Qualitative and quantitative detection and identification of two benzodiazepines based on SERS and convolutional neural network technology DOI
Xuanyu Sha, Guoqiang Fang, Guangxu Cao

et al.

The Analyst, Journal Year: 2022, Volume and Issue: 147(24), P. 5785 - 5795

Published: Jan. 1, 2022

Drug abuse is a global social issue of concern. As the drug market expands, there an urgent need for technological methods to rapidly detect meet needs different situations. Here, we present strategy rapid identification benzodiazepines (midazolam and diazepam) using surface-enhanced Raman scattering (SERS) combined with neural networks (CNN). The method uses self-assembled silver nanoparticle paper-based SERS substrate detection. Then, spectrum intelligent recognition model based on deep learning technology was constructed realize sensitive distinction between two drugs. In this work, total 560 spectra were collected, qualitative quantitative drugs in water beverage (Sprite) realized by trained convolutional network predicted concentrations each scenario could reach 0.1-50 ppm water), 0.5-50 diazepam Sprite), 5-150 (diazepam strong coefficient determination (R2) larger than 0.9662. advantage that can extract data features from entire spectrum, which makes up information loss when manually identifying selecting limited number characteristic peaks. This work clearly clarifies combination has become inevitable development trend, also demonstrates great potential practical application SERS.

Language: Английский

Plasmonic nanoparticle sensors: current progress, challenges, and future prospects DOI Creative Commons
Krishna Kant, Reshma Beeram, Yi Cao

et al.

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(12), P. 2085 - 2166

Published: Jan. 1, 2024

This comprehensive review summarizes the past, present, and future of plasmonic NP-based sensors in terms different sensing platforms, chemical biological analytes, expected technologies.

Language: Английский

Citations

18
Using a crumpled graphene field-effect transistor for ultrasensitive SARS-CoV-2 detection via its papain-like protease DOI
Shicai Xu,

Meng Tian,

Chonghui Li

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 505, P. 159386 - 159386

Published: Jan. 8, 2025

Language: Английский

Citations

2
Rapid Detection of SARS-CoV-2 RNA in Human Nasopharyngeal Specimens Using Surface-Enhanced Raman Spectroscopy and Deep Learning Algorithms DOI
Yanjun Yang, Hao Li,

Les Jones

et al.

ACS Sensors, Journal Year: 2022, Volume and Issue: 8(1), P. 297 - 307

Published: Dec. 23, 2022

A rapid and cost-effective method to detect the infection of SARS-CoV-2 is fundamental mitigating current COVID-19 pandemic. Herein, a surface-enhanced Raman spectroscopy (SERS) sensor with deep learning algorithm has been developed for detection RNA in human nasopharyngeal swab (HNS) specimens. The SERS was prepared using silver nanorod array (AgNR) substrate by assembling DNA probes capture RNA. spectra HNS specimens were collected after hybridization, corresponding peaks identified. range determined be 103-109 copies/mL saline sodium citrate buffer. recurrent neural network (RNN)-based model classify 40 positive 120 negative an overall accuracy 98.9%. For blind test 72 specimens, RNN gave 97.2% prediction 100% All detections performed 25 min. These results suggest that DNA-functionalized AgNR combined could serve as potential point-of-care diagnostic platform.

Language: Английский

Citations

38
Nanotechnology-Assisted Biosensors for the Detection of Viral Nucleic Acids: An Overview DOI Creative Commons
Hye Kyu Choi, Jinho Yoon

Biosensors, Journal Year: 2023, Volume and Issue: 13(2), P. 208 - 208

Published: Jan. 30, 2023

The accurate and rapid diagnosis of viral diseases has garnered increasing attention in the field biosensors. development highly sensitive, selective, accessible biosensors is crucial for early disease detection preventing mortality. However, developing optimized several limitations, including mutations. For decades, nanotechnology been applied numerous biological fields such as biosensors, bioelectronics, regenerative medicine. Nanotechnology offers a promising strategy to address current limitations conventional nucleic acid-based implementation nanotechnologies, functional nanomaterials, nanoplatform-fabrication techniques, surface nanoengineering, not only improved performance but also expanded range sensing targets. Therefore, deep understanding combination nanotechnologies required prepare sanitary emergencies recent COVID-19 pandemic. In this review, we provide interdisciplinary information on nanotechnology-assisted First, representative are discussed, after which review summarizes various acid expect that will valuable basis novel

