Sinensetin suppresses breast cancer cell progression via Wnt/β-catenin pathway inhibition

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(1), P. 348 - 362

Published: Jan. 1, 2024

Background: Although there are many treatments for breast cancer, such as surgery, radiotherapy, chemotherapy, estrogen receptor antagonists, immune checkpoint inhibitors and so on. However, safer more effective therapeutic drugs cancer needed. Sinensetin, a drugs, come from citrus species medicinal plants used in traditional medicine, while its role underlying mechanism remain unclear. Our study aimed to investigate the of sinensetin cancer. Methods: Cell Counting Kit-8 (CCK-8) was determine safe concentration MCF-10A, MCF7 MDA-MB-231 cells; 120 μM subsequent experiments. Real time polymerase chain reaction (RT-PCR), Western blotting, Terminal Deoxynucleotidyl Transferase mediated dUTP Nick-End Labeling (TUNEL) apoptosis assay, Transwell invasion assay Clone formation were this cell viability, mRNA expression, protein levels, apoptosis, proliferation, Results: Herein, our results showed that suppressed viability promoted cells. Treatment with µM 24 h no significant toxicity normal mammary decreased invasion, epithelial-mesenchymal transition (EMT), downregulated β-catenin, lymphatic enhancing factor 1 (LEF1), T-cell (TCF) 1/TCF7, TCF3/TCF7L1 expression The Wnt agonist SKL2001 reversed inhibitory effect on survival, metastasis, EMT. Sinensetin-induced downregulation LEF1, TCF1/TCF7 upregulated by Conclusions: In summary, metastasis via inhibition Wnt/β-catenin pathway adverse effects confirmed cells provided better understanding mechanism.

Language: Английский

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Biosynthesis and bioactivities of triterpenoids from Centella asiatica: Challenges and opportunities

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108541 - 108541

Published: Feb. 1, 2025

Language: Английский

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A comprehensive review on the potential applications of medicine Polygonatum species in the food sector

Food Bioscience, Journal Year: 2024, Volume and Issue: 60, P. 104116 - 104116

Published: April 17, 2024

Language: Английский

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Plant hairy roots: Induction, applications, limitations and prospects

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 219, P. 119104 - 119104

Published: July 2, 2024

Language: Английский

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De novo biosynthesis of dihydroquercetin in an engineered Yarrowia lipolytica with enhanced (2S)-eriodictyol

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105912 - 105912

Published: Jan. 1, 2025

Language: Английский

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Metabolic engineering strategies for food additive ferulic acid production

Food Bioscience, Journal Year: 2025, Volume and Issue: 65, P. 106108 - 106108

Published: Feb. 10, 2025

Language: Английский

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Recent Advancements in the Biomanufacturing of Crocetin and Crocins: Key Enzymes and Metabolic Engineering

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Crocetin and crocins are high-value apocarotenoids recognized for their role as food colorants well numerous industrial therapeutic applications. Biotechnological platforms have the potential to replace traditional plant-based extraction of these compounds with a more sustainable approach. This review first introduced catalytic characteristics key enzymes involved in biosynthetic pathway crocetin crocins, including carotenoid cleavage dioxygenases, aldehyde dehydrogenases, uridine diphosphate glycosyltransferases. Next, we highlighted advanced metabolic engineering strategies aimed at enhancing crocin production, such increasing pool precursors cofactors, protein mining engineering, tuning expression, biosensor, genomic integration, process optimization. Finally, paper proposed tools associated further boosting heterologous production meet commercial-scale demands.

Language: Английский

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Biochemical evaluation of molecular parts for flavonoid production using plant synthetic biology

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 15, 2025

Among organisms on Earth, plants have the unique ability to produce a wide variety of biomolecules using soil nutrients, air, and solar energy. Therefore, are regarded as most productive cost-efficient bioreactors among living organisms. Flavonoids, major group secondary metabolites exclusively produced in plants, play crucial roles plant physiology various effects human health. Flavonoids used diverse industries such pharmaceutical, nutraceutical, cosmetics industries. These compounds typically extracted from specific that naturally large amounts target flavonoid for commercial production. However, with increasing demand flavonoids, efforts been made enhance production synthetic biology sustainable microbes or plants. Synthetic has utilized metabolic engineering reconstitute biosynthetic pathways flavonoids at whole-pathway level, thereby enhancing For efficient biology, first all, optimized molecular parts enzymes must be identified selected. The best modules precursors final can then constructed these parts. In this review, we summarize enzyme kinetics natural engineered derived different species provide insight into selection parts, design devices, reconstitution based performance biology.

Language: Английский

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Customized anion-exchange covalent organic frameworks for efficient extraction of natural products

Separation and Purification Technology, Journal Year: 2024, Volume and Issue: 342, P. 127072 - 127072

Published: March 11, 2024

Language: Английский

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A comprehensive review of the molecular and genetic mechanisms underlying gum and resin synthesis in Ferula species

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 269, P. 132168 - 132168

Published: May 8, 2024

Language: Английский

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