Urchin-Shaped Au–Ag@Pt Sensor Integrated Lateral Flow Immunoassay for Multimodal Detection and Specific Discrimination of Clinical Multiple Bacterial Infections

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(35), P. 13101 - 13112

Published: Aug. 1, 2023

A new lateral flow immunoassay strip (LFIA) combining sensitive detection and identification of multiple bacteria remains a huge challenge. In this study, we first developed multifunctional urchin-shaped Au-Ag@Pt nanoparticles (UAA@P NPs) with unique combination colorimetric-SERS-photothermal-catalytic (CM/SERS/PT/CL) properties integrated them LFIA for multiplexed specific discrimination pathogenic in blood samples. Unlike the conventional that relied on antibody (Ab), novel introduced 4-mercaptophenylboronic acid (4-MPBA) as an ideal Ab replacer was functionalized UAA@P NPs (UAA@P/M outstanding binding enrichment capacities toward bacteria. Taking Staphylococcus aureus (S. aureus) model bacteria, limit (LOD) 3 CFU/mL SERS-LFIA, 27 PT-LFIA, 18 CL-LFIA, three which were over 330-fold, 37-fold, 55-fold more than ordinary visual CM-LFIA, respectively. Besides, SERS-LFIA is capable identifying types bacterial spiked samples (E. coli, S. aureus, P. aeruginosa) effectively according to Raman "fingerprints" by partial least-squares-discriminant analysis (PLS-DA). More importantly, successfully applied satisfactory recoveries from 90.3% 108.8% infected patients (N = 4) healthy subjects 2) great accuracy. Overall, multimodal incorporates quantitative detection, offering avenue early warning diagnosis infection.

Language: Английский

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Self-Assembly Multivalent Fluorescence-Nanobody Coupled Multifunctional Nanomaterial with Colorimetric Fluorescence and Photothermal to Enhance Immunochromatographic Assay

ACS Nano, Journal Year: 2023, Volume and Issue: 17(19), P. 19359 - 19371

Published: Oct. 2, 2023

The multimodal lateral flow immunoassay (LFIA) has provided accurate and reliable results for fast immediate detection. Nonetheless, LFIA remains challenging to develop biosensors with high sensitivity tolerance matrix interference in agro-food. In this study, we developed a self-assembled multivalent fluorescence-nanobody (Nb26-EGFP-H6) 16.5-fold 30-fold higher affinity than monovalent nanobody (Nb26). Based on the Nb26-EGFP-H6, synthesized enhanced immune-probes Zn-CN@Nb26-EGFP-H6 by pyrolyzing oxidizing an imidazolating zeolite framework-8 (ZIF-8) obtain photothermal metal–carbon nanomaterials (Zn-CN) immobilizing Nb26-EGFP-H6. rough porous structure of Zn-CN large surface area facilitates enrichment immobilization antibodies. A trimodal (tLFIA) colorimetric, fluorescent, triple signal outputs was constructed detection aflatoxin B1 (AFB1) maize. Attractively, Zn-CN-based tLFIA's multiplex guarantees sensitive AFB1, limits 0.0012 ng/mL (colorimetric signals), 0.0094 (fluorescent 0.252 (photothermal signals). trimode immunosensor 628-fold 42-fold that original Nb26-based ELISA (IC50) unimodal (LOD). This work provides idea constructing sensitive, tolerant efficient analytical platform rapidly detecting AFB1 food.

Language: Английский

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Polydopamine-coated two-dimensional nanomaterials as high-affinity photothermal signal tag towards dual-signal detection of Salmonella typhimurium by lateral flow immunoassay

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 472, P. 145110 - 145110

Published: July 29, 2023

Language: Английский

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“Four‐In‐One” Multifunctional Dandelion‐Like Gold@platinum Nanoparticles‐Driven Multimodal Lateral Flow Immunoassay

Small, Journal Year: 2024, Volume and Issue: 20(29)

Published: Feb. 16, 2024

Abstract The traditional lateral flow immunoassay (LFIA) with a single signal output mode may encounter challenges such as low sensitivity, poor detection range, and susceptibility to external interferences. These limitations hinder its ability meet the growing demand for advanced LFIA. To address these issues, rational development of multifunctional labels multimodal LFIA emerges promising strategy. Herein, this study reports using “four‐in‐one” dandelion‐like gold@platinum nanoparticles (MDGP). inherent properties MDGP, broad absorption spectrum, porous nanostructure, bimetallic composition gold platinum, endow them capacities in dual spectral‐overlapped fluorescence quenching, optical readout, catalytic activity, photothermal effect. Benefiting from their properties, MDGP‐LFIA enables outputs including fluorescent, colorimetric, signals. This allows acetamiprid at range 0.01–50 ng mL −1 , lowest qualitative quantitative results 0.5 0.008 respectively, significantly better than nanoparticles‐based diversity, complementarity, synergistic effect integrated signals improve flexibility, practicability, accuracy detection, holding great promise point‐of‐care testing platform versatile application scenarios.