Language: Английский

Citations

22
High Accuracy of Clinical Verification of Electrohydrodynamic-Driven Nanobox-on-Mirror Platform for Molecular Identification of Respiratory Viruses DOI
Junrong Li, Rui Guan, Alain Wuethrich

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(11), P. 4495 - 4504

Published: March 6, 2024

The molecular detection of multiple respiratory viruses provides evidence for the rational use drugs and effective health management. Herein, we developed tested clinical performance an electrohydrodynamic-driven nanobox-on-mirror platform (E-NoM) parallel, accurate, sensitive four viral antigens. E-NoM uses gold–silver alloy nanoboxes as core material with deposition a silver layer shell on surfaces to amplify enable reproducible Raman signal readout that facilitates accurate detection. Additionally, employs gold microelectrode arrays mirror electrohydrodynamics manipulate fluid flow enhance interactions improved biosensing response. presence antigens binds nanobox-based core–shell nanostructure creates nanocavity extremely strong "hot spots" benefit analysis. Significantly, in large cohort 227 patients, designed demonstrates capability screening infection achieved specificity, sensitivity, accuracy 100.0, 96.48, 96.91%, respectively. It is anticipated can find position usage disease diagnosis.

Language: Английский

Citations

14
Factors that Affect Quantification in Surface-Enhanced Raman Scattering DOI
Meikun Fan, Alexandre G. Brolo

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Surface-enhanced Raman scattering (SERS) is an analytical technique capable of detecting trace amounts specific species. The uniqueness vibrational signatures a major advantage SERS. This combination sensitivity and specificity has motivated researchers to develop diverse methodologies leveraging However, even 50 years after its first observation, SERS still perceived as unreliable for quantification. perception precluded the application in laboratories that rely on consistent quantification (for regulatory purposes, instance). In this review, we describe some aspects lead intensity variations how those challenges were addressed technique. goal identify sources intensities then demonstrate that, with these pitfalls, can be used when factors such nature substrate, experimental conditions, sample preparation, surface chemistry, data analysis are carefully considered tailored particular application.

Language: Английский

Citations

1
Construction of histamine aptamer sensor based on Au NPs nanozyme for ultrasensitive SERS detection of histamine DOI
Baihui Wang, Huan Jiang,

Ruyi Tang

et al.

Journal of Food Composition and Analysis, Journal Year: 2023, Volume and Issue: 120, P. 105337 - 105337

Published: April 14, 2023

Language: Английский

Citations

20
Bilateral efforts to improve SERS detection efficiency of exosomes by Au/Na7PMo11O39 Combined with Phospholipid Epitope Imprinting DOI

Qingnan Zhao,

Xianhui Cheng,

Saizhen Hu

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 258, P. 116349 - 116349

Published: April 29, 2024

Language: Английский

Citations

7
SERS/EC Dual-Mode Sensing System for the Assay of SARS-CoV-2 RNA by Using Single Gold Nanowires as a Platform DOI

Qingshan Dai,

Jiarui Huang, Xia Qiu

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(6), P. 6369 - 6379

Published: March 12, 2024

The continuous spread and infection of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) worldwide has brought a great impact on people's lives health become serious public problem. In this contribution, surface-enhanced Raman scattering/electrochemical (SERS/EC) dual-mode sensing system using single Au nanowires (Au NWs) as platform is proposed for detecting SARS-CoV-2 RNA with excellent sensitivity selectivity. NWs modified bimetallic NPs can significantly increase SERS/EC signals, presence produce attenuated signals by shearing off aptamer containing signal molecules. addition to its specific recognition high (detection limits 1.02 pM SERS method 0.21 EC method), nanosensor been successfully applied samples complex matrices expected serve potential, sensitive, reliable tool virus screening protein detection, especially in vivo detection due small overall dimension NWs.

Language: Английский

Citations

5
CRISPR/Cas13a Trans-Cleavage and Catalytic Hairpin Assembly Cascaded Signal Amplification Powered SERS Aptasensor for Ultrasensitive Detection of Gastric Cancer-Derived Exosomes DOI
Xinyue Gu, Jingjing Zhang,

Jing Liang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Cancer-derived exosomes carry a large number of specific molecular profiles from cancer cells and have emerged as ideal biomarkers for early diagnosis. Accurate detection ultralow-abundance in complex biological samples remains great challenge. Herein, novel SERS aptasensor powered by cascaded signal amplification CRISPR/Cas13a

Language: Английский

Citations

5