A gamepad-like nucleic acid testing device for rapid detection of SARS-CoV-2 via visible nested recombinase polymerase amplification DOI Creative Commons
Ce Liu,

Baobao Lin,

Guohua Yuan

et al.

Communications Engineering, Journal Year: 2024, Volume and Issue: 3(1)

Published: June 21, 2024

Abstract Nucleic acid tests are essential for the accurate diagnosis and control of infectious diseases. However, current assays not easily scalable a large population, due to requirement laboratory settings or special equipment. Here, we developed an integrated box instant nucleic screening which fully integrates release, amplification, results visualization self-service standalone test. Importantly, operation runs on novel gamepad-like interface, allows deployment in home by users without any prior professional training. The performance is empowered RNA extraction-free sample inactivation process nested recombinase polymerase amplification chemistry exhibits sensitivity comparable reverse transcription-quantitative chain reaction with high specificity severe acute respiratory syndrome coronavirus 2 time 30 minutes directly from fresh swab results. These innovations make platform convenient, accurate, deployable point-of-care testing scheme.

Language: Английский

Advancements in food quality monitoring: integrating biosensors for precision detection DOI Creative Commons
Soumitra Nath

Sustainable Food Technology, Journal Year: 2024, Volume and Issue: 2(4), P. 976 - 992

Published: Jan. 1, 2024

The integration of advanced biosensors enhances the detection contaminants in food. This approach addresses challenges related to sensitivity, specificity, and environmental factors, ensuring food safety quality.

Language: Английский

Citations

21
CRISPR-Microfluidics Nexus: Advancing Biomedical Applications for Understanding and Detection DOI Creative Commons

ZahraSadat Razavi,

M. Soltani, Hamidreza Pazoki‐Toroudi

et al.

Sensors and Actuators A Physical, Journal Year: 2024, Volume and Issue: 376, P. 115625 - 115625

Published: June 24, 2024

The integration of CRISPR technology with microfluidic-based biosensors has greatly expanded its applications in medicine and molecular biology. This combination offers enhanced sensitivity selectivity medical diagnostics. CRISPR-powered microfluidics enables the accurate quantification DNA RNA associated diseases such as cancer, viral infections, bacterial diseases. precise targeting capabilities allow for detection specific genetic sequences, aiding early diagnosis, disease monitoring, treatment assessment. improves specificity by leveraging programmable nature coupling it signal readouts. However, challenges assay optimization, standardization, device fabrication need to be addressed. Additionally, complexity presents potential limitations terms off-target effects unintended modifications. Overall, holds tremendous diagnostics, but further research development are required fully exploit benefits clinical settings.

Language: Английский

Citations

21
Integration of microfluidic channel on electrochemical-based nanobiosensors for monoplex and multiplex analyses: An overview DOI

Hussaini Adam,

Subash C.B. Gopinath, M. K. Md Arshad

et al.

Journal of the Taiwan Institute of Chemical Engineers, Journal Year: 2023, Volume and Issue: 146, P. 104814 - 104814

Published: April 6, 2023

Language: Английский

Citations

41
An update on pathogenesis and clinical scenario for Parkinson’s disease: diagnosis and treatment DOI Open Access

Hussaini Adam,

Subash C.B. Gopinath, M. K. Md Arshad

et al.

3 Biotech, Journal Year: 2023, Volume and Issue: 13(5)

Published: April 27, 2023

Language: Английский

Citations

28
A paper-based loop-mediated isothermal amplification assay for highly pathogenic avian influenza DOI Creative Commons
Mohamed Gomaa Kamel, Josiah Levi Davidson, J M Schober

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 9, 2025

Avian influenza outbreaks have had significant economic and public health consequences worldwide. Therefore, prompt, reliable, cost-effective diagnostic devices are crucial for scrutinizing confining highly pathogenic avian viruses (HPAIVs). Our study introduced evaluated a novel paper-based loop-mediated isothermal amplification (LAMP) test diagnosing the H5 subtype of virus (AIV). We meticulously designed screened LAMP primers targeting H5-haemagglutinin (H5-HA) gene AIV fine-tuned detection assay best performance. The demonstrated limit 500 copies per reaction (25 copies/µl). Additionally, exhibited no cross-reactivity with common bovine pathogens, confirming its specificity. Spiking experiments revealed that could accurately detect 1000 synthetic HPAIV RNA (per reaction) when spiked into oropharyngeal swab samples, achieving 100% analytical sensitivity, specificity, accuracy. This inexpensive, user-friendly point-of-need tool holds great promise, especially in resource-limited settings. It only requires water bath incubation enables visual results without special equipment. Overall, provides promising method rapidly reliably detecting AIV, contributing to improved surveillance early intervention strategies.

