Synaptic cell adhesion molecule Cdh6 identifies a class of sensory neurons with novel functions in colonic motility DOI Creative Commons
Julieta Gomez‐Frittelli, Gabrielle Devienne, Lee Edward Travis

et al.

eLife, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Intrinsic sensory neurons are an essential part of the enteric nervous system (ENS) and play a crucial role in gastrointestinal tract motility digestion. Neuronal subtypes ENS have been distinguished by their electrophysiological properties, morphology, expression characteristic markers, notably neurotransmitters neuropeptides. Here we investigated synaptic cell adhesion molecules as novel type markers ENS. Our work identifies two Type II classic cadherins, Cdh6 Cdh8, specific to mouse colon. We show that Cdh6+ demonstrate all other distinguishing classifications including marker Calcb Nmu , Dogiel morphology AH-type electrophysiology I H current. Optogenetic activation distal colon evokes retrograde colonic motor complexes (CMCs), while pharmacologic blockade rhythmicity-associated current disrupts spontaneous generation CMCs. These findings provide first demonstration selective single neurochemical functional class neurons, critical for

Language: Английский

Interfacing with the Brain: How Nanotechnology Can Contribute DOI Creative Commons
Abdullah Ahmed Ali Ahmed, Núria Alegret, Bethany Almeida

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Interfacing artificial devices with the human brain is central goal of neurotechnology. Yet, our imaginations are often limited by currently available paradigms and technologies. Suggestions for brain-machine interfaces have changed over time, along technology. Mechanical levers cable winches were used to move parts during mechanical age. Sophisticated electronic wiring remote control arisen age, ultimately leading plug-and-play computer interfaces. Nonetheless, brains so complex that these visions, until recently, largely remained unreachable dreams. The general problem, thus far, most technology mechanically and/or electrically engineered, whereas a living, dynamic entity. As result, worlds difficult interface one another. Nanotechnology, which encompasses engineered solid-state objects integrated circuits, excels at small length scales single few hundred nanometers and, thus, matches sizes biomolecules, biomolecular assemblies, cells. Consequently, we envision nanomaterials nanotools as opportunities in alternative ways. Here, review existing literature on use nanotechnology look forward discussing perspectives limitations based authors' expertise across range complementary disciplines─from neuroscience, engineering, physics, chemistry biology medicine, science mathematics, social jurisprudence. We focus but also include information from related fields when useful complementary.

Language: Английский

Citations

2
Direct Stimulation of Gastric Smooth Muscle Cells via Gq Proteins With Light DOI Creative Commons

David Zipf,

Markus Vogt, Udhayabhaskar Sathyanarayanan

et al.

Neurogastroenterology & Motility, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

ABSTRACT Background Optogenetics is a cutting‐edge approach that can enable direct stimulation of gastric smooth muscle cells (SMC) by combining cell‐specific overexpression light‐sensitive proteins with light stimulation. We previously demonstrated optogenetic SMC via depolarization restore contractility and food propulsion could become new treatment strategy for gastroparesis. The human receptor Neuropsin (hOPN5) enables activation G q signaling UV light. Herein, we explore this SMC. Methods used transgenic mouse model expressing hOPN5 in fusion eYFP. Antral longitudinal strips were isometric force measurements whole stomachs intragastric pressure measurements, comparing to other stimuli. Adeno‐associated virus (AAV) serotypes screened efficiency transducing cultured SMC, transduced tested Ca 2+ imaging. Results expression was restricted found ~1/3 the stomach. induced increased only mice similarly electrical field reached approximately 1/3 global muscarinic activation. Importantly, optical remained effective an ex vivo gastroparesis model. AAV 2.5 far most serotype transduction, triggered transients hOPN5. Conclusion tool directly stimulate control Thus, it additional complementary light‐induced membrane motility.

