Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 4, 2024
Introduction
Furfural,
a
main
inhibitor
produced
during
pretreatment
of
lignocellulose,
has
shown
inhibitory
effects
on
S.
cerevisiae
.
Method
In
the
present
study,
new
strains
named
12–1
with
enhanced
resistance
to
furfural
were
obtained
through
adaptive
laboratory
evolution,
which
exhibited
shortened
lag
phase
by
36
h,
and
an
increased
ethanol
conversion
rate
6.67%
under
4
g/L
furfural.
Results
Discussion
To
further
explore
mechanism
tolerance,
ADR1_1802
mutant
was
constructed
CRISPR/Cas9
technology,
based
whole
genome
re-sequencing
data.
The
results
indicated
that
time
when
begin
grow
20
h
compared
reference
strain
(
CEN.PK113-5D)
g/L.
Additionally,
transcription
levels
GRE2
ADH6
in
ADR1_
1802
53.69
44.95%,
respectively,
according
real-time
fluorescence
quantitative
PCR
analysis.
These
findings
suggest
tolerance
is
due
accelerated
degradation.
Importance:
Renewable
carbon
worldwide
vital
achieve
“zero
carbon”
target.
Bioethanol
from
biomass
one
them.
make
bioethanol
price
competitive
fossil
fuel,
higher
yield
necessary,
therefore,
monosaccharide
should
be
effectively
converted
Saccharomyces
However,
inhibitors
formed
glucose
or
xylose
oxidation
could
lower.
Thus,
tolerant
important
this
process.
As
component
hydrolysate,
shows
obvious
impact
growth
production
get
find
underlying
mechanism,
evolution
technology
applied
study
Molecules,
Journal Year:
2023,
Volume and Issue:
28(13), P. 4990 - 4990
Published: June 25, 2023
β-glucosidases
(BGLs)
play
a
crucial
role
in
the
degradation
of
lignocellulosic
biomass
as
well
industrial
applications
such
pharmaceuticals,
foods,
and
flavors.
However,
application
BGLs
has
been
largely
hindered
by
issues
low
enzyme
activity,
product
inhibition,
stability,
etc.
Many
approaches
have
developed
to
engineer
improve
these
enzymatic
characteristics
facilitate
production.
In
this
article,
we
review
recent
advances
BGL
engineering
field,
including
efforts
from
our
laboratory.
We
summarize
discuss
studies
according
targeted
functions
specific
strategies
used
for
engineering.
ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
13(4), P. 1225 - 1236
Published: March 29, 2024
In
recent
decades,
whole-cell
biocatalysis
has
played
an
increasingly
important
role
in
the
food,
pharmaceutical,
and
energy
sector.
One
promising
application
is
use
of
ethanologenic
yeast
displaying
minicellulosomes
on
cell
surface
to
combine
cellulose
hydrolysis
fermentation
into
a
single
step
for
consolidated
bioprocessing.
However,
cellulosic
ethanol
production
using
existing
biocatalysts
(yWCBs)
not
reached
industrial
feasibility
due
their
inefficient
hydrolysis.
As
prior
studies
have
demonstrated
enzyme
density
yWCB
be
one
most
parameters
enhancing
hydrolysis,
we
sought
maximize
this
parameter
at
both
population
single-cell
levels
yWCBs
tetrafunctional
minicellulosomes.
At
level,
limited
by
presence
nondisplay
constituting
25–50%
all
cells.
study,
identified
cause
plasmid
loss
successfully
eliminated
generate
compositionally
uniform
yWCBs.
demonstrate
that
molecular
crowding,
which
hinders
minicellulosome
assembly.
By
adjusting
integrated
gene
copy
number,
obtained
tunable
display
levels.
This
tunability
allowed
us
avoid
crowding-limited
regime
achieve
maximum
per
cell.
result,
best
strain
showed
cellulose-to-ethanol
yield
4.92
g/g,
corresponding
96%
theoretical
near-complete
conversion
(∼96%)
starting
(1%
PASC).
Our
holistic
engineering
strategy
combines
level
approach
broadly
applicable
enhance
WCB
performance
other
biocatalytic
cascade
schemes.
Green Chemistry,
Journal Year:
2023,
Volume and Issue:
25(5), P. 2011 - 2020
Published: Jan. 1, 2023
A
simplified
aerobic
fungal
and
facultative
bacterial
consortium
can
co-exist
owing
to
the
different
oxygen
spatial
niches
created
by
biofilm
system,
achieving
efficient
lactic
acid
production
from
cellulose
via
CBP.
Journal of Fungi,
Journal Year:
2022,
Volume and Issue:
8(7), P. 752 - 752
Published: July 20, 2022
Since
molecular
biology
and
advanced
genetic
techniques
have
become
important
tools
in
a
variety
of
fields
interest,
including
taxonomy,
identification,
classification,
possible
production
substances
proteins,
applications
pharmacology,
medicine,
the
food
industry,
there
has
been
significant
progress
studying
yeast
genome
its
potential
applications.
Because
this
potential,
as
well
their
manageability,
safety,
ease
cultivation,
reproduction,
yeasts
are
now
being
extensively
researched
order
to
evaluate
growing
number
natural
sustainable
provide
many
benefits
humans.
This
review
will
describe
what
are,
how
they
classified,
attempt
rapid
overview
current
future
yeasts.
The
then
discuss
yeasts—including
those
molecularly
modified—are
used
produce
biofuels,
proteins
such
insulin,
vaccines,
probiotics,
beverage
preparations,
additives
could
be
environmental
bioremediation
biocontrol
for
plant
infections.
does
not
delve
into
issues
raised
during
studies
research,
but
rather
presents
positive
outcomes
that
enabled
several
industrial,
clinical,
agricultural
past
future,
most
recent
on
cow-free
milk.
