Sensors,
Journal Year:
2024,
Volume and Issue:
24(19), P. 6458 - 6458
Published: Oct. 6, 2024
Developing
powerful
immunoassays
for
sensitive
and
real-time
detection
of
targets
has
always
been
a
challenging
task.
Due
to
their
advantages
direct
readout,
controllable
sensing,
low
background
interference,
photothermal
have
become
type
new
technology
that
can
be
used
various
applications
such
as
disease
diagnosis,
environmental
monitoring,
food
safety.
By
modification
with
antibodies,
materials
induce
temperature
changes
by
converting
light
energy
into
heat,
thereby
reporting
specific
target
recognition
events.
This
article
reviews
the
design
application
based
on
different
materials,
including
noble
metal
nanomaterials,
carbon-based
two-dimensional
oxide
sulfide
Prussian
blue
nanoparticles,
small
organic
molecules,
polymers,
etc.
It
pays
special
attention
role
working
principle
immunoassays.
Additionally,
challenges
prospects
future
development
are
briefly
discussed.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
Abstract
Immunochromatographic
assays
(ICAs)
provide
simple
and
rapid
strategies
for
bacterial
diagnosis
but
still
suffer
from
the
problems
of
low
sensitivity
high
dependency
on
paired
antibodies.
Herein,
broad‐spectrum
capture
detection
capability
antibody‐free
electropositive
nanoprobe
are
clarified
bacteria
first
time
an
ultrasensitive
fluorescent
ICA
platform
is
constructed
simultaneous
multiple
pathogens.
A
magnetic
multilayer
quantum
dot
nanocomposite
with
amino‐embedded
SiO
2
shell
(MagMQD@Si
+
)
designed
to
enrich
solutions
effectively,
offer
luminescence,
reduce
background
signals
test
strips,
thus
greatly
improving
stability
technique
pathogen.
The
superior
performance
MagMQD@Si
‐based
through
multiplex
three
common
pathogens
demonstrated,
namely,
Pseudomonas
aeruginosa
,
Streptococcus
pneumoniae
typhi
showing
that
this
possesses
(8–40
cells
mL
−1
),
good
reproducibility
(relative
standard
deviation
<
5.4%),
specificity
target
bacteria.
clinical
utility
proposed
method
verified
30
real
sputum
samples
patients
respiratory
infections,
revealing
has
massive
potential
as
a
powerful
inspection
tool
rapid,
sensitive,
infections.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(48), P. 17860 - 17867
Published: Nov. 21, 2023
The
label
with
a
large
Stokes
shift
and
strong
fluorescence
properties
could
improve
the
sensitivity
of
lateral
flow
immunoassay
(LFIA).
Herein,
two
aggregation-induced
emission
(AIE)
luminogens
spectral
overlap
were
encapsulated
in
polymers
by
using
microemulsion
method
as
label,
construction
resonance
energy
transfer
mode
was
further
verified
via
theoretical
calculation
analysis.
Satisfactorily,
doped
AIE
polymer
microspheres
(DAIEPMs)
exhibited
285
nm
10.8-fold
enhancement
compared
to
those
AIEPMs
loaded
acceptors.
Benefiting
from
excellent
optical
performance,
DAIEPMs
applied
LFIA
for
sensitive
detection
chlorothalonil,
which
is
an
organochlorine
pesticide.
limit
proposed
DAIEPMs-LFIA
1.2
pg/mL,
4.8-fold
11.6-fold
lower
than
quantum
dot
bead
gold
nanoparticle
LFIA,
respectively.
This
work
provides
new
strategy
fluorescent
materials
construct
reliable
platform.
Trends in Food Science & Technology,
Journal Year:
2024,
Volume and Issue:
146, P. 104393 - 104393
Published: Feb. 27, 2024
Photothermal
sensing,
due
to
its
high
resolution
and
spatial
controllability,
has
gained
significant
attention
in
optical
imaging
for
applications
such
as
disease
treatment
diagnosis,
environmental
monitoring,
etc.
