Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application

Frontiers in Bioengineering and Biotechnology, Journal Year: 2020, Volume and Issue: 8

Published: Sept. 3, 2020

Microalgae, due to their complex metabolic capacity, are being continuously explored for nutraceuticals, pharmaceuticals and other industrially important bioactives. However, suboptimal yield productivity of the bioactive interest in local robust wild-type strains perennial concerns industrial applications. To overcome such limitations, strain improvement through genetic engineering could play a decisive role. Though advanced tools have emerged at greater pace, they still remains under-used microalgae as compared microorganisms. Pertaining this, we reviewed progress made so far development molecular techniques, deployment engineering. The recent availability genome sequences omics datasets form diverse species remarkable potential guide strategic momentum program. This review focuses on significant improvements resources, mutant libraries high throughput screening methodologies helpful augment research model non-model microalgae. Authors also summarized case studies genetically engineered microalgae, highlight opportunities challenges that emerging from current application genome-editing facilitate microalgal improvement. Towards end, regulatory biosafety issues use commercial applications described.

Language: Английский

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Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: A review

Progress in Energy and Combustion Science, Journal Year: 2023, Volume and Issue: 96, P. 101071 - 101071

Published: Feb. 27, 2023

Microalgae have gained considerable attention as an alternative feedstock for the biofuel production, particularly in combination with genetic modification strategies that target enhanced lipid productivity. To tackle climate change issues, phasing out usage of fossil fuels is seen a priority, where utilization from microalgae serves potential sustainable energy source various applications. These photosynthetic utilize solar and carbon dioxide to produce energy-rich compounds (i.e., starch lipids), can be further converted into biofuels different types. Among types biofuels, biodiesel transesterification triacylglycerols stands most replacement transportation fuel over fossil-based petroleum diesel. However, hurdles such limited productivity, overall production cost challenges upscaling algal technology leaves huge gap on road commercialized microalgae-based biofuel. This review article first presents comprehensive overview imperative knowledge regarding terms classification, factors affecting growth during cultivation steps upstream processing. also discusses recent advances downstream processing microalgal biorefinery. Additionally, this paper focuses deliberating modifications their feasibility productivity microalgae. Finally, current future perspectives are highlighted discussing several aspects, including sustainability status algae-based industry, risks legislation considerations

Language: Английский

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Two-stage cultivation of microalgae for production of high-value compounds and biofuels: A review

Algal Research, Journal Year: 2021, Volume and Issue: 57, P. 102353 - 102353

Published: May 27, 2021

Language: Английский

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Enzymatic Conversion of CO₂: From Natural to Artificial Utilization

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(9), P. 5702 - 5754

Published: Jan. 24, 2023

Enzymatic carbon dioxide fixation is one of the most important metabolic reactions as it allows capture inorganic from atmosphere and its conversion into organic biomass. However, due to often unfavorable thermodynamics difficulties associated with utilization CO

Language: Английский

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Using green alga Haematococcus pluvialis for astaxanthin and lipid co-production: Advances and outlook

Bioresource Technology, Journal Year: 2021, Volume and Issue: 340, P. 125736 - 125736

Published: Aug. 10, 2021

Language: Английский

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Current trends in biodiesel production technologies and future progressions: A possible displacement of the petro-diesel

Journal of Cleaner Production, Journal Year: 2022, Volume and Issue: 370, P. 133479 - 133479

Published: Aug. 12, 2022

Language: Английский

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Large-scale production of Spirulina-based proteins and c-phycocyanin: A biorefinery approach

Biochemical Engineering Journal, Journal Year: 2022, Volume and Issue: 185, P. 108541 - 108541

Published: July 1, 2022

Language: Английский

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Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(7), P. 440 - 440

Published: June 30, 2022

Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand food feed. However, microalgae-based products are currently hindered by high production costs. One major reason this is that commonly cultivated wildtype strains do not possess robustness productivity required successful industrial production. Several strain improvement technologies been developed towards creating more stress tolerant productive strains. While classical methods of forward genetics extensively used to determine gene function randomly generated mutants, reverse has explored generate specific mutations target phenotypes. Site-directed mutagenesis can be accomplished employing different editing tools, which enable generation tailor-made genotypes. Nevertheless, strategies promoting selection mutants avoid introduction foreign genetic material. In paper, we review microalgal approaches their applications, with primary focus on random mutagenesis. Current challenges hampering improvement, selection, commercialization will discussed. The combination these high-throughput technologies, such as fluorescence-activated cell sorting, tools select most also

Language: Английский

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Advancements on process regulation for microalgae-based carbon neutrality and biodiesel production

Renewable and Sustainable Energy Reviews, Journal Year: 2022, Volume and Issue: 171, P. 112969 - 112969

Published: Oct. 25, 2022

Language: Английский

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Microalgae biorefinery: An integrated route for the sustainable production of high-value-added products

Energy Conversion and Management X, Journal Year: 2022, Volume and Issue: 16, P. 100323 - 100323

Published: Nov. 2, 2022

Microalgae are pervasive organisms regarded as conservative alternatives for the sustainable synthesis of various high-value-added bio-products to replace depleting fossil fuels and reduce CO2 emissions. However, microalgae biorefinery is still very far from being commercially viable due high costs, intensive energy requirements, low availability biomass biomolecules. In this review, current status discussed by establishing efficiency green biotechnology in producing wastewater diverse derived biorefinery. addition, aspects involved production bulk chemicals value-added biorefinery, including their roles environmental protection promotion an integrated circular bio-economy elucidated. Lastly, challenges facing recommendations guide future research toward cost-effective, more efficient, presented.

Language: Английский

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