Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 5, 2025
Abstract
Manipulation
of
single
cells
or
particles
is
crucial
in
the
biomedical
field.
However,
precisely
and
rapidly
manipulating
without
damaging
them
a
significant
challenge.
In
this
study,
novel
strategy
for
indirect
manipulation
microparticles
that
can
satisfy
these
requirements
via
combination
particle‐induced
dielectrophoretic
forces
(PiDEP)
optoelectronic
tweezers
(OET)
developed.
This
based
primarily
on
principle
experiencing
same
tend
to
repel
each
other,
whereas
those
different
are
attracted
other.
During
manipulation,
Ag‐SiO
2
controlled
by
OET
act
as
intermediaries
other
through
forces.
Thus,
range
be
expanded
two
three
times
its
original
size,
speed
significantly
increased
while
maintaining
precision.
Furthermore,
results
indicate
proposed
method
effectively
reduce
cell
damage
one‐third
caused
traditional
OET.
study
demonstrates
potential
particle‐assisted
single‐cell
offers
an
effective
microparticles.
Annual Review of Biophysics,
Journal Year:
2024,
Volume and Issue:
53(1), P. 367 - 395
Published: Feb. 21, 2024
The
mechanical
phenotype
of
a
cell
determines
its
ability
to
deform
under
force
and
is
therefore
relevant
cellular
functions
that
require
changes
in
shape,
such
as
migration
or
circulation
through
the
microvasculature.
On
practical
level,
can
be
used
global
readout
cell's
functional
state,
marker
for
disease
diagnostics,
an
input
tissue
modeling.
We
focus
our
review
on
current
knowledge
structural
components
contribute
determination
properties
highlight
physiological
processes
which
cells
critical
relevance.
ongoing
efforts
understand
how
efficiently
measure
control
will
define
progress
field
drive
phenotyping
toward
clinical
applications.
npj Biological Physics and Mechanics.,
Journal Year:
2025,
Volume and Issue:
2(1)
Published: Feb. 4, 2025
Abstract
The
cancer
metastatic
cascade
includes
a
series
of
mechanical
barrier-crossing
events,
involving
the
physical
movement
cells
from
their
primary
location
to
distant
organ.
This
review
describes
changes
that
influence
tumour
proliferation,
progression,
and
metastasis.
We
identify
potential
signatures
at
every
step
discuss
some
latest
mechanobiology-based
therapeutic
interventions
highlight
importance
interdisciplinary
approaches
in
diagnosis
treatment.
Biomarker Research,
Journal Year:
2025,
Volume and Issue:
13(1)
Published: Jan. 23, 2025
The
tumor
microenvironment
functions
as
a
dynamic
and
intricate
ecosystem,
comprising
diverse
array
of
cellular
non-cellular
components
that
precisely
orchestrate
pivotal
behaviors,
including
invasion,
metastasis,
drug
resistance.
While
unraveling
the
interplay
between
behaviors
represents
tremendous
challenge,
recent
research
illuminates
crucial
biological
phenomenon
known
mechanotransduction.
Within
microenvironment,
mechanical
cues
like
tensile
stress,
shear
stiffness
play
role
by
activating
mechanosensitive
effectors
such
PIEZO
proteins,
integrins,
Yes-associated
protein.
This
activation
initiates
cascades
intrinsic
signaling
pathways,
effectively
linking
physical
properties
tissues
to
their
physiological
pathophysiological
processes
morphogenesis,
regeneration,
immunity.
mechanistic
insight
offers
novel
perspective
on
how
within
impact
behaviors.
intricacies
are
yet
be
fully
elucidated,
it
exhibits
distinct
attributes
from
non-malignant
tissues,
elevated
solid
stresses,
interstitial
hypertension,
augmented
matrix
stiffness,
enhanced
viscoelasticity.
These
traits
exert
notable
influences
progression
treatment
responses,
enriching
our
comprehension
multifaceted
nature
microenvironment.
Through
this
innovative
review,
we
aim
provide
new
lens
decipher
contexts,
broadening
knowledge
these
factors
promote
or
inhibit
thus
offering
valuable
insights
identify
potential
targets
for
anti-tumor
strategies.