It
effectively
overcome
the
drawbacks
of
traditional
sensors,
susceptibility
interference,
cost,
lack
portability,
strong
dependency
on
operational
procedures.
conversion-based
light-responsive
temperature
change
sensors
have
been
proven
break
limitations,
serving
an
alternative
strategy
more
potential
applications,
particularly
detection
hazardous
substances
food.
In
current
review,
principles
generation
photothermal
materials
corresponding
representative
are
inducted
modulation
signals
response-dependent
principle
rapid
discussed.
The
practical
food
hazard
outlook
challenges
faced
also
presented
review.
Various
doped
modified
shown
enable
quantitative
hazards
through
different
signal
mechanisms
well
relying
non-specific,
molecular
biological
immunological
responses.
is
hoped
that
review
can
provide
researchers
with
reference
sources
further
developments
effective
sensitive
safety
assurance
industry.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(47), P. 17318 - 17327
Published: Nov. 15, 2023
Although
the
competitive
lateral
flow
immunoassay
(CLFIA)
using
gold
nanoparticles
(AuNPs)
as
labels
has
been
widely
adopted
for
rapid
detection
of
small
molecules,
its
sensitivity
is
often
constrained
by
insufficient
colorimetric
signal
produced
conventional
AuNPs
labels.
Herein,
we
introduce
a
new
type
intensified
label,
denoted
SAAS,
which
engineered
integrating
gold-silver
alloy
(Au-Ag
NPs)
within
dendritic
silica
scaffold.
These
pitaya-type
nanohybrids
combine
advantages
amplified
molar
extinction
coefficient
units
with
collective
effect
numerous
Au-Ag
NPs
in
singular
label.
The
SAAS-based
CLFIA
strips
not
only
achieve
qualitative
screening
aflatoxin
B1
(AFB1)
at
an
extraordinarily
low
concentration
0.2
ng/mL
naked
eye
but
also
enable
precise
AFB1
quantification
through
smartphone,
remarkable
limit
0.00314
ng/mL.
Moreover,
leveraging
SAAS
quencher,
have
delved
into
transforming
signal-off
mode
signal-on
configuration.
This
innovation
led
to
development
fluorescent
LFIA
that
augments
interpretative
precision
and
sensitivity.
Our
study
demonstrates
substantial
potential
proposed
nanohybrid
enhancing
detecting
molecules.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(12), P. 4825 - 4834
Published: Feb. 16, 2024
Immunochromatographic
assays
(ICAs)
have
been
widely
used
in
the
field
detection
of
mycotoxin
contaminants.
Nevertheless,
lack
multisignal
readout
capability
and
ability
signaling
tags
to
maintain
their
biological
activity
while
efficiently
loading
antibodies
remain
a
great
challenge
satisfying
diverse
testing
demands.
Herein,
we
proposed
novel
three-in-one
multifunctional
hollow
vanadium
nanomicrosphere
(high
brightness-catalytic-photothermal
properties)-mediated
triple-readout
ICA
(VHMS-ICA)
for
sensitive
T-2.
As
key
this
biosensing
strategy,
was
as
catalytic-photothermal
characterization
center,
natural
polyphenols
were
utilized
bridging
ligands
coupling
with
antibody
self-assembling
formaldehyde
cross-linking
into
nanocage-like
structure,
which
offers
possibility
realizing
three-signal
strategy
improving
efficiency
preserving
its
activity.
The
constructed
sensors
showed
limit
(LOD)
2
pg/mL
T-2,
about
345-fold
higher
than
that
conventional
gold
nanoparticle-based
(0.596
ng/mL).
anticipated,
range
VHMS-ICA
extended
8-fold
compared
colorimetric
signal
alone.
Ultimately,
immunosensor
performed
well
maize
oat
samples,
satisfactory
recoveries.
Owing
synergistic
complementary
interactions
between
distinct
modes,
establishment
multimodal
immunosensors
is
an
efficient
satisfy
diversified
