Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 20, 2024
The
recognition
of
small
molecules
plays
a
crucial
role
in
disease
diagnosis,
environmental
assessment,
and
food
safety.
Currently,
their
elements
predominantly
rely
on
antibodies
aptamers
while
suffering
from
limitation
the
complex
screening
process
due
to
low
immunogenicity
molecules.
Herein,
we
present
top-down
computational
design
strategy
for
molecule
peptides
(MRPs)
enzyme-peptide
self-assembly
chemiluminescence
biosensing.
Taking
ochratoxin
A
(OTA)
as
an
illustrative
example,
human
serum
albumin
(HSA)
was
selected
parental
protein
its
high
affinity
OTA
binding.
Through
iterative
simulations
involving
binding
domain
HSA-OTA
complex,
our
identified
specific
15-mer
MRP
(RLKCASLKFGERAFK),
which
possesses
excellent
(38.02
±
1.24
nM)
against
OTA.
Molecular
dynamics
revealed
that
unfolds
into
flexible
short
chain
with
OTA,
but
exhibits
weak
or
no
five
structurally
similar
mycotoxins.
Furthermore,
developed
novel
approach
mediated
by
calcium(II)
obtain
nanoflowers,
integrates
both
element
(MRP)
signal
translator
(enzyme)
assembled
nanoflowers
allow
MRPs
be
directly
utilized
tracer
biosensing
without
labeling
secondary
antibodies.
This
computational-to-application
offers
new
route
small-molecule
recognition.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: April 24, 2025
Melatonin
is
a
key
hormone
that
regulates
the
sleep-wake
cycle
and
plays
an
important
role
in
maintaining
circadian
rhythm
sleep
onset.
The
daily
rise
melatonin
secretion
associated
with
increased
tendency
to
sleep,
occurring
approximately
2
h
before
bedtime.
This
correlation
between
levels
onset
makes
it
reliable
biomarker
for
disorders.
An
accurate
assessment
of
dim
light
(DLMO)
vital
understanding
timing
diagnosing
disruptions.
However,
traditional
methods
detecting
saliva
are
either
complex
or
lack
sensitivity
required
DLMO,
especially
at
low
concentrations.
Here,
we
present
novel
competitive
enzyme-linked
aptamer-based
assay
developed
detect
saliva.
Unlike
conventional
assays,
this
technique
utilizes
chemically
synthesized
single-stranded
DNA
RNA
aptamers,
which
bind
high
specificity
sensitivity.
measures
melatonin,
attaining
linear
dynamic
range
from
8.62
×
10‒6
M
3.9
10‒11
M,
detection
limit
2.5
10‒12
(~
0.57
pg/mL).
Additionally,
aptamer
showed
small
binding
its
counter
targets
acceptable
recovery
when
spiked
four
times
diluted
buffer.
Overall,
portrayed
potential
aptamers
could
be
beneficial
accurately
determining
particularly
individuals
very
levels,
such
as
elderly
those
neurodegenerative
conditions.
Determining
precise
measurement
DLMO
will
facilitate
diagnosis
disruption,
enabling
healthcare
providers
optimize
selection
therapeutic
behavioural
interventions
tailored
individual's
unique
rhythm.
Cogitare Enfermagem,
Journal Year:
2025,
Volume and Issue:
30
Published: Jan. 1, 2025
Resumo
Objetivo:
identificar
a
presença
de
adoecimento
mental
e
as
estratégias
enfrentamento
utilizadas
pelos
enfermeiros
um
hospital
universitário
localizado
na
cidade
Maceió,
Alagoas,
Brasil.
Método:
estudo
quantitativo,
transversal,
do
tipo
descritivo,
realizado
entre
junho
2022
janeiro
2023.
Foram
utilizados:
Questionário
sociodemográfico
aspectos
da
saúde,
Escala
Avaliação
Sintomas-40-R
Inventário
Estratégias
Enfrentamento
Folkman
Lazarus.
