Metabolic Engineering of Saccharomyces cerevisiae for High Bioproduction of (+)-Nootkatone

ACS Agricultural Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Language: Английский

---

Fusion of enzymatic proteins: Enhancing biological activities and facilitating biological modifications

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 340, P. 103473 - 103473

Published: March 9, 2025

Language: Английский

---

Chemoenzymatic synthesis

Communications Chemistry, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 13, 2025

Communications Chemistry is pleased to introduce a Collection of research works focused on recent developments within the interdisciplinary field chemoenzymatic synthesis. Here, Guest Editors highlight key themes and look towards future this field.

Language: Английский

---

Peptide bridging for cofactor channeling in fusion enzyme lowers cofactor input by two orders of magnitude

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION), Journal Year: 2025, Volume and Issue: 71, P. 390 - 403

Published: April 1, 2025

Language: Английский

---

A fused hybrid enzyme of 8-hydroxygeraniol oxidoreductase (8HGO) from Gardenia jasminoides and iridoid synthase (ISY) from Catharanthus roseus significantly enhances nepetalactol and iridoid production

Planta, Journal Year: 2024, Volume and Issue: 259(3)

Published: Feb. 6, 2024

Language: Английский

---

Microbial β-glucosidases: Recent advances and applications

Biochimie, Journal Year: 2024, Volume and Issue: 225, P. 49 - 67

Published: May 9, 2024

The global β-glucosidase market is currently estimated at ∼400 million USD, and it expected to double in the next six years; a trend that mainly ascribed demand for enzyme biofuel processing. Microbial β-glucosidase, particularly, has thus garnered significant attention due its ease of production, catalytic efficiency, versatility, which have all facilitated biotechnological potential across different industries. Hence, there are continued efforts screen, produce, purify, characterize evaluate industrial applicability from actinomycetes, bacteria, fungi, yeasts. With this rising various cost-effective efficient approaches being explored discover, redesign, enhance their production functional properties. Thus, present review provides an up-to-date overview advancements utilization microbial β-glucosidases as "Emerging Green Tools" 21st-century In regard, focus was placed on use recombinant technology, protein engineering, immobilization techniques targeted improving enzyme. Furthermore, insights were given into recent progress made conventional applications, well current commercial status-with patents.

Language: Английский

---

Enzyme Conjugation - A Promising Tool for Bio-catalytic and Biotransformation Applications – A Review

Topics in Catalysis, Journal Year: 2024, Volume and Issue: unknown

Published: July 4, 2024

Language: Английский

---

Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris

Bioengineering, Journal Year: 2025, Volume and Issue: 12(1), P. 68 - 68

Published: Jan. 14, 2025

Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability high-quality enzymes associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression have been studied meeting demand. Among these systems, Pichia pastoris system has emerged widely used platform producing secreted proteins. This article provides an overview studies involving recombinant polymer-degrading using P. system. Research interested with depolymerising ability, including cutinase, lipase, laccase, are highlighted in review. key factors influencing discussed, shedding light challenges opportunities biocatalysts through

Language: Английский

---

Construction of a Plasmid-Free Escherichia coli Strain with Enhanced Heme Supply to Produce Active Hemoglobins

Metabolites, Journal Year: 2025, Volume and Issue: 15(3), P. 151 - 151

Published: Feb. 23, 2025

Background: Heme is an important cofactor and plays crucial roles in the correct folding of hemoproteins. The synthesis heme can be enhanced by plasmid-based expression biosynthetic genes. However, genetically unstable requires utilization antibiotics to maintain high copy numbers plasmids. Methods: rate-limiting steps biosynthesis were first analyzed based on previous studies accumulation intermediates was achieved adding precursor (5-aminolevulinic acid, ALA). Next, intracellular porphyrin increased deleting transporter TolC. Finally, synthetic genes modified integrating hemA hemL into cheW yciQ locus, assembling enzymes HemC HemD with RIAD-RIDD tags, replacing promoters hemE/hemH constitutive promoter PJ23100, degradation gene yfeX. Results: An supply HEME2 strain obtained a titer 0.14 mg/L, which 4.60-fold higher than that C41(DE3) strain. applied produce human hemoglobin leghemoglobin. peroxidase activity 1.29-fold 42.4% HEME2-hHb values control C41-hHb. In addition, content leghemoglobin 39.2% 53.4% HEME2-sHb compared C41-sHb. Conclusions: A plasmid-free Escherichia coli capable efficient stable constructed used for production high-active hemoglobins.

Language: Английский

---

Enhancing the expression of chondroitin 4-O-sulfotransferase for one-pot enzymatic synthesis of chondroitin sulfate A

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 337, P. 122158 - 122158

Published: April 12, 2024

Language: Английский

---