Language: Английский

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Evaluation of the Multidimensional Enhanced Lateral Flow Immunoassay in Point-of-Care Nanosensors

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Point-of-care (POC) nanosensors with high screening efficiency show promise for user-friendly manipulation in the ever-increasing on-site analysis demand illness diagnosis, environmental monitoring, and food safety. Currently, inspired by merits of integrating advanced nanomaterials, molecular biology, machine learning, artificial intelligence, lateral flow immunoassay (LFIA)-based POC have been devoted to satisfying commercial demands terms sensitivity, specificity, practicality. Herein, we examine use multidimensional enhanced LFIA various fields over past two decades, focusing on introducing nanomaterials improve acquisition capability small order magnitude targets through engineering transformations emphasizing interdomain fusion collaboratively address inherent challenges current applications, such as multiplexing, development detectors quantitative analysis, more practical sensitivity enhancement. Specifically, this comprehensive review encompasses latest advances comprehending an alternative signal transduction pattern, aiming achieve rapid, ultrasensitive, "sample-to-answer" available options progressive applications nanosensors. In summary, cross-collaboration disciplines, has potential break barriers toward commercialization laboratory-level nanosensors, thus leading emergence next generation LFIA.

Language: Английский

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Efficient Photothermal Sensor Based on Coral‐Like Hollow Gold Nanospheres for the Sensitive Detection of Sulfonamides

Small, Journal Year: 2024, Volume and Issue: 20(28)

Published: Feb. 19, 2024

Abstract Gold nanoparticles (AuNPs), universally regarded as colorimetric signal reporters, are widely employed in lateral flow immunoassays (LFIAs). However, it is difficult for AuNPs‐LFIA to achieve a wide range and sensitive detection. Herein, novel coral‐like hollow gold nanospheres (CHGNPs) synthesized. The growth of can be regulated obtain multibranched construction. obtained CHGNPs possess intense broadband absorption across the visible near‐infrared region, exhibiting high molar extinction coefficient 14.65 × 10 11 M −1 cm photothermal conversion efficiency 79.75%. Thus, photothermal/colorimetric dual‐readout LFIA developed based on (CHGNPs‐PT‐LFIA CHGNPs‐CM‐LFIA) effectively improve detection sensitivity broaden regard sulfonamides (SAs). limits CHGNPs‐PT‐LFIA CHGNPs‐CM‐LFIA reached 1.9 2.8 pg mL quantitative sulfaquinoxaline, respectively, which 6.3‐fold 4.3‐fold lower than that AuNPs‐LFIA. Meanwhile, broadened three orders magnitude, ranged from 2.5 5000 . synthesized have been proven effective improving performance provide potential option construction sensing platforms.

Language: Английский

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Photothermal Conversion Porous Organic Polymers: Design, Synthesis, and Applications

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: March 14, 2024

Abstract Solar energy is a primary form of renewable energy, and photothermal conversion direct process with tunable efficiency. Among various kinds materials, porous organic polymers (POP) are widely investigated owing to their controllable molecular design, tailored structures, good absorption solar light, low thermal conductivity. A variety POP, such as conjugated microporous (CMP), covalent frameworks (COF), hyper‐crosslinked (HCP), intrinsic microporosity (PIM), ionic (PIP), developed applied in applications seawater desalination, latent storage, biomedical fields. In this review, comprehensive overview the recent advances POP for provided. The micro structure characteristics macro morphology designed heat phototherapy photodynamic therapy, drug delivery. Besides, probe into underlying mechanism structural design constructing excellent performance methodicalized. Finally, remaining challenges prospective opportunities future development energy‐driven elucidated.

Language: Английский

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Development of a nanobody-based immunoassay for the detection of Escherichia coli O157:H7 in food samples

Food Chemistry, Journal Year: 2025, Volume and Issue: 473, P. 142987 - 142987

Published: Jan. 24, 2025

Language: Английский

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Recent advances in centrifugal microfluidics for point-of-care testing

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.

Language: Английский

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Nanocatalyst-triggered cascade immunoassay: Multi-model immunochromatography assay for sensitive detection of Salmonella typhimurium

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 469, P. 143979 - 143979

Published: June 8, 2023

Language: Английский

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