Dual-site responsive module-triggered CRISPR/Cas12a switch with enhanced reaction kinetics for specific detection of PSA DOI
Wenjing Yang, Yi Li, Meiling Liu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157912 - 157912

Published: Nov. 1, 2024

Language: Английский

CRISPR/Cas13a-Programmed Cu NCs and Z-Scheme T-COF/Ag2S for Photoelectrochemical Biosensing of circRNA DOI
Xinxin Yuan, Wen‐Chao Geng,

Jiangying Ji

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Circular RNAs (circRNAs), as a class of noncoding RNA molecules with circular structure exhibit high stability and spatiotemporal-specific expression, making them ideal cancer biomarkers for liquid biopsy. Herein, new photoelectrochemical (PEC) biosensor highly sensitive circRNA assay in the whole blood lung patients was designed based on CRISPR/Cas13a-programmed Cu nanoclusters (Cu NCs) Z-scheme covalent organic framework/silver sulfide (T-COF/Ag2S) composite. This T-COF/Ag2S composite accelerates electron transfer produces an excellent initial photocurrent. When CRISPR/Cas13a precisely targets circRNA, it nonspecifically cleaves triple-helix molecular to release DNA fragments (C'/C"). After C'/C" opens hairpin probe (HP) modified electrode, hybridization chain reactions are performed produce abundant AT-rich double-stranded addition H1 H2 probes. Upon incubation Cu2+, NCs situ formed via A-Cu2+-T bonds can effectively quench photocurrent due energy process. developed PEC shows low limit detection 0.5 fM, reusability DNA-modified magnetic beads (MB-DNA) reduces cost. Moreover, accurately quantify level distinguish expression from healthy controls patients, offering strong potential clinical diagnosis.

Language: Английский

Citations

2
CRISPR/Cas12a-drived electrochemiluminescence and fluorescence dual-mode magnetic biosensor for sensitive detection of Pseudomonas aeruginosa based on iridium(III) complex as luminophore DOI

Yaoyao Xu,

Jingjing Ma,

Chenji Dai

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116678 - 116678

Published: Aug. 15, 2024

Language: Английский

Citations

9
Supramolecular DNA Nanodevice Assembled via RCA and HCR Cascade Reaction on Tetrahedral DNA Nanostructure for Sensitive Detection and Intracellular Imaging of Dual-miRNAs Associated with Liver Cancer DOI

Xue-Mei Zhou,

Si–Yi Chen, Yaqin Chai

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade on tetrahedral nanostructure (TDN) to achieve simultaneous sensitive detection intracellular imaging of dual-miRNAs related liver cancer. The effectively addressed limitations low probe loading capacity in traditional TDN nanodevices by enriching plenty signal probes around single TDN, significantly enhancing fluorescence signal. Impressively, with fulcrum dense structure imparted strong rigidity, ensuring stability decrease aggregation quenching for further increasing response. Consequently, biosensor based enabled miRNA221 miRNA222, achieved accurate situ displaying improved capabilities compared TDN-based nanodevices. More importantly, precise miRNA222 could distinguish hepatocellular carcinoma cells different degrees metastasis from human normal cells, providing more information diagnosis development carcinoma.

Language: Английский

Citations

1
Wavelength-Resolved Magnetic Multiplex Biosensor for Simultaneous and Ultrasensitive Detection of Pneumonia Pathogens via Catalytic Hairpin Assembly Strategy with Luminescent Iridium Complexes DOI

Libing Ke,

Chenji Dai,

Yaoyao Xu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Pneumonia is a prevalent acute respiratory infection and major cause of mortality hospitalization, the urgent demand for rapid, direct, highly accurate diagnostic method capable detecting both Streptococcus pneumoniae (S. pneumoniae) Klebsiella (K. arises from their prominent roles as primary pathogens responsible pneumonia. Herein, two luminescent iridium complexes with nonoverlapping photoluminescence spectra, iridium(III)-bis [4,6-(difluorophenyl)-pyridinato-N,C2′] picolinate (abbreviated Ir–B) bis (2-(3,5- dimethylphenyl) quinoline-C2,N′) (acetylacetonato) iridium(III)) Ir-R), were unprecedently proposed to construct novel wavelength-resolved magnetic multiplex biosensor simultaneous detection S. K. based on catalytic hairpin assembly (CHA) signal amplification strategy combined dye-doped silica nanoparticles. Notably, not only exhibits broad linear range 50 pM 10 nM but also demonstrates excellent recovery rates (96.1–99.3%) (94.8–101.5%) in real clinical samples, corresponding relative standard deviation (RSD) values ranging 2.57 3.15% 1.45 3.17% pneumoniae. These favorable experimental outcomes undoubtedly offer promising approach multiple future.

