Oxygen Activation Biocatalytic Precipitation Strategy Based on a Bimetallic Single-Atom Catalyst for Photoelectrochemical Biosensing

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

The traditional biocatalytic precipitation (BCP) strategy often required the participation of H2O2, but H2O2 had problem self-decomposition, which prevented its application in quantitative analysis. This work first found that a bimetallic single-atom catalyst (Co/Zn–N–C SAC) could effectively activate dissolved O2 to produce reactive oxygen species (ROS) due superior oxidase (OXD)-like activity. Experimental investigations demonstrated Co/Zn–N–C SAC preferred highly active hydroxyl radicals (•OH), oxidized 3-amino-9-ethyl carbazole (AEC) reddish-brown insoluble precipitates. Based on this property, unique oxygen-activated photoelectrochemical (PEC) biosensor was developed for chloramphenicol (CAP) detection. Cesium platinum bromide nanocrystals (Cs2PtBr6 NCs) were new type halide perovskite with lead-free, narrow band gaps, and water-oxygen resistance. Cs2PtBr6 NCs showed excellent cathodal PEC performance without an exogenous coreactant used As "proof-of-concept application", introduced onto surface by using CAP dual-aptamer sandwich strategy. activated ROS, AEC precipitates, quenching signal In summary, overcome restriction enzymatic BCP requiring improved stability accuracy analysis, also broadened range coreactant-free perovskite-type biosensors.

Language: Английский

Novel Dual-Potential Color-Resolved Luminophore Ru(bpy)₃²⁺-Doped CdSe QDs for Bipolar Electrode Electrochemiluminescence Biosensing

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

The classical electrochemiluminescence (ECL) reagent Ru(bpy)32+ was first doped into CdSe QDs to prepare novel dual-potential color-resolved luminophore Ru-CdSe QDs. emitted a strong red ECL signal at positive potential with coreactant TPrA and green negative K2S2O8. As proof-of-concept application, this work introduced dual-channel closed bipolar electrode (CBPE) system construct an biosensor for simultaneous detection of chloramphenicol (CAP) kanamycin (KAN). were dropped on BPE holes A B emission. Cobalt single-atom catalysts (Co–N–C SACs) had superior electric double layer (EDL) performance conductivity. It could greatly promote the electron transfer CBPE realize amplification. Based characteristic, Co–N–C SACs hole C driving D, respectively, using CAP KAN split dual-aptamer sandwich strategy. During scanning, polarity anode, signal. With increase concentration, abundant surface. increased detection. cathode, Finally, zero-background spatial-potential CBPE–ECL developed dual-mode KAN. This explored sensor, which facilitated development assay.

Language: Английский