Journal of Industrial and Engineering Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(4), P. 1651 - 1658
Published: Jan. 19, 2024
The development of a highly accurate electrochemiluminescence (ECL) signal switch to avoid nonspecific stimulus responses is currently significant and challenging task. Here, we constructed universal utilizing luminophore–quencher pair mesostructured silica xerogel-confined polymer gold nanoparticles (Au NPs) that can accurately detect low-abundance epigenetic markers in complex sample systems. Notably, the ECL encapsulated xerogel acts as luminophore, which demonstrated specific dependence on Au NPs-mediated energy transfer quenching. To demonstrate feasibility, specifically labeled 5-hydroxymethylcytosine (5hmC) site random sequence using double-stranded (dsDNA) tag was skillfully designed with CRISPR/Cas12a activator recombinant polymerase amplification (RPA) template. After by RPA, large amount dsDNA generated initiate trans-cleavage activity subsequently activate switch, allowing for precise quantification 5hmC. improves stability luminophore prevents responses, providing new paradigm constructing high-precision biosensors.
Language: Английский
Citations
11
Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 401, P. 135024 - 135024
Published: Nov. 20, 2023
Language: Английский
Citations
20
Frontiers in Chemical Biology, Journal Year: 2024, Volume and Issue: 2
Published: Jan. 10, 2024
Porphyrin complexes are present in many natural systems and have significant biological roles, such as light harvesting, oxygen transport, catalysis. Owing to their intrinsic aromatic structure, porphyrin derivatives exhibit characteristic photophysical electrochemical properties. Porphyrins porphyrin-based been extensively utilized biomedical applications during the last decade. Specifically, porphyrinoids tested agents antimicrobial photodynamic therapy, well imaging (e.g., diagnosis of cancer cells). This perspective article summarizes recent developments our group concerning application applications. The current challenges future prospects exploitation materials also discussed.
Language: Английский
Citations
8
Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(6)
Published: May 31, 2024
Electrogenerated chemiluminescence (ECL) stands out as a remarkable phenomenon of light emission at electrodes initiated by electrogenerated species in solution. Characterized its exceptional sensitivity and minimal background optical signals, ECL finds applications across diverse domains, including biosensing, imaging, various analytical applications. This review aims to serve comprehensive guide the utilization Beginning with brief exposition on theory basis generation, we elucidate systems employed initiate ECL. Furthermore, delineate principal utilized for generation contexts, elucidating both advantages challenges inherent their use. Additionally, provide an overview different electrode materials novel ECL-based protocols tailored purposes, specific emphasis biosensing
Language: Английский
Citations
8
Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 153, P. 104706 - 104706
Published: Sept. 10, 2024
Language: Английский
Citations
8
Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(5), P. 1355 - 1360
Published: Jan. 1, 2024
Gold nanoclusters (Au NCs) with excellent optical properties and biocompatibility have become one of the most promising electrochemiluminescence (ECL) emitters. However, low efficiency poor stability Au NCs restrict their applications in ECL. Herein, by supramolecular assembly L-arginine (Arg) 4-hydroxy-2-mercapto-6-methylpyrimidine (MTU) on surface NCs, Arg/MTU-Au enhanced ECL were prepared. Compared MTU-stabilized (MTU-Au NCs), increased 24.8 times. As a proof-of-concept, sensitive biosensing platform was constructed for detection hemoglobin (Hb) urine using as The proposed provides feasible strategy biomarkers has broad application prospects disease screening clinical marker detection.
Language: Английский
Citations
7
Electrochimica Acta, Journal Year: 2024, Volume and Issue: 489, P. 144256 - 144256
Published: April 9, 2024
Electrochemiluminescence (ECL), an intriguing luminescent phenomenon induced by electrochemical stimulation, has evolved from studying electron transfer reactions to a powerful analytical method and imaging technique. ECL can be generated through annihilation or co-reactant methods, with recent advancements integrating it into devices for diverse applications. This review traces the evolution of its early applications developments in technology. Notably, utilization charge-coupled (CCD) multiplying (EMCCD) microscopy revolutionized capabilities, making cost-effective option point-of-care testing. The explores heterogeneous mechanism, emphasizing limitations challenges visualizing objects away electrode surface. synergy between is highlighted, showcasing contributions understanding mechanisms improving use biological contexts. Finally, encapsulates innovative approaches material design, surface modification, architecture, providing comprehensive overview strategies control active layer advance microscopy.
Language: Английский
Citations
7
Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 113263 - 113263
Published: June 5, 2024
Language: Английский
Citations
7
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 21, 2025
In marine environmental monitoring, due to the presence of a large number interfering proteins and bacteria in seawater, it is great significance construct an efficient sensing interface with antifouling antibacterial functions avoid aforementioned interferences. On this basis, zwitterionic hydrogel based on sulfobetaine methacrylate (SBMA) bovine serum albumin (BSA) was developed as coating. The combination hydration zwitterions hydrophilicity hydrogels endows BSA@PSBMA good antiadsorption ability, which effectively hinders adhesion bacteria, thereby improving detection sensitivity biosensor. At same time, covalent grafting SBMA BSA solves defect poor stability individual complex matrices, service life addition, Au@luminol encapsulated internal standard realize one-step integration ratio strategies, simplifies construction process electrochemiluminescence biosensor exhibited accuracy for chloramphenicol detection, wide linear range 1 pM 100 nM low limit 0.39 (S/N = 3), suitable trace antibiotics seawater.
Language: Английский
Citations
1
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160268 - 160268
Published: Feb. 1, 2025
Language: Английский
Citations
1