From Bench to Bedside: The Role of Extracellular Vesicles in Cartilage Injury Treatment DOI Creative Commons
Pan Jin, Huan Liu,

Xichi Chen

et al.

Biomaterials Research, Journal Year: 2024, Volume and Issue: 28

Published: Jan. 1, 2024

Cartilage repair is the key to treatment of joint-related injury. However, because cartilage lacks vessels and nerves, its self-repair ability extremely low. Extracellular vesicles (EVs) are bilayer nanovesicles with membranes mainly composed ceramides, cholesterol, phosphoglycerides, long-chain free fatty acids, containing DNA, RNA, proteins (such as integrins enzymes). For mediating intercellular communication regulating mechanisms, EVs have been shown by multiple studies be effective options for repair. This review summarizes recent findings different sources (mammals, plants, bacteria) uses in repair, mechanisms captured injured chondrocytes, quantification storage EVs, which may provide scientific guidance promoting development field injury treatment.

Language: Английский

CD63 as a potential biomarker for patients with ovarian cancer DOI
Keiichiro Nakamura,

Naoyuki Ida,

Akira Hirasawa

et al.

European Journal of Obstetrics & Gynecology and Reproductive Biology, Journal Year: 2025, Volume and Issue: 306, P. 87 - 93

Published: Jan. 7, 2025

Language: Английский

Citations

0
Single-cell immunoassay of breast cancers based on their exosomal receptor expression profiles DOI
Xin Wei Wang, Jiaqi Wang, Shuping Xu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159904 - 159904

Published: Jan. 1, 2025

Language: Английский

Citations

0
Isolation and detection strategies for decoding the heterogeneity of extracellular vesicles DOI
Shiming Wang, Dan Wang, Yuqing Shen

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160234 - 160234

Published: Feb. 1, 2025

Language: Английский

Citations

0
Engineered extracellular vesicles: an emerging nanomedicine therapeutic platform DOI

Jingshi Tang,

Dezhong Li, Rui Wang

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Extracellular vesicles have been deemed as potential drug carriers for treatment of various diseases. Recent advances summarized, including the sources, delivery function, extraction and cargo-loading technology extracellular vesicles.

Language: Английский

Citations

0
Enhancing Analysis of Extracellular Vesicles by Microfluidics DOI
Jiayu Sun,

Zongbo Li,

Yonghui Chen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Extracellular vesicles (EVs) play crucial roles in intercellular communication and hold great promise as biomarkers for noninvasive disease diagnosis. Intensive research efforts have been devoted to discovering the EV subpopulations responsible specific functions or with enhanced effectiveness markers, through extensive purification content analysis. However, their high heterogeneity size cargo composition poses significant challenges reaching such goals. Isolation methods like ultracentrifugation size-exclusion chromatography, well analysis approaches polymerase chain reaction enzyme-linked immunosorbent assay, made contributions improving our understanding of biology. Nonetheless, these face limitations isolation efficiency, purity, detection sensitivity specificity due issues large sample consumption, unsatisfactory insufficient resolution subtyping. Microfluidic technology presents promising solutions challenges, leveraging intrinsic capabilities precise flow external energy field manipulation, compartmentalization, signal enhancement at micro- nanoscale. Hence, this review summarizes recent developments microfluidics-enabled analysis, paying special attention unique microfluidic features exploited. Strategies viscoelastic inertial flow, fluid mixing, external-field-assisted purification, compartmentalization micro/nanostructures enhancing detection, are examined. Furthermore, current potential future directions discussed inspire advancements rapidly developing field.

Language: Английский

Citations

0
Functional Molecule‐Integrated Nano‐Interfaces for Detection of Extracellular Vesicles DOI Creative Commons
Hirobumi Sunayama,

Yuya Matsui,

Toshifumi Takeuchi

et al.

Advanced Sensor Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Functional molecule‐integrated polymer nanocavities for the detection of extracellular vesicles (EVs) are prepared via template polymerization and multistep post‐modification. A multifunctional bearing a tertiary amino group interaction with silica nanoparticles (NPs) polymerizable methacryl is complexed 500‐nm NPs. The complex monodispersedly immobilized on carboxyl Br introduced onto Au‐coated glass substrate. Additionally, surface‐initiated atom transfer radical 2‐methacryloyloxyethyl phosphorylcholine conducted to form biocompatible layer. After NPs removed, disulfide bond cleaved using tris(2‐carboxyethyl)phosphine remove components, yielding thiol group‐exposed nanocavity. degree functionalization controlled by changing mixing ratio fluorescent non‐fluorescent dyes. multifunctionalized nanocavity introducing dye nitrilotriacetic acid moiety, followed His‐tagged protein G an antibody CD9 protein, EV‐sensing nano‐interfaces. This signaling interface responds EVs in concentration‐dependent manner, estimated limit 0.1 fM. These responses not observed when reference introduced, confirming cavity‐selective functionalization. proposed method useful fabricating nano‐interfaces field life sciences.

Language: Английский

Citations

0
A Fully Enclosed Sliding RNA Detection Chip and device for Point-of-Care Testing DOI
Biao Li,

Fenlin Chen,

Xinhua Su

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137884 - 137884

Published: May 1, 2025

Language: Английский

Citations

0
Rapid Detection of Drugs in Blood Using "Molecular Hook" Surface-Enhanced Raman Spectroscopy and Artificial Intelligence Technology for Clinical Applications DOI

Qingbin Wei,

Liping Zhou, Jin Sun

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 267, P. 116855 - 116855

Published: Oct. 16, 2024

Language: Английский

Citations

3
From Bench to Bedside: The Role of Extracellular Vesicles in Cartilage Injury Treatment DOI Creative Commons
Pan Jin, Huan Liu,

Xichi Chen

et al.

Biomaterials Research, Journal Year: 2024, Volume and Issue: 28

Published: Jan. 1, 2024

Cartilage repair is the key to treatment of joint-related injury. However, because cartilage lacks vessels and nerves, its self-repair ability extremely low. Extracellular vesicles (EVs) are bilayer nanovesicles with membranes mainly composed ceramides, cholesterol, phosphoglycerides, long-chain free fatty acids, containing DNA, RNA, proteins (such as integrins enzymes). For mediating intercellular communication regulating mechanisms, EVs have been shown by multiple studies be effective options for repair. This review summarizes recent findings different sources (mammals, plants, bacteria) uses in repair, mechanisms captured injured chondrocytes, quantification storage EVs, which may provide scientific guidance promoting development field injury treatment.

Language: Английский

Citations

2