Language: Английский

Citations

2
Progress in Fluorescence Biosensing and Food Safety towards Point-of-Detection (PoD) System DOI Creative Commons
Saloni Kakkar, Payal Gupta, Navin Kumar

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(2), P. 249 - 249

Published: Feb. 9, 2023

The detection of pathogens in food substances is crucial concern for public health and the safety natural environment. Nanomaterials, with their high sensitivity selectivity have an edge over conventional organic dyes fluorescent-based methods. Advances microfluidic technology biosensors taken place to meet user criteria sensitive, inexpensive, user-friendly, quick detection. In this review, we summarized use fluorescence-based nanomaterials latest research approaches towards integrated biosensors, including microsystems containing detection, various model systems nano materials, DNA probes, antibodies. Paper-based lateral-flow test strips microchips as well most-used trapping components are also reviewed, possibility performance portable devices evaluated. We present a current market-available system which was developed screening highlight future direction development on-site stratification common foodborne pathogens.

Language: Английский

Citations

24
A portable, easy-to-use paper-based biosensor for rapid in-field detection of fecal contamination on fresh produce farms DOI Creative Commons
Jiangshan Wang, Simerdeep Kaur, Ashley Kayabasi

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 259, P. 116374 - 116374

Published: May 10, 2024

Laboratory-based nucleic acid amplification tests (NAATs) are highly sensitive and specific, but they require the transportation of samples to centralized testing facilities have long turnaround times. During Coronavirus Disease 2019 (COVID-19) pandemic, substantial advancement has been achieved with development paper-based point-of-care (POC) NAATs, offering features such as low cost, being easy use, providing rapid sample-to-answer Although most POC NAATs innovations towards clinical settings, we developed a portable, loop-mediated isothermal (LAMP) platform for on-farm applications, capable detecting Bacteroidales fecal contamination biomarker. Our integrated includes drop generator, heating imaging unit, biosensors, results (limit detection 3 copies per cm

Language: Английский

Citations

9
Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics DOI Creative Commons
Maria‐Nefeli Tsaloglou, Dionysios C. Christodouleas,

Jonathan Milette

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A post-pandemic perspective on paper-based microfluidics by George Whitesides and collaborators. Sketch generated artificial intelligence using an original photograph courtesy of Volker Steger (Science Photo Library).

Language: Английский

Citations

1
Advancing Microfluidic Immunity Testing Systems: New Trends for Microbial Pathogen Detection DOI Creative Commons
Yiran Wang, Jingwei Chen, Yule Zhang

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(14), P. 3322 - 3322

Published: July 15, 2024

Pathogenic microorganisms play a crucial role in the global disease burden due to their ability cause various diseases and spread through multiple transmission routes. Immunity tests identify antigens related these pathogens, thereby confirming past infections monitoring host's immune response. Traditional pathogen detection methods, including enzyme-linked immunosorbent assays (ELISAs) chemiluminescent immunoassays (CLIAs), are often labor-intensive, slow, reliant on sophisticated equipment skilled personnel, which can be limiting resource-poor settings. In contrast, development of microfluidic technologies presents promising alternative, offering automation, miniaturization, cost efficiency. These advanced methods poised replace traditional by streamlining processes enabling rapid, high-throughput immunity testing for pathogens. This review highlights latest advancements systems designed rapid testing, incorporating immunosensors, single molecule arrays (Simoas), lateral flow assay (LFA), smartphone integration. It focuses key pathogenic such as SARS-CoV-2, influenza, ZIKA virus (ZIKV). Additionally, discusses challenges, commercialization prospects, future directions advance infectious detection.

Language: Английский

Citations

5
Interactions between Humans and Dogs during the COVID-19 Pandemic: Recent Updates and Future Perspectives DOI Creative Commons
Mohamed Kamel, Amr El-Sayed, Rachel A. Munds

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(3), P. 524 - 524

Published: Feb. 2, 2023

COVID-19 is one of the deadliest epidemics. This pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but role dogs in spreading disease human society poorly understood. review sheds light on limited susceptibility to infections which likely attributed relatively low levels angiotensin-converting enzyme (ACE2) tract and phylogenetic distance ACE2 from receptor. The affect binding affinity between spike proteins resulting it being uncommon for spread disease. To demonstrate COVID-19, we reviewed epidemiological studies prevalence SARS-CoV-2 dogs. Additionally, discussed use detection as a rapid reliable method effectively discriminating infected non-infected individuals using different types samples (secretions, saliva, sweat). We considered available information human–dog interfaces involving possibility transmission vice versa, behavior changes, importance preventive measures because risk domestic remains concern.

Language: Английский

Citations

11