N-Doped Porous Carbon Synergistic Freezing-Induced DNA with Catalyzed Hairpin Assembly Enables Electrochemical One-Pot Detection of Pathogen in Food Samples

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

A DNA electrochemical interface biosensor based on screen-printed carbon electrodes (SPCEs) holds promise for point-of-care testing (POCT) detection of pathogens in food safety. Nevertheless, SPCE commonly has a rough surface and suffers from relatively low electron transfer rate, disorder capture probes (CPs), the steric hindrance effect target nucleic acid binding. These issues lead to sensitivity. Herein, simple rapid N-doped porous (NPC)-modified freezing-directed combined with catalyzed hairpin assembly (CHA) was constructed one-pot samples without time-consuming growth cultures. The by modified NPC enhanced properties, CP designed CHA stably fixed electrode high hybridization efficiency. Moreover, signals amplified enable selective sensitive washing steps. This method is wide linear range 101 107 CFU/mL limit 5 Escherichia coli shows specificity against other coexisting pathogens. whole complex performed only within 60 min sample-to-answer, which great potential POCT

Language: Английский

Aptamer-based dual-enzyme, amplification-free biosensor integrating CRISPR-Cas12a and Exo III for sensitive detection of ATP

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100795 - 100795

Published: April 1, 2025

Language: Английский