Real-time quantification of proteins secreted of conditioned media from mesenchymal stromal cells (MSC) in co-culture with hematopoietic progenitor cells DOI Creative Commons

Pamela Quimbaya,

Vivian Garzón,

Rosa-Helena Bustos

et al.

Sensing and Bio-Sensing Research, Journal Year: 2023, Volume and Issue: 43, P. 100608 - 100608

Published: Nov. 28, 2023

The interaction of mesenchymal stromal cells (MSC) in co-cultures with hematopoietic progenitor (HPC) ex-vivo models induce the secretion pro-inflammatory protein factors and influences HPC cell proliferation differentiation processes. Different studies have shown quantification these biomarkers other conditioned media using an ELISA Luminex™ type immunoassay format. In this paper, we developed real-time from bone marrow, umbilical cord blood, Wharton's jelly sources surface plasmon resonance imaging (SPRi). We show detection CCL2, CCL5, bFGF, IL-6, IL-8 VEGF proteins high sensitivity specificity. SPRi can be used a analysis methodology could considered tool for biomarker assays.

Language: Английский

Label-free integrated microfluidic plasmonic biosensor from vertical-cavity surface-emitting lasers for SARS-CoV-2 receptor binding domain protein detection DOI Creative Commons

Wenhui Jiang,

Zhengtai Ma,

Fang Cao

et al.

Optics Express, Journal Year: 2023, Volume and Issue: 31(8), P. 12138 - 12138

Published: March 17, 2023

The nanoplasmonic sensor of the nanograting array has a remarkable ability in label-free and rapid biological detection. integration with standard vertical-cavity surface-emitting lasers (VCSEL) platform can achieve compact powerful solution to provide on-chip light sources for biosensing applications. Here, high sensitivity integrated VCSELs was developed as suitable analysis technique COVID-19 specific receptor binding domain (RBD) protein. gold is on realize microfluidic plasmonic biosensor biosensing. 850 nm are used source excite localized surface plasmon resonance (LSPR) effect detect concentration attachments. refractive index 2.99 × 106 nW/RIU. aptamer RBD modified protein successfully. wide detection range 0.50 ng/mL - 50 µg/mL. This provides an integrated, portable, miniaturized idea biomarker

Language: Английский

Citations

5

All-fiber biological detection microfluidic chip based on space division and wavelength division multiplexing technologies DOI
Yong Wei,

Zhuo Ren,

Chunlan Liu

et al.

Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(23), P. 4501 - 4510

Published: Jan. 1, 2022

To further reduce the size of a microfluidic detection chip and sample consumption to shorten manufacturing cycle, flexible compact all-fiber multichannel SPR was proposed demonstrated in this paper.

Language: Английский

Citations

8

The motion of micro-swimmers over a cavity in a micro-channel DOI
Xiao Hu, Weijin Chen, Jianzhong Lin

et al.

Soft Matter, Journal Year: 2024, Volume and Issue: 20(12), P. 2789 - 2803

Published: Jan. 1, 2024

This article combines the lattice Boltzmann method (LBM) with squirmer model to investigate motion of micro-swimmers in a channel-cavity system.

Language: Английский

Citations

1

Evaluating bioreceptor immobilization on Gold Nanospike (AuNS)–modified Screen-Printed Carbon Electrode (SPCE) as enzymatic glucose biosensor DOI Creative Commons

Salma Majidah,

Lavita Nuraviana Rizalputri,

Eduardus Ariasena

et al.

Nanocomposites, Journal Year: 2024, Volume and Issue: 10(1), P. 125 - 137

Published: April 3, 2024

Integration of gold nanoparticles onto electrochemical biosensor electrodes has been widely conducted to improve the performance biosensors. Gold nanospikes (AuNS), as one nanoparticle morphologies, can be integrated into biosensors through electrodeposition and potential immobilize bioreceptor on using self-assembled monolayer (SAM) method. This paper examines AuNS-deposited Screen-Printed Carbon Electrodes (SPCEs) immobilizing enzymes label-based by evaluating optimum parameter for glucose oxidase (GOx) enzyme immobilization SPCE that consists incubation time concentration SAM molecule—L-cysteine—and GOx enzyme, then reviews its performances. The developed exhibits excellent in detecting (linear range 0.2–15 mM limit detection (LOD) 116 µM), with good selectivity against uric acid, urea, ascorbic dopamine, lactic superiority towards nanosphere modified biosensor.