Lab on a Chip,
Journal Year:
2022,
Volume and Issue:
22(8), P. 1438 - 1468
Published: Jan. 1, 2022
Single-cell
cultivation
is
essential
to
investigate
the
actual
cellular
and
subcellular
information
of
individual
cells
enhance
single-cell
colonies
cell-derived
products
for
further
analysis
at
a
level.
Analytical Chemistry,
Journal Year:
2020,
Volume and Issue:
93(3), P. 1586 - 1595
Published: Dec. 8, 2020
Yeast
Saccharomyces
cerevisiae
(S.
Cerevisiae)
is
one
of
the
most
attractive
microbial
species
used
for
industrial
production
value-added
products
and
an
important
model
organism
to
understand
biology
eukaryotic
cells
humans.
S.
Cerevisiae
has
different
shapes,
such
as
spherical
singlets,
budded
doublets,
clusters,
corresponding
phases
cell
cycle,
genetic,
environmental
factors.
The
ability
obtain
high-purity
populations
uniform-shaped
significant
importance
a
wide
range
applications
in
basic
biological
research
processes.
In
this
work,
we
demonstrate
shape-based
separation
enrichment
using
coflow
viscoelastic
Newtonian
fluids
straight
rectangular
microchannel.
Due
combined
effects
lift
inertial
elastic
forces,
label-free
continuous
arises
from
shape-dependent
migration
non-Newtonian
fluid.
lateral
position
with
varying
morphologies
found
be
dependent
on
major
axis.
We
also
investigate
sheath
sample
flow
rate,
poly(ethylene
oxide)
(PEO)
concentration
channel
length
performance
microfluidic
device
by
shape.
Moreover,
efficiency,
extraction
yield,
viability
after
sorting
operations
are
studied.
Frontiers in Physics,
Journal Year:
2021,
Volume and Issue:
9
Published: June 21, 2021
Changes
in
biomechanical
properties
of
biological
soft
tissues
are
often
associated
with
physiological
dysfunctions.
Since
hydrated,
viscoelasticity
is
likely
suitable
to
represent
its
solid-like
behavior
using
elasticity
and
fluid-like
viscosity.
Shear
wave
elastography
a
non-invasive
imaging
technology
invented
for
clinical
applications
that
has
shown
promise
characterize
various
tissue
viscoelasticity.
It
based
on
measuring
analyzing
velocities
attenuations
propagated
shear
waves.
In
this
review,
principles
technical
developments
characterization
from
organ
cellular
levels
presented,
different
modalities
used
track
propagation
described.
At
macroscopic
scale,
techniques
inducing
waves
an
external
mechanical
vibration,
acoustic
radiation
pressure
or
Lorentz
force
reviewed
along
approaches
proposed
propagation,
namely
ultrasound,
magnetic
resonance,
optical,
photoacoustic
means.
Then,
theoretical
modeling
tracking
detailed.
Following
it,
some
examples
the
organs
given.
microscopic
novel
method
vibration
optical
microscopy
illustrated.
Finally,
current
limitations
future
directions
presented.
IEEE Transactions on Biomedical Engineering,
Journal Year:
2022,
Volume and Issue:
70(2), P. 565 - 572
Published: Aug. 8, 2022
Deformability
is
an
essential
feature
of
red
blood
cells
(RBCs),
enabling
them
to
undergo
significant
shape
change
in
response
external
forces.
Impaired
erythrocyte
deformability
associated
with
several
pathologic
conditions,
and
quantitative
measurement
RBC
critical
understanding
diagnosing
related
diseases.
Whereas
traditional
approaches
cell
mechanical
characterization
generally
have
limited
throughput,
emerging
microscale
technologies
are
opening
new
opportunities
for
high-throughput
cytometry
at
the
single-cell
level.In
this
work,
we
propose
innovative
microfluidic
system
based
on
(i)
a
hyperbolic
microchannel
induce
deformation
by
extensional
flow,
(ii)
electrical
sensing
zone
coplanar
electrodes
evaluate
deformed
shape.RBC
under
flow
achieved,
quantified
means
anisotropy
index,
throughput
300
cell/s.
Measurements
healthy
chemically
stiffened
RBCs
demonstrate
that
index
can
be
used
characterize
deformability,
as
alternative
indices
high-speed
image
processing.A
contactless
optics-free
approach
analysis
has
been
presented.Due
its
simplicity
potential
integration,
proposed
holds
promises
fast
low-cost
assays,
especially
point-of-care
resource-limited
settings.
