Biotechnology for Biofuels,
Journal Year:
2021,
Volume and Issue:
14(1)
Published: March 8, 2021
Abstract
Background
The
filamentous
fungus
Trichoderma
reesei
is
one
of
the
best
producers
cellulase
and
has
been
widely
studied
for
production
cellulosic
ethanol
bio-based
products.
We
previously
reported
that
Mn
2+
N
,
-dimethylformamide
(DMF)
can
stimulate
overexpression
via
Ca
bursts
calcium
signalling
in
T.
under
cellulase-inducing
conditions.
To
further
understand
regulatory
networks
involved
we
characterised
/DMF-induced
pathway
stimulation
overexpression.
Results
found
/DMF
significantly
increased
intracellular
levels
cAMP
an
adenylate
cyclase
(ACY1)-dependent
manner.
Deletion
acy1
confirmed
crucial
/DMF-stimulated
.
revealed
elevation
induces
a
cytosolic
burst,
thereby
initiating
signal
transduction
causes
to
regulate
Furthermore,
using
phospholipase
C
encoding
gene
plc-e
deletion
strain,
showed
vital
response
by
both
DMF,
burst
through
PLC-E.
Conclusions
findings
this
study
reveal
presence
which
produces
cAMP.
Increase
activates
These
provide
insights
into
molecular
mechanism
cAMP–PLC–calcium
underlying
expression
highlight
potential
applications
regulation
fungi.
Biotechnology for Biofuels,
Journal Year:
2020,
Volume and Issue:
13(1)
Published: Feb. 1, 2020
Abstract
Xylose
is
a
major
component
of
lignocellulose
and
the
second
most
abundant
sugar
present
in
nature.
Efficient
utilization
xylose
required
for
development
economically
viable
processes
to
produce
biofuels
chemicals
from
biomass.
However,
there
are
still
some
bottlenecks
bioconversion
xylose,
including
fact
that
microorganisms
cannot
assimilate
naturally
uptake
metabolism
inhibited
by
glucose,
which
usually
with
hydrolysate.
To
overcome
these
issues,
numerous
efforts
have
been
made
discover,
characterize,
engineer
transporters
enzymes
involved
relieve
glucose
inhibition
develop
recombinant
fuels
xylose.
Here
we
describe
recent
advancement
focusing
on
xylose-utilizing
pathways,
biosynthesis
engineering
strategies
used
improve
conversion
efficiency
Discover Energy,
Journal Year:
2024,
Volume and Issue:
4(1)
Published: June 14, 2024
Abstract
This
comprehensive
review
aims
to
explore
and
elucidate
the
pivotal
role
of
biotechnology
in
biofuel
production,
specifically
focusing
on
its
contribution
enhancing
sustainability,
efficiency,
productivity
energy
sector.
By
examining
various
biotechnological
approaches
like
genetic
engineering,
metabolic
synthetic
biology,
it
seeks
provide
insights
into
effectively
harnessing
generation
processes,
including
integration
machine
learning
life
cycle
assessment
for
microalgae
cultivation
harvesting.
Additionally,
sheds
light
multifaceted
implications
surrounding
production
consumption,
addressing
technological,
ethical,
social,
economic
considerations.
Through
critical
analysis
advantages
challenges
associated
with
biotechnology-driven
development,
offers
a
balanced
perspective
true
potential
biofuels
as
viable,
sustainable,
equitable
source
future.
examination
provides
holistic
symbiotic
relationship
between
biofuels,
highlighting
how
advancements
techniques
can
pave
way
more
sustainable
resilient
both
promises
limitations
this
context,
contribute
informed
decision-making
policy
formulation
drive
transition
toward
cleaner
paradigm.
Graphical
Biotechnology for Biofuels,
Journal Year:
2020,
Volume and Issue:
13(1)
Published: April 1, 2020
The
filamentous
fungus
Trichoderma
reesei
is
a
major
workhorse
employed
to
produce
cellulase,
which
hydrolyzes
lignocellulosic
biomass
for
the
production
of
cellulosic
ethanol
and
bio-based
products.
However,
economic
efficiency
biorefineries
still
low.In
this
study,
truncation
cellulase
activator
ACE3
was
identified
characterized
in
T.
classical
mutant
NG14
its
direct
descendants
first
time.
We
demonstrated
that
truncated
crucial
cause
hyper-production
branch.
Replacing
native
with
other
strains
remarkably
improves
production.
By
truncating
ACE3,
we
engineered
mutant,
PC-3-7-A723,
capable
producing
more
than
strains.
In
30-L
fermenter,
fed-batch
fermentation
PC-3-7-A723
drastically
increased
maximum
titer
(FPase)
102.63
IU/mL
at
240
h,
constitutes
20-30%
improvement
parental
strain
PC-3-7.This
work
function
analysis
mutants
allows
rational
engineering
improved
necessary
process
biomass.
Our
strategy
might
be
useful
enhancing