Language: Английский

Citations

0
Wireless, battery-free, remote photoactivation of caged-morphine for photopharmacological pain modulation without side effects DOI
Minsung Kim, Marc López‐Cano, Kaiqing Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 281, P. 117440 - 117440

Published: April 6, 2025

Language: Английский

Citations

0
Synaptic cell adhesion molecule Cdh6 identifies a class of sensory neurons with novel functions in colonic motility DOI Creative Commons
Julieta Gomez‐Frittelli, Gabrielle Devienne, Lee Edward Travis

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 7, 2025

Intrinsic sensory neurons are an essential part of the enteric nervous system (ENS) and play a crucial role in gastrointestinal tract motility digestion. Neuronal subtypes ENS have been distinguished by their electrophysiological properties, morphology, expression characteristic markers, notably neurotransmitters neuropeptides. Here, we investigated synaptic cell adhesion molecules as novel cell-type markers ENS. Our work identifies two type II classic cadherins, Cdh6 Cdh8, specific to mouse colon. We show that Cdh6+ demonstrate all other distinguishing classifications including marker Calcb Nmu , Dogiel morphology AH-type electrophysiology I H current. Optogenetic activation distal colon evokes retrograde colonic motor complexes (CMCs), while pharmacologic blockade rhythmicity-associated current disrupts spontaneous generation CMCs. These findings provide first demonstration selective single neurochemical functional class critical for

Language: Английский

Citations

0
Genetically‐targeted control of the spinal cord and peripheral nervous system DOI Creative Commons
Anakaren Romero-Lozano,

Victoria Koptelova,

Zoya Ahmad

et al.

Journal of intelligent medicine., Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Genetically‐targeted neuromodulation, such as opto‐ or chemogenetics, is important for cell‐type specific neuromodulation different applications. Modulating peripheral nerves a daunting task due to the wide variety of constraints in periphery, size, location, and relative distance other nerves. This has resulted many researchers developing innovative solutions modulate nerves, including types light‐emitting devices optogenetics, ligands designer drugs (DREADDs) use nanotechnology. Although spinal cord part central nervous system, it often site stimulation modulating function periphery anatomy same obstacles modulation review summarizes current efforts genetically‐targeted grouped by applications, going through major areas which advancements have been made. We focus on vivo research rodents but also briefly discuss work nonhuman primates (NHPs). Some clinical translation, long‐term adeno‐associated virus (AAV) safety, are discussed some noninvasive, nonspecific technologies mentioned.

Language: Английский

Citations

0
Synaptic cell adhesion molecule Cdh6 identifies a class of sensory neurons with novel functions in colonic motility DOI Open Access
Julieta Gomez‐Frittelli, Gabrielle Devienne, Lee Edward Travis

et al.

Published: Feb. 21, 2025

Intrinsic sensory neurons are an essential part of the enteric nervous system (ENS) and play a crucial role in gastrointestinal tract motility digestion. Neuronal subtypes ENS have been distinguished by their electrophysiological properties, morphology, expression characteristic markers, notably neurotransmitters neuropeptides. Here we investigated synaptic cell adhesion molecules as novel type markers ENS. Our work identifies two Type II classic cadherins, Cdh6 Cdh8, specific to mouse colon. We show that Cdh6+ demonstrate all other distinguishing classifications including marker Calcb Nmu , Dogiel morphology AH-type electrophysiology I H current. Optogenetic activation distal colon evokes retrograde colonic motor complexes (CMCs), while pharmacologic blockade rhythmicity-associated current disrupts spontaneous generation CMCs. These findings provide first demonstration selective single neurochemical functional class neurons, critical for

Language: Английский

Citations

0
Implantable Bioelectronics for Real-timein vivoRecordings of Enteric Neural Activity DOI Creative Commons
Alexander J. Boys,