Realizada
análise
estatística
descritiva
univariada
coeficiente
correlação
Pearson.
Resultados:
foram
entrevistadas
97
pessoas
que
relataram
já
se
sentiram
deprimidas
por
duas
semanas
ou
mais
(34%);
tinham
diagnóstico
transtorno
(35,1%);
estão
fazendo
acompanhamento
psicológico
(8,2%)
psiquiátrico
(7,2%).
Apresentaram
maior
comprometimento
dimensão
obsessividade-compulsividade
utilizaram
estratégia
Reavaliação
Positiva.
Conclusão:
o
contribui
para
orientar
gestores
enfermagem
implementação
programas
incentivem
visando
melhorar
saúde
desses
profissionais.
Cogitare Enfermagem,
Journal Year:
2025,
Volume and Issue:
30
Published: Jan. 1, 2025
Resumen
Objetivo:
identificar
la
presencia
de
enfermedad
mental
y
las
estrategias
afrontamiento
utilizadas
por
enfermeros
un
hospital
universitario
localizado
en
ciudad
Maceió,
Alagoas,
Brasil.
Método:
estudio
cuantitativo,
transversal,
descriptivo,
realizado
entre
junio
2022
enero
2023.
Se
utilizaron:
Cuestionario
sociodemográfico
aspectos
salud,
Escala
Evaluación
Síntomas-40-R
e
Inventario
Estrategias
Afrontamiento
Folkman
Lazarus.
analizó
estadística
descriptiva
univariante
coeficiente
correlación
Pearson.
Resultados:
se
entrevistó
a
97
personas
que
declararon
haberse
sentido
deprimidas
durante
quince
días
o
más
(34%);
haber
sido
diagnosticadas
trastorno
(35,1%);
estar
recibiendo
asesoramiento
psicológico
(8,2%)
psiquiátrico
(7,2%).
Mostraron
mayor
deterioro
dimensión
obsesividad-compulsividad
hicieron
uso
estrategia
Reevaluación
positiva.
Conclusión:
el
ayuda
orientar
los
gestores
enfermería
implantación
programas
fomenten
dirigidas
mejorar
salud
estos
profesionales.
Cogitare Enfermagem,
Journal Year:
2025,
Volume and Issue:
30
Published: Jan. 1, 2025
Abstract
Objective:
to
identify
the
presence
of
mental
illness
and
coping
strategies
nurses
use
at
a
university
hospital
in
Maceió,
Alagoas,
Brazil.
Method:
quantitative,
cross-sectional,
descriptive
study
between
June
2022
January
2023.
The
following
were
used:
Sociodemographic
health
aspects
questionnaire,
Symptom
Assessment
Scale-40-R,
Folkman
Lazarus
Coping
Strategies
Inventory.
Univariate
statistics
Pearson’s
correlation
coefficient
analyzed.
Results:
97
people
interviewed
who
reported
that
they
had
already
felt
depressed
for
two
weeks
or
more
(34%),
been
diagnosed
with
disorder
(35.1%),
undergoing
psychological
(8.2%)
psychiatric
(7.2%)
counseling.
They
showed
significant
impairment
obsessiveness-compulsiveness
dimension
made
greater
strategy
Positive
Reappraisal.
Conclusion:
helps
guide
nursing
managers
implementing
programs
encourage
aimed
improving
these
professionals.
Life,
Journal Year:
2025,
Volume and Issue:
15(5), P. 715 - 715
Published: April 28, 2025
Circadian
rhythms,
essential
for
regulating
human
physiology
and
behavior,
are
influenced
by
light
exposure,
particularly
at
night.
This
study
examined
the
impact
of
red
(631
nm)
blue
(464
LED
on
melatonin
secretion,
a
key
circadian
marker.
Twelve
participants
aged
19–55
years
were
exposed
to
three
hours
(9:00
p.m.–midnight),
with
hourly
saliva
samples
analyzed
via
ELISA
track
levels.
Initially,
levels
comparable
under
both
conditions.
