Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 116997 - 116997
Published: Nov. 26, 2024
Language: Английский
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: April 16, 2024
Abstract The emerging monkeypox virus (MPXV) has raised global health concern, thereby highlighting the need for rapid, sensitive, and easy-to-use diagnostics. Here, we develop a single-step CRISPR-based diagnostic platform, termed SCOPE (Streamlined CRISPR On Pod Evaluation platform), field-deployable ultrasensitive detection of MPXV in resource-limited settings. viral nucleic acids are rapidly released from rash fluid swab, oral saliva, urine samples 2 min via streamlined lysis protocol, followed by 10-min recombinase polymerase amplification (RPA)-CRISPR/Cas13a reaction. A pod-shaped vest-pocket analysis device achieves whole process reaction execution, signal acquisition, result interpretation. can detect as low 0.5 copies/µL (2.5 copies/reaction) within 15 sample input to answer. We validate developed assay on 102 clinical male patients / volunteers, testing results 100% concordant with real-time PCR. single-molecular level sensitivity minutes simplified procedure performed miniaturized wireless device, which is expected spur substantial progress enable practice application diagnostics techniques point-of-care setting.
Language: Английский
Citations
35
Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117221 - 117221
Published: Jan. 30, 2025
Language: Английский
Citations
1
Small Structures, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 19, 2025
To effectively address crisis emergence of new virus such as monkeypox, a collective and collaborative effort between scientists, engineers, innovators, artists from all ages, regions, diverse fields is required. This review explores holistic approach to addressing the monkeypox by integrating nanobiosensors, artificial intelligence, visual arts, humanities, social sciences. Traditional diagnostic methods are often limited time, accessibility, accuracy, but advancement point‐of‐care smart nanobiosensors offers promising shift toward rapid, precise, accessible diagnostics. They enhance ability screen, diagnose, monitor infections efficiently, contributing better disease management. Beyond technological innovation, essential role sciences in fostering public engagement, understanding, acceptance tools emphasized. Visual arts can illustrate scientific concepts, making them more relatable, while storytelling through various media reduce stigma promote preventive measures. Social provide insights into cultural attitudes, behaviors, health challenges, ensuring that solutions integrated communities. By combining these disciplines, this presents comprehensive framework for resilient global system aligns with One Health principles, emphasizing interconnectedness human, animal, environmental health.
Language: Английский
Citations
1
Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 753 - 753
Published: May 10, 2024
Avian influenza viruses (AIVs) of the H5 subtype rank among most serious pathogens, leading to significant economic losses in global poultry industry and posing risks human health. Therefore, rapid accurate virus detection is crucial for prevention control AIVs. In this study, we established a novel method by utilizing precision CRISPR/Cas12a efficiency RT-RPA technologies. This assay facilitates direct visualization results through blue light lateral flow strips, accurately identifying with high specificity without cross-reactivity against other AIV subtypes, NDV, IBV, IBDV. With thresholds 1.9 copies/μL (blue light) × 103 (lateral strips), our not only competes but also slightly surpasses RT-qPCR, demonstrating an 80.70% positive rate across 81 clinical samples. The RT-RPA/CRISPR-based characterized sensitivity, specificity, independence from specialized equipment. immediate field applicability RT-RPA/CRISPR approach underscores its importance as effective tool early management outbreaks caused
Language: Английский
Citations
7
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 417, P. 136196 - 136196
Published: June 25, 2024
Language: Английский
Citations
6
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: April 3, 2024
Abstract Anaplasma marginale infection is one of the most common tick-borne diseases, causing a substantial loss in beef and dairy production industries. Once infected, pathogen remains cattle for life, allowing parasites to spread healthy animals. Since clinical manifestations anaplasmosis occur late disease, sensitive, accurate, affordable identification crucial preventing controlling infection. To this end, we developed an RPA-CRISPR/Cas12a assay specific A. bovines targeting msp4 gene. Our performed at moderately high temperature, producing fluorescent signals or positive readout lateral flow dipstick, which as sensitive conventional PCR-based DNA amplification. This can detect few 4 copies/μl using marker without cross-reactivity other bovine pathogens. Lyophilized components be stored room temperature extended period, indicating its potential field diagnosis low-resource settings bovines.
Language: Английский
Citations
5
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(3)
Published: May 1, 2024
Abstract MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role gene expression regulation. Salivary miRNA, particular, stands out for its non‐invasive collection method ease of accessibility, offering promising avenues the development point‐of‐care diagnostics a spectrum diseases, including cancer, neurodegenerative disorders, infectious diseases. Such promises rapid precise diagnosis, enabling timely treatment. Despite advancements salivary miRNA‐based testing, challenges persist quantification, multiplexing, sensitivity, specificity, particularly miRNA at low concentrations complex biological mixtures. This work delves into these challenges, focusing on application tests use. We explore biogenesis analyze quantitative disease relevance infection, disorders. also examined recent progress extraction, amplification, multiplexed detection methods. study offers comprehensive view testing (POCT). Its successful advancement could revolutionize early detection, monitoring, management various conditions, enhancing healthcare outcomes. article is categorized under: Diagnostic Tools > Biosensing Nanodevices
Language: Английский
Citations
5
Biosafety and Health, Journal Year: 2024, Volume and Issue: 6(5), P. 260 - 269
Published: Sept. 11, 2024
Language: Английский
Citations
5
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 155995 - 155995
Published: Sept. 1, 2024
Language: Английский
Citations
5
Bioengineering & Translational Medicine, Journal Year: 2025, Volume and Issue: 10(3)
Published: Jan. 2, 2025
Human Mpox disease (MPX) is an endemic zoonotic that develops when patients are infected with the virus (MPXV). MPXV shares a high level of genetic similarity to other poxviruses and clinical presentation MPX similar poxvirus infections which can result in delay diagnosis. In addition, phylogenetically divided into two different clades affects severity disease. recent years, there has been unusual worldwide spread MPXV, leading global public health problem. The most important step fight against rapid, highly specific, accurate Following rapid efforts develop diagnostic tests have gained momentum. Here, MPX, epidemiology, discussed. Furthermore, biochemical tests, molecular their development, point-of-care (PoC) applications reviewed. Molecular technologies such as polymerase chain reaction, recombinase amplification, loop-mediated isothermal amplification methods detect evaluated. Additionally, next-generation combined techniques importance PoC transition explored.
Language: Английский
Citations
0