Electrochemical Sensors: Types, Applications, and the Novel Impacts of Vibration and Fluid Flow for Microfluidic Integration

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 272, P. 117099 - 117099

Published: Jan. 2, 2025

Language: Английский

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Visual Detection of Canine Monocytic Ehrlichiosis Using Polymerase Chain Reaction-Based Lateral Flow Biosensors

Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 740 - 740

Published: March 5, 2025

A conventional PCR (cPCR) remains an effective molecular technique for the diagnosis of canine monocytic ehrlichiosis. However, agarose gel electrophoresis requires additional time after thermal cycling. In present study, we developed a PCR-based lateral flow biosensor (PCR-LFB) to detect Ehrlichia canis (E. canis). Lateral strips allow simple and rapid detection products provide alternative electrophoresis. The sensitivity, specificity, limit PCR-LFB were compared those TaqMan probe-based real-time PCRs (qPCRs). was performed with 5' 6-FITC biotin-labeled primers specific E. canis, targeting dsb gene. assay 10-6 target DNA sequence in 10-fold dilution recombinant plasmid, which is 10 times lower than that qPCR. Among confirmed qPCR results 30 dog samples, false-positive not detected by PCR-LFB. Compared qPCR, sensitivity specificity 63.6% (95% CI; 42.9-80.2%) 100% 67.5-100%), respectively. Kappa value moderate agreement (κ = 0.483). Perfect 1) observed between cPCR Lower cost shorter consumption demonstrated using

Language: Английский

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Airborne Influenza Virus Surveillance Platform Using Paper-Based Immunosensors and a Growth-Based Virus Aerosol Concentrator

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

The measurement of respiratory viruses in indoor air is critical for effectively preventing the spread diseases. This typically accomplished by counting nucleic acids or plaques air-sampled viruses. Herein, we present a growth-based airborne virus surveillance (G-AVS) platform based on paper-based electrochemical immunosensors targeting hemagglutinin (HA) and nucleoprotein (NP), water-condensation sampling quantitative influenza measurements, compared with RT-qPCR, demonstrated consistency between two. In measurements conducted an elementary school using G-AVS, 23% (4/17) samples were positive, concentrations ranging from 1.7 × 104 to 1.6 106 gene copies/m3, while losses HA relative NP 48–75% at humidity 27.0–36.8% 60 min sampling, similar infectivities reported literature. has potential rapid cost-effective measurement.

Language: Английский

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CLIA immunoassay as an alternative and accurate method to detect Sars-Cov-2 antigen compared to ELISA

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 112(4), P. 116828 - 116828

Published: April 5, 2025

Language: Английский

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Optimizing microfluidic chip for rapid SARS-CoV-2 detection using Taguchi method and artificial neural network PSO

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 23, 2025

Abstract Microfluidic biosensors offer a promising solution for real-time analysis of coronaviruses with minimal sample volumes. This study optimizes biochip the rapid detection SARS-CoV-2 using Taguchi orthogonal table L 9 (3 4 ), which comprises nine groups experiments varying four key parameters: Reynolds number (Re), Damköhler (Da), Schmidt (Sc), and dimensionless position reaction surface (X). Signal-to-noise (S/N) ratios variance (ANOVA) are employed to determine optimal parameters assess their impact on binding kinetics response time device. These obtained correspond Re = 4.10 -2 , Da 1000, Sc 10 5 X 1. Additionally, results highlight as most influential factor, accounting 91%, while has effect 0.3%. Furthermore, an artificial neural network optimization technique, specifically particle swarm (PSO), was utilized predict biosensor performance. Derived from Full 81 ) design experiment, PSO model demonstrates its effectiveness compared conventional multi-layer perception (MLP) model, thus underlining potential in this innovative context.

