Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(15), P. 3328 - 3352
Published: Jan. 1, 2023
Advanced printable biosensors: paper as example.
Language: Английский
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 259, P. 116374 - 116374
Published: May 10, 2024
Laboratory-based nucleic acid amplification tests (NAATs) are highly sensitive and specific, but they require the transportation of samples to centralized testing facilities have long turnaround times. During Coronavirus Disease 2019 (COVID-19) pandemic, substantial advancement has been achieved with development paper-based point-of-care (POC) NAATs, offering features such as low cost, being easy use, providing rapid sample-to-answer Although most POC NAATs innovations towards clinical settings, we developed a portable, loop-mediated isothermal (LAMP) platform for on-farm applications, capable detecting Bacteroidales fecal contamination biomarker. Our integrated includes drop generator, heating imaging unit, biosensors, results (limit detection 3 copies per cm
Language: Английский
Citations
9
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Rapid and accurate molecular diagnostics are crucial for preventing the global spread of emerging infectious diseases. However, current gold standard nucleic acid detection, reverse transcription polymerase chain reaction (RT-PCR), relies heavily on traditional magnetic beads or silica membranes extraction, resulting in several limitations, including time-consuming processes, need trained personnel, complex equipment. Therefore, there is an urgent fully integrated detection technologies that simple to operate, rapid, highly sensitive meet unmet clinical needs. In this study, we developed a novel, microfluidic cartridge featuring unique needle-plug/piston microvalve, which enables stable long-term reagent storage flexible liquid handling on-site analysis. Coupled with
Language: Английский
Citations
1
Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 343687 - 343687
Published: Jan. 1, 2025
Language: Английский
Citations
1
Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 343752 - 343752
Published: Jan. 1, 2025
Language: Английский
Citations
1
Micromachines, Journal Year: 2022, Volume and Issue: 13(8), P. 1238 - 1238
Published: Aug. 1, 2022
A microfluidic chip is a tiny reactor that can confine and flow specific amount of fluid into channels tens to thousands microns as needed precisely control flow, pressure, temperature, etc. Point-of-care testing (POCT) requires small equipment, has short cycles, controls the process, allowing single or multiple laboratory facilities simultaneously analyze biological samples diagnose infectious diseases. In general, rapid detection stage assessment viral epidemics are essential overcome pandemic situations promptly. Therefore, combining devices with POCT improves efficiency convenience for disease SARS-CoV-2. At same time, chips increases user accessibility, accuracy sensitivity, shortens etc., which beneficial in detecting This review shares recent advances POCT-based COVID-19 how it better suited help response ongoing pandemic.
Language: Английский
Citations
32
Biosensors, Journal Year: 2022, Volume and Issue: 12(12), P. 1094 - 1094
Published: Nov. 29, 2022
Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances. The unique properties paper-based biosensors, including low cost, portability, disposability, and ease use, make them an excellent tool point-of-care testing. Among all analyte methods, nucleic acid-based pathogen offers versatility due to acid synthesis. In a testing context, combination platform allows accurate detection. This review overview contemporary detecting acids from pathogens. methods limitations implementing integrated portable discussed. concludes with potential directions future research in development biosensors.
Language: Английский
Citations
32
Biosensors, Journal Year: 2023, Volume and Issue: 13(2), P. 208 - 208
Published: Jan. 30, 2023
The accurate and rapid diagnosis of viral diseases has garnered increasing attention in the field biosensors. development highly sensitive, selective, accessible biosensors is crucial for early disease detection preventing mortality. However, developing optimized several limitations, including mutations. For decades, nanotechnology been applied numerous biological fields such as biosensors, bioelectronics, regenerative medicine. Nanotechnology offers a promising strategy to address current limitations conventional nucleic acid-based implementation nanotechnologies, functional nanomaterials, nanoplatform-fabrication techniques, surface nanoengineering, not only improved performance but also expanded range sensing targets. Therefore, deep understanding combination nanotechnologies required prepare sanitary emergencies recent COVID-19 pandemic. In this review, we provide interdisciplinary information on nanotechnology-assisted First, representative are discussed, after which review summarizes various acid expect that will valuable basis novel
Language: Английский
Citations
22
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(5), P. 3098 - 3107
Published: Jan. 24, 2023
Nowadays, infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have constituted a new challenge for anti-infective treatment. Precise identification and rapid clinical diagnostics of MRSA from other methicillin-sensitive strains entail assays with robust diagnostic efficiency simple operation steps. Sensitive detection MecA gene is promising to indicate infection, but it challenged the lack isothermal strategies. A visual assay based on rolling circular amplification G-quadruplex/hemin (G4/hemin) DNAzyme proximity assembly was proposed immediate, efficient, cost-effective in steps single tube. The presence specifically drove formation templates, which further triggered amplification. amplified product offered abundant binding sites DNA-grafted hemin probes form novel proximity-assembled G4/hemin structure colorimetric changing diagnosis. This tandem-repeated possessed higher catalytic activity lower background signal than traditional DNAzyme, ensuring sensitive discrimination (limit detection: 9.6 pM). Assay stability antimatrix interference capability enable application, shows compared ability classic methods (100% sensitivity 100% specificity) possesses more simplified procedures shorter turnaround time (<6 h). strategy nonsite-specific hypersensitive manner holds foreseeable prospects research applications.
Language: Английский
Citations
19
Journal of Pharmaceutical Analysis, Journal Year: 2023, Volume and Issue: 13(11), P. 1252 - 1268
Published: Aug. 31, 2023
Waterborne viruses that can be harmful to human health pose significant challenges globally, affecting care systems and the economy. Identifying these waterborne pathogens is essential for preventing diseases protecting public health. However, handling complex samples such as wastewater challenging due their dynamic composition ultralow concentration of target analytes. This review presents a comprehensive overview latest breakthroughs in virus biosensors. It begins by highlighting several promising strategies enhance sensing performance optical electrochemical biosensors samples. These include optimizing bioreceptor selection, transduction elements, signal amplification, integrated systems. Furthermore, insights gained from biosensing are applied improve wastewater, with particular focus on sampling sample pretreatment dispersion characteristics wastewater. suggests implementing system integrates entire detection process high-accuracy analysis could monitoring. findings provide valuable improving effectiveness detection, which have implications environmental management.
Language: Английский
Citations
18
ACS Sensors, Journal Year: 2024, Volume and Issue: 9(2), P. 646 - 653
Published: Jan. 5, 2024
Nucleic acid analysis with an easy-to-use workflow, high specificity and sensitivity, independence of sophisticated instruments, accessibility outside the laboratory is highly desirable for detection monitoring infectious diseases. Integration laboratory-quality sample preparation on a hand-held system critical performance. A SlipChip device inspired by combination lock can perform magnetic bead-based nucleic extraction several clockwise counterclockwise rotations. palm-sized base station was developed to assist provide thermal control isothermal amplification without plug-in power. The loop-mediated reaction be performed colorimetric method directly analyzed naked eye or mobile phone app. This achieves good bead recovery during workflow has minimal residue carryover from lysis elution buffers. Its performance comparable that standard protocol real-time qPCR methods. entire completed in less than 35 min achieve 500 copies/mL sensitivity. Thirty clinical nasal swab samples were collected tested sensitivity 95% 100% SARS-CoV-2. combination-lock provides promising fast, test produces results point-of-care settings, especially home use applications.
Language: Английский
Citations
8