Communications Engineering,
Journal Year:
2024,
Volume and Issue:
3(1)
Published: June 21, 2024
Abstract
Nucleic
acid
tests
are
essential
for
the
accurate
diagnosis
and
control
of
infectious
diseases.
However,
current
assays
not
easily
scalable
a
large
population,
due
to
requirement
laboratory
settings
or
special
equipment.
Here,
we
developed
an
integrated
box
instant
nucleic
screening
which
fully
integrates
release,
amplification,
results
visualization
self-service
standalone
test.
Importantly,
operation
runs
on
novel
gamepad-like
interface,
allows
deployment
in
home
by
users
without
any
prior
professional
training.
The
performance
is
empowered
RNA
extraction-free
sample
inactivation
process
nested
recombinase
polymerase
amplification
chemistry
exhibits
sensitivity
comparable
reverse
transcription-quantitative
chain
reaction
with
high
specificity
severe
acute
respiratory
syndrome
coronavirus
2
time
30
minutes
directly
from
fresh
swab
results.
These
innovations
make
platform
convenient,
accurate,
deployable
point-of-care
testing
scheme.
Sustainable Food Technology,
Journal Year:
2024,
Volume and Issue:
2(4), P. 976 - 992
Published: Jan. 1, 2024
The
integration
of
advanced
biosensors
enhances
the
detection
contaminants
in
food.
This
approach
addresses
challenges
related
to
sensitivity,
specificity,
and
environmental
factors,
ensuring
food
safety
quality.
Sensors and Actuators A Physical,
Journal Year:
2024,
Volume and Issue:
376, P. 115625 - 115625
Published: June 24, 2024
The
integration
of
CRISPR
technology
with
microfluidic-based
biosensors
has
greatly
expanded
its
applications
in
medicine
and
molecular
biology.
This
combination
offers
enhanced
sensitivity
selectivity
medical
diagnostics.
CRISPR-powered
microfluidics
enables
the
accurate
quantification
DNA
RNA
associated
diseases
such
as
cancer,
viral
infections,
bacterial
diseases.
precise
targeting
capabilities
allow
for
detection
specific
genetic
sequences,
aiding
early
diagnosis,
disease
monitoring,
treatment
assessment.
improves
specificity
by
leveraging
programmable
nature
coupling
it
signal
readouts.
However,
challenges
assay
optimization,
standardization,
device
fabrication
need
to
be
addressed.
Additionally,
complexity
presents
potential
limitations
terms
off-target
effects
unintended
modifications.
Overall,
holds
tremendous
diagnostics,
but
further
research
development
are
required
fully
exploit
benefits
clinical
settings.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(2), P. 249 - 249
Published: Feb. 9, 2023
The
detection
of
pathogens
in
food
substances
is
crucial
concern
for
public
health
and
the
safety
natural
environment.
Nanomaterials,
with
their
high
sensitivity
selectivity
have
an
edge
over
conventional
organic
dyes
fluorescent-based
methods.
Advances
microfluidic
technology
biosensors
taken
place
to
meet
user
criteria
sensitive,
inexpensive,
user-friendly,
quick
detection.
In
this
review,
we
summarized
use
fluorescence-based
nanomaterials
latest
research
approaches
towards
integrated
biosensors,
including
microsystems
containing
detection,
various
model
systems
nano
materials,
DNA
probes,
antibodies.
Paper-based
lateral-flow
test
strips
microchips
as
well
most-used
trapping
components
are
also
reviewed,
possibility
performance
portable
devices
evaluated.
We
present
a
current
market-available
system
which
was
developed
screening
highlight
future
direction
development
on-site
stratification
common
foodborne
pathogens.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: April 9, 2025
Avian
influenza
outbreaks
have
had
significant
economic
and
public
health
consequences
worldwide.
Therefore,
prompt,
reliable,
cost-effective
diagnostic
devices
are
crucial
for
scrutinizing
confining
highly
pathogenic
avian
viruses
(HPAIVs).
Our
study
introduced
evaluated
a
novel
paper-based
loop-mediated
isothermal
amplification
(LAMP)
test
diagnosing
the
H5
subtype
of
virus
(AIV).
We
meticulously
designed
screened
LAMP
primers
targeting
H5-haemagglutinin
(H5-HA)
gene
AIV
fine-tuned
detection
assay
best
performance.
The
demonstrated
limit
500
copies
per
reaction
(25
copies/µl).
Additionally,
exhibited
no
cross-reactivity
with
common
bovine
pathogens,
confirming
its
specificity.