Language: Английский

Citations

1

Endorsing a Hidden Plasmonic Mode for Enhancement of LSPR Sensing Performance in Evolved Metal–insulator Geometry Using an Unsupervised Machine Learning Algorithm DOI Creative Commons
Nikhil Bhalla, Atul Thakur, I. S. Édelman

et al.

ACS Physical Chemistry Au, Journal Year: 2022, Volume and Issue: 2(6), P. 459 - 467

Published: Sept. 1, 2022

Large-area nanoplasmonic structures with pillared metal–insulator geometry, also called nanomushrooms (NM), consist of an active spherical-shaped plasmonic material such as gold its cap and silicon dioxide stem. NM is a geometry which evolves from precursor, nanoislands (NI) consisting aforementioned spherical on flat substrates, via selective physical or chemical etching the dioxide. The well-known to provide enhanced localized surface plasmon resonance (LSPR) sensitivity in biosensing applications compared NI. However, precise optical phenomenon behind this enhancement unknown often associated existence electric fields large fraction spatial region between pillars NM, usually accessible by biomolecules. Here, we uncover association LSPR geometries hidden mode conducting magneto-optics measurements deconvoluting absorbance spectra obtained during local refractive index change NI geometries. By virtue principal component analysis, unsupervised machine learning technique, observe explicit relationship deconvoluted modes LSPR, differential absorption left right circular polarized light, sensor. Our findings may lead development new approaches extract properties materials establish fundamental relationships less understood photonic nanomaterials.

Language: Английский

Citations

6

Analysis of the Correlation between the Mental Stress of College Students and Their Learning Initiative during the Outbreak of COVID-19 DOI Open Access

Limin Xi,

Bin Wen,

Hua Zhao

et al.

International Journal of Emerging Technologies in Learning (iJET), Journal Year: 2022, Volume and Issue: 17(23), P. 145 - 159

Published: Dec. 8, 2022

The spread of COVID-19 has brought negative impacts on the life and study college students thus aggravated their mental stress in this environment. Existing research focused more status quo problem while having ignored its process variability, there is no correlation between learning initiative during outbreak COVID-19. To end, paper conducts an analysis In-depth was carried out by factor method SPSS, evaluation model built for based four stressors, with how to obtain results given. grey used quantitatively analyze COVID-19, that is, factors affecting students’ were investigated through analysis. Finally, relevant experimental

Language: Английский

Citations

1

Real-time quantification of proteins secreted of conditioned media from mesenchymal stromal cells (MSC) in co-culture with hematopoietic progenitor cells DOI Creative Commons

Pamela Quimbaya,

Vivian Garzón,

Rosa-Helena Bustos

et al.

Sensing and Bio-Sensing Research, Journal Year: 2023, Volume and Issue: 43, P. 100608 - 100608

Published: Nov. 28, 2023

The interaction of mesenchymal stromal cells (MSC) in co-cultures with hematopoietic progenitor (HPC) ex-vivo models induce the secretion pro-inflammatory protein factors and influences HPC cell proliferation differentiation processes. Different studies have shown quantification these biomarkers other conditioned media using an ELISA Luminex™ type immunoassay format. In this paper, we developed real-time from bone marrow, umbilical cord blood, Wharton's jelly sources surface plasmon resonance imaging (SPRi). We show detection CCL2, CCL5, bFGF, IL-6, IL-8 VEGF proteins high sensitivity specificity. SPRi can be used a analysis methodology could considered tool for biomarker assays.

Language: Английский

Citations

0