Language: Английский

Citations

0
Self-enhanced Biosensor with Luminophore-catalyst Integrated Strategies and ECL-RET for Ultrasensitive SARS-CoV-2 N Immunoassay DOI
Liang Kong, Lu Zhao, Huixin Liang

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137478 - 137478

Published: Feb. 1, 2025

Language: Английский

Citations

0
Exploring a New Ruthenium(II) Complex with High DNA Binding Ability as a Novel Efficient Luminescent Intercalation Agent to Construct a Label-Free ECL/PL Dual-Mode Biosensor DOI

Yaoyao Xu,

Rui Deng, Junli Jia

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Ruthenium(II) complexes with special ligands have been widely recognized in numerous fields and attributed to their outstanding DNA binding capacity. Hybridization chain reaction (HCR), as an enzyme-free amplification technique, forms long double-stranded (dsDNA) structures, which provides intercalation platform for these obtains effective enhancement of luminescent signals a significant extent enhances the sensitivity detection. Hence, Ru(dip)2(tpphz) [dip = 4,7-diphenyl-1,10-phenanthroline, tpphz tetrapyrido[3,2-a:2′,3′-c:3″,2″-h:2‴,3‴-j]phenazine] confirmed possess high capacity via UV–vis absorption spectroscopy AutoDock theoretical simulation calculations was synthesized luminescence probe. As proof concept, label-free ECL/PL dual-mode biosensor further constructed. In this design, magnetic silica spheres trigger were amplified by HCR hairpin DNA, forming large amounts dsDNA on surface, incorporated generate robust signals. Trigger cleaved owing activation Cas12a cleavage ability presence target, disappeared, reduced. The exhibited selectivity, LOD low 69 fM (S/N 3). results proved that has excellent ECL PL dual properties, huge potential establish biosensors simultaneously offers tremendous prospect anticancer, gene therapy, molecular probes beyond future.

Language: Английский

Citations

0
Protein-inorganic hybrid flowers with a two-stage accelerated strategy for stimulated activation of CRISPR/Cas12a enhance polynucleotide kinase biosensing DOI
Qi Wang, Xiu‐Mei Chen, Li Yang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 127981 - 127981

Published: March 21, 2025

Language: Английский

Citations

0
Ultrasensitive Electrochemiluminescence Aptamer Sensor Based on Ru@ZIF-Pd Cathode for Acetamiprid Detection DOI
Mingyue Shao,

C. W. Kong,

Yong-Fu Long

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Ru(bpy)32+ is commonly used in electrochemiluminescence (ECL) as a conventional luminophore. However, can leak off the electrode surface, resulting reduced ECL signal. In this work, nanocube composite material Ru@ZIF was prepared by incorporating doping luminescent into skeleton of Zn(II) ions and 2-methylimidazole (2-MI), probe Ru@ZIF-Pd situ growth Pd nanoparticles (Pd NPs). Potassium persulfate (K2S2O8) coreactant to investigate cathodic behavior. The ZnCoCH@MXene-Au (ZC@T-Au) accelerator promote production SO4•–, which effectively amplified intensity Ru@ZIF-Pd. Under optimal experimental conditions, we successfully constructed an aptamer sensor, achieves accurate detection acetamiprid from 0.1 fM 10 μM with limit 0.029 fM. It provides potential idea for pesticide residues food.

Language: Английский

Citations

2
Aggregation-induced electrochemiluminescence immunosensor of luminol nanocomposites for sensitive detection of CA15-3 via cascade quenching by dual enzyme active PtCu nanocages DOI
Zilong Zhang, Hai‐Yang Li, Jinglong Fang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 427, P. 137200 - 137200

Published: Dec. 31, 2024

Language: Английский

Citations

2
Dual-site responsive module-triggered CRISPR/Cas12a switch with enhanced reaction kinetics for specific detection of PSA DOI
Wenjing Yang, Yi Li, Meiling Liu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157912 - 157912

Published: Nov. 1, 2024

Language: Английский

Citations

0