After
one
hour,
lights
suppressed
melatonin,
but
differences
emerged
after
two
hours:
light-maintained
suppression,
7.5
pg/mL,
while
allowed
recovery
26.0
pg/mL
(p
=
0.019).
pattern
persisted
third
hour.
Blue
had
stronger
suppression
effects,
in
younger
men.
These
results
underscore
light’s
disruptive
effects
health
highlight
as
less
alternative
nighttime
environments.
JDR Clinical & Translational Research,
Journal Year:
2022,
Volume and Issue:
7(1_suppl), P. 16S - 24S
Published: Sept. 19, 2022
Change
in
our
world
is
happening
quickly.
For
dentistry,
this
no
exception,
and
now
the
time
to
foster
new
opportunities.
Advances
foundational
science
of
dental,
oral,
craniofacial
health
are
expanding
enabling
personalized
care
built
on
strong
discoveries
fields
microbiology,
immunology,
neuroscience.
Digital
communication
through
electronic
records,
patient
portals,
teledentistry
emerging
as
vital
armamentarium
for
contemporary
dental
practice.
Wearables
linked
with
smartphone
apps
trending
monitor
pain
levels,
postoperative
symptoms,
wound
healing,
home
oral
habits.
Oral
fluid
diagnostics
rapidly
surfacing
use
diagnostics,
drug
testing,
hormone
detection.
Manipulation
microbiome
under
investigation
caries
management
potential
impact
systemic
conditions.
Resolving
mediators
inflammation
promising
addressing
chronic
that
plagues
periodontal
patients.
New
imaging
modalities
paired
digital
approaches
3-dimensional
printing
formulating
disruptions
technical
aspects
dentistry.
Augmented
reality
virtual
providing
great
teaching
tools
students
bear
promise
lifelong
learning
providers.
Optimization
information
technology
incorporation
data
partnered
artificial
intelligence
machine
improve
a
system
approach
will
benefit
large
numbers
These
exciting
times
profession,
yet
we
need
navigate
these
paths
carefully.
Technology
needs
significantly
positively
change
experience
those
involved
(patients,
dentists,
staff,
payors).
technologies
be
backed
solid
research
implementation
science.
Automation
should
focus
improving
attention
underserved
not
lose
critical
human
connection
patients
need.
There
innovations
also
responsibility
ensure
they
evidence
based
rigorous
deployable
equity
sensitivity.
Knowledge
Transfer
Statement:
This
article
discusses
treatments
have
enormous
revolutionize
care,
including
novel
concepts
between
dentists
patients,
biologics
around
diagnosis
treatment,
and,
finally,
real-time
optimization
technology.
The
early
validation
can
drive
down
their
costs
provide
better
medical
services
all
members
society.
Nucleic Acids Research,
Journal Year:
2023,
Volume and Issue:
52(1), P. 73 - 86
Published: Nov. 22, 2023
Abstract
Beyond
storage
and
transmission
of
genetic
information
in
cellular
life,
nucleic
acids
can
perform
diverse
interesting
functions,
including
specific
target
recognition
biochemical
reaction
acceleration;
the
versatile
biopolymers,
however,
are
acutely
vulnerable
to
hydrolysis-driven
degradation.
Here,
we
demonstrate
that
cage
effect
choline
dihydrogen
phosphate
permits
active
folding
like
water,
but
prevents
their
phosphodiester
hydrolysis
unlike
water.
The
choline-based
ionic
liquid
not
only
serves
as
a
universal
inhibitor
nucleases,
exceptionally
extending
half-lives
up
6
500
000
times,
highly
useful
tasks
(e.g.
mRNA
detection
molecular
beacons,
ligand
aptamers,
transesterification
ribozymes)
be
also
conducted
with
well-conserved
affinities
specificities.
As
liberated
from
function
loss
degradation
risk,
presence
undesired
unknown
nucleases
does
undermine
desired
functions
without
artifacts
even
nuclease
cocktails
human
saliva.
