Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(9), P. 1542 - 1550
Published: Jan. 1, 2024
An electrochemical sensing method is developed based on proximity labeling-assisted multiple signal amplification technique. This enables highly sensitive, selective and stable detection of MUC-1 positive tumor cells.
Language: Английский
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116670 - 116670
Published: Aug. 13, 2024
Language: Английский
Citations
22
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 9, 2025
The accurate diagnosis of pancreatic ductal adenocarcinoma (PDAC) suffers low specify, and sensitivity biomarker detection. In complex biological fluid environments, nonspecific adsorption is prevalent, posing challenges to the detection biomarkers at concentrations. Herein, a highly sensitive selective solution-gated graphene transistor (SGGT) fabricated for miRNA-196a in exosomes diagnose PDAC. antifouling modification on surface gate electrode employed through using bovine serum albumin as common sealing agent poly adenine (polyA8) enhance hydrophilicity. effect background noise effectively reduced. limit reached 1.82 × 10-19 m without need labeling or amplification, time within 25 min. clinical experiments verify that receiver operating characteristic curve values are higher than carbohydrate antigen 19-9 biomarker, high 0.98. can well distinguish PDAC from non-PDAC subjects. SGGT sensor platform demonstrates significant potential milieu samples.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 9, 2025
Early and precise diagnosis of neurodegenerative disorders like Alzheimer's (AD) Parkinson's (PD) is crucial for slowing their progression enhancing patient outcomes. Exosomal microRNAs (miRNAs) are emerging as promising biomarkers due to ability reflect the diseases' pathology, yet low abundance poses significant detection hurdles. This review article delves into burgeoning field electrochemical biosensors, designed exosomal miRNA biomarkers. Electrochemical biosensors offer a compelling solution, combining sensitivity required detect low-abundance with specificity needed discern profiles distinctive neural pathological states. We explore operational principles these including transduction mechanisms that facilitate detection. The also summarizes advancements in nanotechnology, signal enhancement, bioreceptor anchoring, microfluidic integration improve sensor accuracy. evidence use disease analyzed, focusing on clinical impact, diagnostic precision, obstacles faced practical applications. Their potential point-of-care testing regulatory considerations market entry discussed. Looking toward future, highlights forthcoming innovations might revolutionize early processes. impressive sensitivity, specificity, compatibility, track become instrumental diseases, possibly transforming care prognosis.
Language: Английский
Citations
0
Journal of Applied Electrochemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 20, 2025
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137687 - 137687
Published: March 1, 2025
Language: Английский
Citations
0
Biosensors, Journal Year: 2024, Volume and Issue: 14(9), P. 423 - 423
Published: Sept. 2, 2024
The gut microbiome is shaped early in life by dietary and lifestyle factors. Specific compounds the affect growth of different bacterial species production beneficial or harmful byproducts. Dysbiosis has been linked to various diseases resulting from presence bacteria their Existing methods for detecting microbial species, such as microscopic observation molecular biological techniques, are costly, labor-intensive, require skilled personnel. Biosensors, which integrate a recognition element, transducer, amplifier, signal processor, display unit, can convert events into electronic signals. This review provides comprehensive systematic survey scientific publications 2018 June 2024, obtained ScienceDirect, PubMed, Scopus databases. aim was evaluate current state-of-the-art identify knowledge gaps application aptamer biosensors determination microbiota. A total 13 eligible were categorized based on type study: those using bioreceptors (category 1) 2) Point-of-care being developed monitor changes metabolites that may lead disease. They well-suited use healthcare system offer an excellent alternative traditional methods. Aptamers gaining attention due stability, specificity, scalability, reproducibility, low cost, immunogenicity. While there limited research aptamers detect human microbiota, they show promise providing accurate, robust, cost-effective diagnostic monitoring microbiome.
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18262 - 18268
Published: Nov. 1, 2024
Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge the improvement operation conditions required bead-based using typical [Ru(bpy)3]2+/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates imaging and quantitative analysis [Ru(bpy)3]2+-modified immunobeads at low anodic potential. The photovoltage generated by photoanode under light promotes oxidation processes electrode/electrolyte interface, thus considerably lowering onset potential both TPrA ECL emission. anti-Stokes shift between excitation (invisible to human eyes) visible emitted results in clear stable signal from immunobeads. addition, it offers possibility site-selective photoexcitation process. This approach not only meets performance traditional accuracy but also additional benefits lower requirements enhanced stability, providing new perspective optimization commercial immunoassays.
Language: Английский
Citations
2
ACS Materials Letters, Journal Year: 2024, Volume and Issue: unknown, P. 3815 - 3823
Published: July 23, 2024
Language: Английский
Citations
1
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 426, P. 137140 - 137140
Published: Dec. 18, 2024
Language: Английский
Citations
1
Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(9), P. 1542 - 1550
Published: Jan. 1, 2024
An electrochemical sensing method is developed based on proximity labeling-assisted multiple signal amplification technique. This enables highly sensitive, selective and stable detection of MUC-1 positive tumor cells.
Language: Английский
Citations
0