Spiking
experiments
revealed
that
could
accurately
detect
1000
synthetic
HPAIV
RNA
(per
reaction)
when
spiked
into
oropharyngeal
swab
samples,
achieving
100%
analytical
sensitivity,
specificity,
accuracy.
This
inexpensive,
user-friendly
point-of-need
tool
holds
great
promise,
especially
in
resource-limited
settings.
It
only
requires
water
bath
incubation
enables
visual
results
without
special
equipment.
Overall,
provides
promising
method
rapidly
reliably
detecting
AIV,
contributing
to
improved
surveillance
early
intervention
strategies.
Biosensors and Bioelectronics,
Journal Year:
2024,
Volume and Issue:
259, P. 116374 - 116374
Published: May 10, 2024
Laboratory-based
nucleic
acid
amplification
tests
(NAATs)
are
highly
sensitive
and
specific,
but
they
require
the
transportation
of
samples
to
centralized
testing
facilities
have
long
turnaround
times.
During
Coronavirus
Disease
2019
(COVID-19)
pandemic,
substantial
advancement
has
been
achieved
with
development
paper-based
point-of-care
(POC)
NAATs,
offering
features
such
as
low
cost,
being
easy
use,
providing
rapid
sample-to-answer
Although
most
POC
NAATs
innovations
towards
clinical
settings,
we
developed
a
portable,
loop-mediated
isothermal
(LAMP)
platform
for
on-farm
applications,
capable
detecting
Bacteroidales
fecal
contamination
biomarker.
Our
integrated
includes
drop
generator,
heating
imaging
unit,
biosensors,
results
(limit
detection
3
copies
per
cm
Lab on a Chip,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
A
post-pandemic
perspective
on
paper-based
microfluidics
by
George
Whitesides
and
collaborators.
Sketch
generated
artificial
intelligence
using
an
original
photograph
courtesy
of
Volker
Steger
(Science
Photo
Library).
Molecules,
Journal Year:
2024,
Volume and Issue:
29(14), P. 3322 - 3322
Published: July 15, 2024
Pathogenic
microorganisms
play
a
crucial
role
in
the
global
disease
burden
due
to
their
ability
cause
various
diseases
and
spread
through
multiple
transmission
routes.
Immunity
tests
identify
antigens
related
these
pathogens,
thereby
confirming
past
infections
monitoring
host's
immune
response.
Traditional
pathogen
detection
methods,
including
enzyme-linked
immunosorbent
assays
(ELISAs)
chemiluminescent
immunoassays
(CLIAs),
are
often
labor-intensive,
slow,
reliant
on
sophisticated
equipment
skilled
personnel,
which
can
be
limiting
resource-poor
settings.
In
contrast,
development
of
microfluidic
technologies
presents
promising
alternative,
offering
automation,
miniaturization,
cost
efficiency.
These
advanced
methods
poised
replace
traditional
by
streamlining
processes
enabling
rapid,
high-throughput
immunity
testing
for
pathogens.
This
review
highlights
latest
advancements
systems
designed
rapid
testing,
incorporating
immunosensors,
single
molecule
arrays
(Simoas),
lateral
flow
assay
(LFA),
smartphone
integration.
It
focuses
key
pathogenic
such
as
SARS-CoV-2,
influenza,
ZIKA
virus
(ZIKV).
Additionally,
discusses
challenges,
commercialization
prospects,
future
directions
advance
infectious
detection.
Animals,
Journal Year:
2023,
Volume and Issue:
13(3), P. 524 - 524
Published: Feb. 2, 2023
COVID-19
is
one
of
the
deadliest
epidemics.
This
pandemic
caused
by
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
but
role
dogs
in
spreading
disease
human
society
poorly
understood.
review
sheds
light
on
limited
susceptibility
to
infections
which
likely
attributed
relatively
low
levels
angiotensin-converting
enzyme
(ACE2)
tract
and
phylogenetic
distance
ACE2
from
receptor.
The
affect
binding
affinity
between
spike
proteins
resulting
it
being
uncommon
for
spread
disease.
To
demonstrate
COVID-19,
we
reviewed
epidemiological
studies
prevalence
SARS-CoV-2
dogs.
Additionally,
discussed
use
detection
as
a
rapid
reliable
method
effectively
discriminating
infected
non-infected
individuals
using
different
types
samples
(secretions,
saliva,
sweat).
We
considered
available
information
human–dog
interfaces
involving
possibility
transmission
vice
versa,
behavior
changes,
importance
preventive
measures
because
risk
domestic
remains
concern.