Frontiers in Microbiology,
Journal Year:
2022,
Volume and Issue:
13
Published: Dec. 9, 2022
Coronavirus
disease
2019
(COVID-19)
is
caused
by
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
and
has
brought
a
huge
threat
to
public
health
the
global
economy.
Rapid
identification
isolation
of
SARS-CoV-2-infected
individuals
are
regarded
as
one
most
effective
measures
control
pandemic.
Because
its
high
sensitivity
specificity,
nucleic
acid
testing
become
major
method
SARS-CoV-2
detection.
A
deep
understanding
different
diagnosis
methods
for
COVID-19
could
help
researchers
make
an
optimal
choice
in
detecting
at
symptom
stages.
In
this
review,
we
summarize
evaluate
latest
developments
current
detection
SARS-CoV-2.
particular,
discuss
biosensors
CRISPR-based
diagnostic
systems
their
characteristics
challenges.
Furthermore,
emerging
variants
impact
on
systematically
introduced
discussed.
Considering
dynamics,
also
recommend
optional
tests
From
sample
preparation
results
readout,
conclude
pointing
out
pain
points
future
directions
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 3, 2025
Development
of
a
multiplexed
and
sensitive
biosensing
platform
is
priority
for
public
health
security.
We
report
micropore
resistance
counting
based
on
polystyrene
microsphere
size-based
encoding
Clostridium
butyricum
Argonaute
(CbAgo)
decoding
ultrasensitive
detection.
Initially,
we
constructed
target
DNA-modified
coding
system
counting.
Subsequently,
the
precise
recognition
cleavage
capabilities
guide
DNA-activated
CbAgo
protein
enable
encoded
system.
Changes
in
concentration
microspheres
are
presented
as
signal
readout.
The
demonstrated
excellent
performance
detection
three
mycotoxins
(with
sensitivity
range
over
4
orders
magnitude
reaching
pg/mL
level)
two
inflammatory
markers
at
pg/mL.
Combining
enzyme
by
with
counting,
developed
highly
tool
wide-ranging
potential
applications
such
clinical
diagnosis,
food
safety
inspection,
environmental
monitoring.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(10), P. 3623 - 3642
Published: Oct. 11, 2023
Over
the
past
few
decades,
pathogens
have
posed
a
threat
to
human
security,
and
rapid
identification
of
should
be
one
ideal
methods
prevent
major
public
health
security
outbreaks.
Therefore,
there
is
an
urgent
need
for
highly
sensitive
specific
approaches
identify
quantify
pathogens.
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
CRISPR/Cas
systems
Argonaute
(Ago)
belong
Microbial
Defense
Systems
(MDS).
The
guided,
programmable,
targeted
activation
nucleases
by
both
them
leading
way
new
generation
detection.
We
compare
these
two
in
terms
similarities
differences.
In
addition,
we
discuss
future
challenges
prospects
development
biosensors,
especially
electrochemical
biosensors.
This
review
expected
afford
researchers
entering
this
multidisciplinary
field
useful
guidance
provide
inspiration
more
innovative
biosensors