Language: Английский

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Portable, Quantitative, Real-time Isothermal Nucleic Acid Amplification Test Using Microfluidic Device-Coupled UV-LED Photodiode Detector

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117194 - 117194

Published: Jan. 1, 2025

Language: Английский

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Pico-Scale Digital PCR on a Super-Hydrophilic Microarray Chip for Multi-Target Detection

Micromachines, Journal Year: 2025, Volume and Issue: 16(4), P. 407 - 407

Published: March 30, 2025

The technology of digital polymerase chain reaction (dPCR) is rapidly evolving, yet current devices often suffer from bulkiness and cumbersome sample-loading procedures. Moreover, challenges such as droplet merging partition size limitations impede efficiency. In this study, we present a super-hydrophilic microarray chip specifically designed for dPCR, featuring streamlined loading methods compatible with micro-electro-mechanical systems (MEMS) technology. Utilizing hydrodynamic principles, our platform enables the formation uniform array 120-pL independent units within closed channel. setup allows rapid reactions facilitated by an efficient thermal cycler real-time imaging. We achieved absolute quantitative detection hepatitis B virus (HBV) plasmids at varying concentrations, alongside multiple targets, including cancer mutation gene fragments reference genes. This work highlights chip’s versatility potential applications in point-of-care testing (POCT) diagnostics.

Language: Английский

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Low-cost optical sensors in electrified lab-on-a-disc platforms: liquid-phase boundary detection and automated diagnostics

Microsystems & Nanoengineering, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 7, 2025

Abstract Centrifugal microfluidic platforms are highly regarded for their potential in multiplexing and automation, as well wide range of applications, especially separating blood plasma manipulating two-phase flows. However, the need to use stroboscopes or high-speed cameras monitoring these tasks hinders extensive research commercial settings. In this study, we introduce an innovative cost-effective strategy using array light-dependent resistors (LDRs) optical sensors devices, particularly centrifugal platforms. While LDRs attractive photodetectors, bulky size frequently restricts ability provide high-resolution detection systems. Here, specific waveguides direct light beams from narrow apertures onto surface LDRs. We integrated into electrified Lab-on-a-Disc (eLOD) with wireless connectivity smartphones laptops. This enables many such droplet/particle counting velocity measurement, concentration analysis, fluidic interface multiphase flows, real-time sample volume on platforms, separation alternative costly stroboscope microscopes, imaging. used numerical simulations evaluate various fluids scenarios, which include rotation speeds up 50 rad/s a droplet sizes. For testbed, developed eLOD device analyze red cell (RBC) deformability improve automated sickle anemia by differences RBC during centrifugation sensors’ signals. addition anemia, has facilitate low-cost other medical conditions characterized altered deformability, thalassemia, malaria, diabetes.

Language: Английский

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Acoustic-assisted centrifugal microfluidics for particle/cell separation

Chemical Engineering and Processing - Process Intensification, Journal Year: 2024, Volume and Issue: 201, P. 109803 - 109803

Published: April 29, 2024

Language: Английский

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Fabrication of Cost-Effective Microchip-Based Device Using Sandblasting Technique for Real-Time Multiplex PCR Detection

Micromachines, Journal Year: 2024, Volume and Issue: 15(8), P. 944 - 944

Published: July 24, 2024

The combination of multiplex polymerase chain reaction (mPCR) and microfluidic technologies demonstrates great significance in biomedical applications. However, current microfluidics-based molecular diagnostics face challenges multi-target detection due to their limited fluorescence channels, complicated fabrication process, high cost. In this research, we proposed a cost-effective sandblasting method for manufacturing silicon microchips chip-based microdevice field mPCR detection. atomic force microscopy (AFM) images showed rough surface the sandblasted microchips, leading poor biocompatibility. To relieve inhibitory effect, dip-coated layer bovine serum albumin (BSA) on irregular substrate. optimized coating condition was determined by scanning electron microscope (SEM) energy-dispersive X-ray spectroscopy (EDS) (65 °C 60 min). After sufficient coating, performed on-chip PCR tests with 500 copies/mL Coronavirus Disease 2019 (COVID-19) standard sample within 20 min, microchip displayed higher amplification rate compared dry etching chips. Finally, achieved 50 min screening five resistance genes endophthalmitis pathogens our microdevices, strong specificity reliability. Thus, microchip-based platform not only provides rapid, accessible, effective solution but also enables large-scale microfabrication low-cost convenient way.

Language: Английский

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