Multi-targets Detection via Combination of Multi-stimulus-response Engineered Bacteria and Hydrogel-based Separation Platform DOI

Huachao Che,

Xike Tian, Yulun Nie

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 478, P. 135578 - 135578

Published: Aug. 18, 2024

Language: Английский

Principles and challenges of whole cell microbial biosensors in the food industry DOI Open Access

América Selene Gaona Mendoza,

María Fernanda Mendoza Acosta,

Julio Armando Massange Sánchez

et al.

Journal of Food Science, Journal Year: 2024, Volume and Issue: 89(9), P. 5255 - 5269

Published: Aug. 22, 2024

Abstract Whole cell microbial biosensors (WCMB) are mostly genetically modified microorganisms used to detect target molecules as indicators of biological and chemical contaminants well in the identification compounds interest food industry. The specificity sensitivity these achieved through design genetic circuits that make use sequences such promoters, terminators, genes encoding regulatory proteins or reporter proteins, among others. Despite advances WCMBs for their application, significant challenges faced, stability, restrictions, need optimize response times so they can be a competitive detection tool market. This review explores technological progress, potential limitations industry, starting by reviewing operating principles biosensors. importance selecting appropriate chassis cells integration recognition elements transducers maximize effectiveness industry is highlighted.

Language: Английский

Citations

5
Dichloromethyl-Containing Derivatives from Streptomyces agglomeratus 5-2-6, a Soil Bacterium Isolated from the Qinghai–Tibet Plateau DOI
D. Dong, Weidong Zhang, Wei Ding

et al.

Journal of Natural Products, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Six novel dichloromethyl-containing polyketides (1-6), including four pyranone analogs (1-4) and two furanone isomers (5, 6), were isolated from Streptomyces agglomeratus 5-2-6, which was obtained the Qinghai-Tibet Plateau, China. Additionally, new linear dichloromethyl compounds (7, 8) via heterologous expression of agg(A-K) in coelicolor M1152. The structures absolute configurations these primarily elucidated high-resolution ESIMS, NMR, Mo2(OAc)4-induced electronic circular dichroism, application modified Mosher's method. Notably, 1-6 feature a moiety, is reported for first time pyranone- furanone-type natural products. Furthermore, results vivo gene deletion, isotope labeling, vitro enzyme reaction experiments revealed rare single-component flavin-dependent halogenase, AggA, catalyzes dichlorination activated sp3-methylene groups.

Language: Английский

Citations

0
Accelerating the Understanding of Biosensors Through the Lens of Cells: State of the Field, Emerging Directions, Advances, and Challenges DOI
Jasjeet Kaur Narang, Niket Rana, Arjun Chauhan

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Language: Английский

Citations

0
Detection of Short-Chain Chlorinated Aliphatic Hydrocarbons through an Engineered Biosensor with Tailored Ligand Specificity DOI
Dongdong Chen,

Jiadi Zhao,

Shengmin Xu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Short-chain chlorinated aliphatic hydrocarbons (SCAHs), commonly used as industrial reagents and solvents, pose a significant threat to ecosystems human health they infiltrate aquatic environments due extensive usage accidental spills. Whole-cell biosensors have emerged cost-effective, rapid, real-time analytical tools for environmental monitoring remediation. While the broad ligand specificity of transcriptional factors (TFs) often prohibits application such biosensors. Herein, we exploited semirational transition approach in conjunction with positive/negative fluorescence-activated cell sorting (FACS) strategy develop biosensor based on TF AlkS, which is highly specific SCAHs. Furthermore, through promoter-directed evolution, performance was further enhanced. Mutation -10 region constitutive promoter P

Language: Английский

Citations

2
Identification of xenobiotic response element family transcription regulator SadR from sulfonamides-degrading strain Microbacterium sp. HA-8 and construction of biosensor to detect sulfonamides DOI
Qian Li, Mingliang Zhang, Changchang Wang

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 415, P. 131705 - 131705

Published: Oct. 30, 2024

Language: Английский

Citations

2
Multi-targets Detection via Combination of Multi-stimulus-response Engineered Bacteria and Hydrogel-based Separation Platform DOI

Huachao Che,

Xike Tian, Yulun Nie

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 478, P. 135578 - 135578

Published: Aug. 18, 2024

Language: Английский

Citations

1