpox, hepatitis, influenza, flaviviruses (dengue, Zika, yellow fever virus), COVID-19. The role tests monitoring infection crucial for tracking disease progression managing outbreaks. Furthermore, application has shown vital health strategies. In this review, we also highlight emerging CRISPR-based smartphone-integrated devices, which proven particularly beneficial resource-limited settings. We underscore importance continued research optimize diagnostic wider global use dengue, COVID-19, addressing current challenges related sensitivity, specificity, availability, efficiency, more.

Language: Английский

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Catalytic hairpin self-assembly regulated chameleon silver nanoclusters for the ratiometric detection of CircRNA

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 209, P. 114258 - 114258

Published: April 12, 2022

Language: Английский

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Multiplex bacteria detection using one-pot CRISPR/Cas13a-based droplet microfluidics

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 243, P. 115771 - 115771

Published: Oct. 18, 2023

Language: Английский

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Review of paper-based microfluidic analytical devices for in-field testing of pathogens

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1278, P. 341614 - 341614

Published: July 11, 2023

Pathogens cause various infectious diseases and high morbidity mortality which is a global public health threat. The highly sensitive specific detection of significant importance for the effective treatment intervention to minimise impact. However, conventional methods including culture molecular method gravely depend on expensive equipment well-trained skilled personnel, limiting in laboratory. It remains challenging adapt resource-limiting areas, e.g., low middle-income countries (LMICs). To this end, low-cost, rapid, tools with capability field testing portable device identification quantification pathogens, has attracted increasing attentions. Recently, paper-based microfluidic analytical devices (μPADs) have shown promising tool rapid on-site diagnosis, providing cost-effective approach pathogens detection. fast turn-round data collection may also contribute better understanding risks insights mitigation method. In paper, critical developments μPADs in-field both clinical diagnostics environmental surveillance are reviewed. future development, challenges onsite discussed, using cross-disciplinary development with, emerging techniques such as deep learning Internet Things (IoT).

Language: Английский

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Ultrafast Plasmonic Nucleic Acid Amplification and Real-Time Quantification for Decentralized Molecular Diagnostics

ACS Nano, Journal Year: 2023, Volume and Issue: 17(7), P. 6507 - 6518

Published: March 27, 2023

Point-of-care real-time reverse-transcription polymerase chain reaction (RT-PCR) facilitates the widespread use of rapid, accurate, and cost-effective near-patient testing that is available to public. Here, we report ultrafast plasmonic nucleic acid amplification quantification for decentralized molecular diagnostics. The RT-PCR system features an thermocycler (PTC), a disposable plastic-on-metal (PoM) cartridge, ultrathin microlens array fluorescence (MAF) microscope. PTC provides photothermal cycling under white-light-emitting diode illumination precise temperature monitoring with integrated resistance detector. PoM thin film cartridge allows rapid heat transfer as well complete light blocking from excitation source, resulting in highly efficient PCR quantification. Besides, MAF microscope exhibits close-up high-contrast microscopic imaging. All systems were fully packaged palm size point-of-care testing. demonstrates diagnosis coronavirus disease-19 RNA virus within 10 min yields 95.6% efficiency, 96.6% classification accuracy preoperational test, 91% total percent agreement clinical diagnostic test. compact can decentralize primary care developing countries.

Language: Английский

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Metasurfaces for Quantitative Biosciences of Molecules, Cells, and Tissues: Sensing and Diagnostics

ACS Photonics, Journal Year: 2024, Volume and Issue: 11(3), P. 904 - 916

Published: Feb. 23, 2024

Metasurfaces have revolutionized optical biosensing and diagnostic assays due to their sensitivity, compactness, label-free operation. However, metasurface applications in analyzing complex biological systems quantum bioscience phenomena remain scarce. In this Perspective, we discuss current developments biosensors propose promising future for probing or adopting effects improving spatial omics analysis live cells tissues. We the capabilities of platforms monitoring relevant biomarkers viral diseases, neurodegenerative cancers. Metasurface-empowered examination cell morphology secretome, virus detection, tissue imaging can improve accuracy early diagnosis. Furthermore, review device-integration approaches point-of-care testing settings that could constitute pathways toward technology commercialization. Altogether, provide a perspective exploring precision health medicine.

Language: Английский

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A review of the recent achievements and future trends on 3D printed microfluidic devices for bioanalytical applications

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1299, P. 342429 - 342429

Published: Feb. 28, 2024

Language: Английский

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A universal nucleic acid detection platform combing CRISPR/Cas12a and strand displacement amplification with multiple signal readout

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125922 - 125922

Published: March 18, 2024

Language: Английский

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Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies

Diagnostics, Journal Year: 2024, Volume and Issue: 14(5), P. 519 - 519

Published: Feb. 29, 2024

The urgent need for accurate COVID-19 diagnostics has led to the development of various SARS-CoV-2 detection technologies. Real-time reverse transcriptase polymerase chain reaction (RT-qPCR) remains a reliable viral gene technique, while other molecular methods, including nucleic acid amplification techniques (NAATs) and isothermal techniques, provide diverse effective approaches. Serological assays, detecting antibodies in response infection, are crucial disease surveillance. Saliva-based immunoassays show promise surveillance purposes. efficiency antibody varies, with IgM indicating recent exposure IgG offering prolonged detectability. Various rapid tests, lateral-flow immunoassays, present opportunities quick diagnosis, but their clinical significance requires validation through further studies. Challenges include variations specificity sensitivity among testing platforms evolving assay sensitivities over time. antigens, particularly N S proteins, play role diagnostic methods. Innovative approaches, such as nanozyme-based assays specific nucleotide aptamers, offer enhanced flexibility. In conclusion, ongoing advancements methods contribute global effort combating pandemic.

Language: Английский

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