Amparo Güemes,

Liang Ma

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 27, 2024

Abstract The gastrointestinal tract is regulated by a complex network of electrically-active cell types that communicate to drive gut function. One these systems, the enteric nervous system, represents primary point contact for host factors influence bodily health and behavior. This division autonomic system unique in both its extensivity, with neurons distributed throughout from esophagus rectum, capability local information processing. However, constant intrinsic extrinsic motion as well sparse distribution constitute has made, access analysis study this important component exceedingly challenging. Here, we present construction validation bioelectronic implant accessing neural distal colon. implant, constructed compatibility novel surgical approach applicable across multiple species, designed be positioned within colonic wall close proximity plexi. We captured multi-frequency electrophysiological responses chemical stimulants showed can record distension activity mimicking motility. also show feasibility utilizing device recording putative single freely-moving rats examining context food intake stress. work marks significant advancement understanding pathways gut-brain axis. Our monitoring demonstrates power real-time areas system. Furthermore, direct communication paves way neuromodulation strategies targeting

Language: Английский

Citations

2
Multifunctional Photonic Nanomaterials and Devices for Digital Photomedicine via Neuro‐Immune Cross‐Talks DOI
Seong‐Jong Kim, Tae‐Yeon Kim,

H. Kim

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Abstract The nervous and immune systems are closely interconnected, influence the onset progress of various diseases. Accordingly, understanding interaction neural system becomes very important for treatment intractable diseases with analysis therapeutic mechanisms, such as autoimmune diseases, neurodegenerative cancers, so on. conventional immunomodulation treatments have been mainly carried out by drug administration, but they suffered from systemic negative side‐effects only limited effects on specific disease. In this Perspective, photonic nanomaterials devices reviewed discussed digitally controlled neurostimulating photomedicine via photobiomodulation optogenetics unique viewpoint neuro‐immune cross‐talks. prospects perspectives to integrate advanced wearable implantable healthcare also provided highlighted revolutionize strategies systems, optimize protocols futuristic digital photomedicine. This approach will fields neurostimulation regulation further clinical applications.

Language: Английский

Citations

2
Synaptic cell adhesion moleculeCdh6identifies a class of sensory neurons with novel functions in colonic motility DOI Creative Commons
Julieta Gomez‐Frittelli, Gabrielle Devienne, Lee Edward Travis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Intrinsic sensory neurons are an essential part of the enteric nervous system (ENS) and play a crucial role in gastrointestinal tract motility digestion. Neuronal subtypes ENS have been distinguished by their electrophysiological properties, morphology, expression characteristic markers, notably neurotransmitters neuropeptides. Here we investigated synaptic cell adhesion molecules as novel type markers ENS. Our work identifies two Type II classic cadherins,

Language: Английский

Citations

0
Wirelessly-Powered Ingestible Electronic Capsule for Non-invasive Gastrointestinal Optogenetics DOI Open Access
Mohamed Elsherif,

Rawan Badr El-Din,

Zhansaya Makhambetova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Abstract Optogenetics enables the activation and inhibition of neurons with cell specificity. The gut harbors intricate networks enteric central neurons. Uncovering these neuronal pathways in vivo is challenging traditional neuroscience probes due to highly motile harsh environment. Here we report development an ingestible electronic capsule for non-invasive optical stimulation (ICOPS) rodents. ICOPS powered wirelessly via a transmitter coil, dosed oral gavage, safely excreted without causing obstruction. permits modular interchangeability onboard light-emitting diodes (LEDs) illumination. We exemplify this irradiance at 470 nm, commonly-used wavelength optogenetics activating channelrhodopsin2. features micro-LED (µLED), 460-turn coil wound around ferrite core, resonating capacitor. optimized transmitting receiving circuits achieve maximum power transfer low operating frequencies (45-140 kHz), overcoming challenges like loose coupling misalignment. operates effectively distance up 12 cm longitudinally, 9 laterally, 75° rotational angle relative magnetic field. Specific absorption rate (SAR) calculations indicate transmitter-induced SAR levels within safe limits occupational environment 6 A rms 45 63 kHz robust transits through rat gastrointestinal (GI) tract under 20 hours intact. demonstrate functionality viability using IVIS micro-computed tomography (µCT). could pave way optogenetic interfacing neural towards their use regulate motility, visceral pain, other disorders.

Language: Английский

Citations

0