Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
11
Published: Nov. 15, 2023
Soil
and
water
contamination
due
to
organic
or
pollutants
has
long
been
a
major
19
environmental
problem
is
attracting
increasing
attention
(Hu
et
al.,
2023;Thacharodi
2023).
20Different
have
reported
be
detrimental
public
health.
For
example,
crude
oil
21
occurred
during
the
processes
of
petroleum
exploration,
transportation,
storage,
22
refining.
Similarly,
many
other
chemicals
(i.e.,
polychlorinated
biphenyl
(PCBs),
polycyclic
aromatic
23
hydrocarbons
(PAHs),
pesticides,
etc.),
synthetic
plastics,
pharmaceuticals
in
wastewaters
are
not
24
completely
removed
by
conventional
treatment
plants
(
All
claims
expressed
this
article
solely
those
authors
do
necessarily
represent
92
their
affiliated
organizations,
publisher,
editors
reviewers.
Any
93
product
that
evaluated
claim
may
made
its
manufacturer,
94
guaranteed
endorsed
publisher.
95
96
Biotechnology Advances,
Journal Year:
2024,
Volume and Issue:
77, P. 108438 - 108438
Published: Aug. 30, 2024
The
transition
to
renewable
energies
and
electric
vehicles
has
triggered
an
unprecedented
demand
for
metals.
Sustainable
development
of
these
technologies
relies
on
effectively
managing
the
lifecycle
critical
raw
materials,
including
their
responsible
sourcing,
efficient
use,
recycling.
Metal
recycling
from
electronic
waste
(e-waste)
is
paramount
importance
owing
ore-exceeding
amounts
elements
high
toxicity
heavy
metals
organic
pollutants
in
e-waste
natural
ecosystem
human
body.
Heterotrophic
microbes
secrete
numerous
metal-binding
biomolecules
such
as
acids,
amino
cyanide,
siderophores,
peptides,
biosurfactants
which
can
be
utilized
eco-friendly
profitable
metal
In
this
review
paper,
we
presented
a
heterotrophic
organisms
biomining,
current
barriers
hampering
industrial
application
acid
bioleaching
biocyanide
leaching.
We
also
discussed
how
challenges
surmounted
with
simple
methods
(e.g.,
culture
media
optimization,
separation
microbial
growth
extraction
process)
state-of-the-art
biological
approaches
artificial
community,
synthetic
biology,
metabolic
engineering,
advanced
fermentation
strategies,
biofilm
engineering).
Lastly,
showcased
emerging
artificially
synthesized
biosurfactants)
derived
heterotrophs
potential
inexpensive,
low-impact,
selective
recovery
solutions.
Biotechnology and Bioengineering,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 16, 2025
The
bioaugmentation
performance
is
severely
reduced
in
the
treatment
of
high-saline
pesticide
wastewater
because
growth
and
degradation
activity
degraders
are
significantly
inhibited
by
high
salt
concentrations.
In
this
study,
a
heterologous
biodegradation
pathway
comprising
seven
genes
mpd/pnpABCDEF
responsible
for
bioconversion
p-nitrophenol
(PNP)-substituted
organophosphorus
pesticides
(OPs)
into
β-oxoadipate
encoding
Vitreoscilla
hemoglobin
(VHb)
green
fluorescent
protein
(GFP)
were
integrated
genome
salt-tolerant
chassis
Halomonas
cupida
J9,
to
generate
genetically
engineered
halotolerant
degrader
J9U-MP.
RT-PCR
assays
demonstrated
that
nine
exogenous
successfully
transcribed
mRNA
Gas
chromatography
analysis
methyl
parathion
(MP)
its
intermediates
expressed
MP
hydrolase
PNP-degrading
enzymes
PnpABCD
show
obvious
toward
specific
substrates
Stable
isotope
showed
J9U-MP
able
efficiently
convert
13C6-PNP
13CO2,
demonstrating
complete
mineralization
high-salt
media.
stable
during
passage
culture,
genomic
integration
does
not
negatively
influence
Under
oxygen-limited
conditions,
VHb-expressing
inhibition
significant
reduction
rate.
