Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 26, 2024
The
detection
of
endogenous
phenolic
compounds
(EPs)
in
food
is
great
significance
elucidating
their
bioactivity
and
health
effects.
Here,
a
novel
bifunctional
vanillic
acid-Cu
(VA-Cu)
nanozyme
with
peroxidase-like
laccase-like
activities
was
successfully
prepared.
peroxidase
mimic
behavior
VA-Cu
can
catalyze
3,3',5,5'-tetramethylbenzidine
(TMB)
to
generate
oxidized
TMB
(oxTMB).
Owing
the
high
reducing
power
EPs,
this
process
be
inhibited,
degree
inhibition
increases
increase
reaction
time.
Additionally,
owing
outstanding
laccase
VA-Cu,
it
facilitate
oxidation
various
resulting
formation
colored
quinone
imines,
catalysis
Based
on
interesting
experimental
phenomena
mentioned
above,
six-channel
sensor
array
(2
enzyme-mimic
×
3
time
points
=
6
sensing
channels)
constructed,
achieving
discriminant
analysis
nine
EPs.
In
addition,
combination
artificial
neural
network
(ANN)
algorithms
arrays
has
achieved
accurate
identification
prediction
EPs
black
tea,
honey,
grape
juice.
Finally,
portable
method
for
identifying
been
proposed
by
combining
smartphone.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 16, 2025
Nanozyme-based
colorimetric
sensors
are
promising
approaches
for
environmental
monitoring,
food
safety,
and
medical
diagnostics.
However,
developing
novel
nanozymes
that
exhibit
high
catalytic
activity,
good
dispersion
in
aqueous
solution,
sensitivity,
selectivity,
stability
is
challenging.
In
this
study,
the
first
time,
single-atom
iridium-doped
carbon
dot
(SA
Ir-CDs)
synthesized
via
a
simple
situ
pyrolysis
process.
Doping
dots
with
iridium
form
of
single
atoms
to
achieve
maximum
atomic
utilization
not
only
enhances
peroxidase
(POD)-like
activity
178.81
U
mg-1
but
also
improves
dispersibility
solutions
over
30
days.
Hence,
SA
Ir-CD
platform
developed
mercury
ions
(Hg2+)
detection
exhibited
linear
relationship
from
0.01
10
µm
limit
4.4
nm.
Notably,
changes
color
can
be
observed
through
naked
eye
smartphone,
enabling
convenient
field
onsite
monitoring
without
need
sophisticated
analytical
equipment.
an
approach
fabricating
metal-based
POD-like
developed,
new
effective
easy-to-use
sensor
Hg2+
constructed.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
The
misfolding
of
amyloid
β
(Aβ)
peptides
into
an
aggregation
state
is
a
central
hallmark
the
onset
Alzheimer's
disease
(AD).
However,
conventional
methods
are
mainly
focused
on
detecting
specific
Aβ
peptide,
which
makes
it
difficult
to
recognize
multiple
analytes
with
different
topological
features
and
unfolded
states
at
same
time.
Here,
we
propose
simple
universal
sensing
strategy
construct
fluorescence
sensor
array
by
using
single-nanozyme
probe
combined
three
fluorescent
substrates
as
recognition
units
protein
structural
changes
identify
between
assemblies.
In
this
system,
fingerprint-like
patterns
produced
from
nonspecific
interactions
proteins
units.
As
result,
can
accurately
13
kinds
their
mixtures
ratios.
Moreover,
discriminate
against
diverse
conformational
forms.
Most
importantly,
successfully
distinguishes
species,
even
in
artificial
cerebrospinal
fluid
samples
human
serum
samples.
This
work
provides
attractive
reliable
for
predicting
pathologically
relevant
clinical
diagnosis
AD.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
35(2)
Published: Oct. 4, 2024
Abstract
Single‐atom
nanozyme
materials
have
demonstrated
exceptional
specific
catalytic
activity
due
to
the
atomic‐level
dispersion
of
their
active
centers.
However,
exploration
mechanisms
for
single‐atom
catalysts
is
so
far
limited
2D
surfaces
nanozymes.
In
this
study,
porous
Fe
(psaFeN)
successfully
prepared
through
a
straightforward
coordination‐assisted
polymerization‐assembly
strategy.
The
psaFeN
composite
nanospheres
are
uniformly
sized,
exhibiting
excellent
dispersibility
with
well‐organized
pore
channels
extending
from
center
surface.
Density
functional
theory
calculations
reveal
that
in
nanozyme,
(010)
facets
serve
as
primary
surface,
where
atoms
form
tri‐coordinated
or
tetra‐coordinated
structures
doped
nitrogen
atoms.
(100)
act
auxiliary
reactive
Fe─N
center.
exhibits
POD‐like
(
K
m
=
1.77
mM;
V
max
173.53
×
10
−
⁸
M
s
−1
).
Given
bioactivity,
portable
colorimetric
biosensor
constructed
distinguishing
artificially
ripened
fruits
naturally
ones.
sensor
achieves
precise
discrimination
detection
limit
low
310
nmol
L
.
This
study
anticipated
offer
valuable
insights
into
understanding
3D
nanozymes,
promoting
application
development
robust
biosensors
food
safety.
