Zeitschrift für Naturforschung C,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 13, 2023
Wood
decomposing
ascomycetes
and
basidiomycetes
group
of
fungi
are
the
most
valuable
microbes
on
earth's
ecosystem
that
recycles
source
carbon;
therefore,
they
essential
for
biorefinery
industries.
To
understand
robustness
enzymes
their
metabolic
pathways
in
fungal
system,
label-free
quantification
total
proteins
was
performed.
The
showed
a
comparable
quantity
protein
abundance
[Trichoderma
citrinoviride
(285),
Thermoascus
aurantiacus
(206),
Ganoderma
lucidum
MDU-7
(102),
G.
(242)].
Differentially
regulated
were
analyzed,
heatmap
shows
upregulated
downregulated
[25
differentially
expressed
T.
(8.62
%
up-regulated
91.37
down-regulated)
(5.74
94.25
down-regulated)]
by
using
normalized
peptide-spectrum
match
(PSMs)
log2fold
change.
These
similarly
matched
to
carbohydrate
active
family
(CAZymes)
like
glycoside
hydrolase
(GH
family),
carbohydrate-binding
module
(CBM
family)
with
auxiliary
activities,
also
involved
hydrolysis
carbohydrate,
lignin,
xylan,
polysaccharides,
peptides,
oxido-reductase
activity
helps
antioxidant
defense
mechanism.
lignocellulolytic
from
two
different
divisions
proteomics
studies
gave
better
understanding
carbon
recycling
multi-product
lignocellulosic
processes.
Bioresource Technology,
Journal Year:
2024,
Volume and Issue:
408, P. 131164 - 131164
Published: July 26, 2024
Transitioning
away
from
fossil
feedstocks
is
imperative
to
mitigate
climate
change,
and
necessitates
the
utilization
of
renewable,
alternative
carbon
energy
sources
foster
a
circular
economy.
In
this
context,
lignocellulosic
biomass
one-carbon
compounds
emerge
as
promising
that
could
be
renewably
upgraded
by
thermophilic
anaerobes
(thermoanaerobes)
via
gas
fermentation
or
consolidated
bioprocessing
value-added
products.
review,
potential
thermoanaerobes
for
cost-efficient,
effective
sustainable
bioproduction
discussed.
Metabolic
bioprocess
engineering
approaches
are
reviewed
draw
comprehensive
picture
current
developments
future
perspectives
conversion
renewable
chemicals
fuels
interest.
Selected
scenarios
outlined,
offering
practical
insights
into
applicability
at
large
scale.
Collectively,
advantages
regarding
process
economics
facilitate
an
easier
transition
towards
bioprocesses
with
feedstocks.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(1), P. 74 - 74
Published: Jan. 4, 2023
Trichoderma
reesei
is
a
powerful
fungal
cell
factory
for
the
production
of
cellulolytic
enzymes
due
to
its
outstanding
protein
secretion
capacity.
Endoplasmic
reticulum-associated
degradation
(ERAD)
plays
an
integral
role
in
that
responds
pressure
and
removes
misfolded
proteins.
However,
ERAD
growth
endogenous
secretion,
particularly
cellulase
remains
poorly
understood
T.
reesei.
Here,
we
investigated
ability
grow
under
different
stresses
secrete
cellulases
by
disrupting
three
major
genes
(hrd1,
hrd3
der1)
involved
critical
parts
pathway.
Under
ER
stress
induced
high
concentrations
DTT,
knockout
hrd1,
der1
resulted
severely
impaired
growth,
mutants
Δhrd1
Δhrd3
exhibited
sensitivity
wall-disturbing
agents,
CFW
CR.
In
addition,
absence
either
or
led
decreased
heat
tolerance
this
fungus.
These
showed
significant
differences
compared
parental
strain
QM9414.
During
fermentation,
endoglucanase
was
essentially
consistent
with
strain,
while
cellobiohydrolase
β-glucosidase
were
declined.
It
further
discovered
transcription
levels
endoglucanase-encoding
(eg1
eg2)
cellobiohydrolase-encoding
gene
(cbh1)
not
remarkedly
changed.
β-glucosidase-encoding
(bgl1)
significantly
downregulated
ERAD-deficient
mutants,
which
presumably
activation
proposed
feedback
mechanism,
repression
(RESS).
Taken
together,
our
results
indicate
defective
pathway
negatively
affects
provides
novel
insight
into
mechanism
Zeitschrift für Naturforschung C,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 13, 2023
Wood
decomposing
ascomycetes
and
basidiomycetes
group
of
fungi
are
the
most
valuable
microbes
on
earth's
ecosystem
that
recycles
source
carbon;
therefore,
they
essential
for
biorefinery
industries.
To
understand
robustness
enzymes
their
metabolic
pathways
in
fungal
system,
label-free
quantification
total
proteins
was
performed.
The
showed
a
comparable
quantity
protein
abundance
[Trichoderma
citrinoviride
(285),
Thermoascus
aurantiacus
(206),
Ganoderma
lucidum
MDU-7
(102),
G.
(242)].
Differentially
regulated
were
analyzed,
heatmap
shows
upregulated
downregulated
[25
differentially
expressed
T.
(8.62
%
up-regulated
91.37
down-regulated)
(5.74
94.25
down-regulated)]
by
using
normalized
peptide-spectrum
match
(PSMs)
log2fold
change.
These
similarly
matched
to
carbohydrate
active
family
(CAZymes)
like
glycoside
hydrolase
(GH
family),
carbohydrate-binding
module
(CBM
family)
with
auxiliary
activities,
also
involved
hydrolysis
carbohydrate,
lignin,
xylan,
polysaccharides,
peptides,
oxido-reductase
activity
helps
antioxidant
defense
mechanism.
lignocellulolytic
from
two
different
divisions
proteomics
studies
gave
better
understanding
carbon
recycling
multi-product
lignocellulosic
processes.