A
real-time
monitoring
system
with
enhanced
GFP
used
track
motion
bioremediation.
Moreover,
50
mg/L
(i.e.,
PNP
HQ)
completely
degraded
within
12
h
supplemented
60
g/L
NaCl.
After
3
days
incubation,
25
was
converted
13CO2
Our
results
highlight
power
synthetic
biology
creating
new
pollutant-mineralizing
strains.
strong
competitive
advantages
high-salinity
low-oxygen
environments
make
suitable
situ
OP
wastewater.
Frontiers in Plant Science,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 22, 2025
With
the
advancement
of
plant
synthetic
biology,
complex
genetic
engineering
circuits
are
being
developed,
which
require
more
diverse
regulatory
elements
(promoters)
to
operate.
Constitutive
promoters
widely
used
for
such
gene
projects,
but
list
strong,
constitutive
with
strength
surpassing
promoter,
CaMV35S,
is
limited.
In
this
work,
we
attempted
increase
promoter
library
by
developing
efficient
suitable
high-level
expression.
To
do
that,
selected
three
strong
pararetroviral-based
from
Mirabilis
mosaic
virus
(MMV),
Figwort
(FMV),
and
Horseradish
latent
(HRLV)
rationally
designed
combined
their
elements.
We
then
tested
newly
developed
in
Nicotiana
benthamiana
found
a
highly
active
tri-hybrid
MuasFuasH17
(MFH17).
further
these
generating
random
mutant
DNA
shuffling
techniques
an
attempt
change/improve
MFH17
promoter.
evaluated
activity
Oryza
sativa
seedlings
studied
effect
as-1
present
it.
Finally,
efficacy
tissue
specificity
planta
transgenic
tabacum
Arabidopsis
thaliana
plants
it
driving
throughout
tissues.
Overall,
conclude
that
tripartite
constitutive,
dual-species
(dicot
monocot)
expressing
can
be
valuable
addition
biology.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 29, 2025
Abstract
The
inoculation
of
microbes
into
soil
environments
has
numerous
applications
for
improving
quality
and
crop
health;
however,
the
ability
exogenous
engineered
to
survive
spread
in
remains
uncertain.
To
address
this
challenge,
we
assayed
survival
Mycobacterium
smegmatis
,
with
either
plasmid
transformation
or
genome
integration,
as
well
its
mycobacteriophage
Kampy,
both
sterilized
non-sterilized
microcosms
over
a
period
49
days.
While
M.
Kampy
persisted
all
microcosms,
there
was
minimal
evidence
5
cm
away
from
site.
There
higher
prevalence
observed
than
soil,
suggesting
detrimental
effect
native
biotic
viral
community
on
phage
proliferate
microcosm.
Additionally,
abundance
genome-integrated
bacteria
relative
plasmid-carrying
loss
duration
experiment
suggest
burden
associated
harboring
plasmids,
although
plasmids
were
still
retained
across
our
knowledge,
is
first
study
simultaneously
measure
persistence
their
employing
plasmid-based
circuits.
As
such,
provides
novel
understanding
challenges
deployment
bioengineered
environments.
Importance
Healthy
essential
sustain
life,
it
habitable
land,
enables
food
production,
promotes
biodiversity,
sequesters
cycles
nutrients,
filters
water.
Given
degradation,
treatment
that
promote
health
could
improve
global
sustainability;
furthermore,
application
bioengineering
synthetic
biology
these
allows
fine-tunable
robust
control
gene-of-interest
expression.
These
solutions
require
successful
an
environment
which
abundant
competition
often
limited
nutrients
can
result
bacterial
death
dormancy.
This
employs
chassis
alongside
bacteriophage
assess
non-native
soil.
Insights
highlight
important
must
be
overcome
field.
