The Ying and Yang of Ganglioside Function in Cancer

Cancers, Journal Year: 2023, Volume and Issue: 15(22), P. 5362 - 5362

Published: Nov. 10, 2023

The plethora of information about the expression cancer cell-associated gangliosides, their role(s) in signal transduction, and potential usefulness development treatments makes this an appropriate time to review these enigmatic glycosphingolipids. Evidence, reflecting work many, indicates that (1) specific not generally found high concentrations most normal human cells, can be linked certain types cancer. (2) Gangliosides affect ability cells interact either directly or indirectly with growth factor receptors, thereby changing such things as a cell’s mobility, rate proliferation, metastatic ability. (3) Anti-ganglioside antibodies have been tested, some success, for cancers. (4) Cancer-associated gangliosides shed into circulation (a) immune cell responsiveness positively negatively, (b) considered diagnostic markers, (c) used look recurrence. (5) Cancer registries enable investigators evaluate data from sufficient numbers patients obtain therapies. Despite advances made, discussion possible approaches identifying additional treatment strategies inhibit metastasis, responsible majority deaths patients, well treating therapy-resistant tumors, is included.

Language: Английский

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Targeting HER2 and FGFR-positive cancer cells with a bispecific cytotoxic conjugate combining anti-HER2 Affibody and FGF2

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127657 - 127657

Published: Oct. 28, 2023

Breast cancer remains a significant global health challenge, necessitating the development of effective targeted therapies. This study aimed to create bispecific targeting molecules against HER2 and FGFR1, two receptors known play crucial roles in breast progression. By combining high-affinity Affibody Z

Language: Английский

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1,6-Thiol Addition of Trifunctional N-Alkylpyridinium for Site-selective Dual Functionalization of Proteins

Published: April 22, 2024

We report N-alkylpyridinium reagents for site-selective dual modification of proteins via a regioselective 1,6-thiol addition reaction. The derivatives can be syn-thesized in two reaction steps and demonstrate high labelling efficiency chemose-lectivity towards cysteine residues. This is combined with strain-promoted azide-alkyne click inverse-electron-demand Diels−Alder reactions to achieve functionalization sequential one-pot A Rho-inhibiting enzyme functionalized cancer cell-targeting peptide fluorescent dye successful vitro uptake imaging concomitant inhibition specific Rho-mediated cellular pathways. ease synthesis, fast kinetics, solubility regioselectivity make unique protein/peptide increase their functional diversities medical applications.

Language: Английский

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Engineered intrinsically fluorescent galectin-8 variants with altered valency, ligand recognition and biological activity

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134371 - 134371

Published: July 31, 2024

Galectin-8 is a small soluble lectin with two carbohydrate recognition domains (CRDs). N- and C-terminal CRDs of Gal-8 differ in their specificity for glycan ligands. Here, we wanted to find out whether oligomerization individual galectin-8 affects its biological activity. Using green fluorescent protein polygons (GFPp) as an scaffold, generated intrinsically altered valency. We show that oligomers C-CRD are characterized by significant cell surface affinity. Furthermore, the multivalency resulting variants has impact on cellular activities such signaling, heparin binding proliferation. Our data indicates tunable valence useful tool modifying activity galectins.

Language: Английский

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Thermoresponsive [(PLA)-b-(Car)] based frustum-shaped capsules with extended porosity for biosensing and biomedical applications

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Abstract We present, a facile method for the synthesis of block co-polymer [(PLA)-b-(Car)], (PLA-Poly Lactic acid and Car-Carbazole) followed by development cone ice-cream cup-shaped frustums with nanoporous capsules bio-sensing drug loading applications. Thermoplastic polyester PLA aminated an aromatic heterocyclic organic compound Car gives desired shape characteristics extended porosity on surface. The size, shape, morphology are tunable concerning solvents thermal treatment. Interestingly, hollow (half-broken coconut type) has been carried out without using any precursors surfactants. Synthesis [(PLA)-b-(Car)] confirmed through Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR; 1H 13C). However, surface properties were field emission scanning electron microscopy (FE-SEM), Bruner Emmett Teller (BET), atomic force (AFM) characterizations. capsule size ranges from ~200 nm to 1 µm (in diameter) scales ~60 80 nm. porous biosensing biomedical applications can be considered as unique novel characteristic property our invention. This kind ice cream used stimuli-responsive catalytic activity fluorescence measurement cysteine gold nanoparticle [(Cys)-(Au+)] enhanced payload

Language: Английский

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Intracellular delivery strategies using membrane-interacting peptides and proteins

Nanoscale, Journal Year: 2024, Volume and Issue: 16(33), P. 15465 - 15480

Published: Jan. 1, 2024

Cytosolic delivery of therapeutic cargo mediated by membrane interacting peptides and proteins. Created with BioRender.com.

Language: Английский

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Intranasal trivalent candidate vaccine induces strong mucosal and systemic immune responses against Neisseria gonorrhoeae

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 26, 2024

The spread of multidrug-resistant strains

Language: Английский

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Binding Strength, Not Valency, Dictates Accumulation and Penetration of Affinity Targeted Macromolecules into Solid Tumors

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 503 - 513

Published: Dec. 27, 2024

The efficacy of tumor-targeted therapeutics, engineered to engage specific cellular receptors promote accumulation and penetration, is strongly influenced by the carrier's affinity for its target valency binding molecules incorporated into carrier. Previous research has primarily focused on improving targeting augmenting number proteins carrier, inadvertently raising avidity without isolating individual effects strength valency. Herein, we precisely evaluate impact multivalency tumor with a recombinant approach independently control valency, avidity, size. Our findings reveal that constructs equivalent exhibit comparable receptor engagement extravasation, regardless Moreover, excessive adversely affected highest-avidity construct showing diminished exposure. These results indicate overall strength, not primary determinant targeting, providing valuable insights designing effective macromolecular drug carriers.

Language: Английский

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Thermoresponsive [(PLA)-co-(Car)] based frustum-shaped capsules with extended porosity for biosensing and biomedical applications

Journal of Porous Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Language: Английский

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