Supercritical Fluid Extraction of Coumarins and Flavonoids from Citrus Peel

The Journal of Supercritical Fluids, Journal Year: 2024, Volume and Issue: 215, P. 106396 - 106396

Published: Sept. 6, 2024

Language: Английский

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LncRNA TCL6 regulates miR-876–5p/MYL2 axis to suppress breast cancer progression

Translational Oncology, Journal Year: 2025, Volume and Issue: 53, P. 102210 - 102210

Published: Jan. 27, 2025

Language: Английский

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Citrus flavonoids for overcoming breast cancer resistance to methotrexate: identification of potential targets of nobiletin and sinensetin

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 20, 2025

Breast cancer is a potentially fatal illness that affects millions of women worldwide. Methotrexate (MTX) may be beneficial for treating breast cancer; however, high doses and prolonged use can cause drug resistance. Although certain citrus flavonoids—nobiletin, sinensetin, tangeretin, hesperidin, hesperetin, naringenin—may overcome resistance to chemotherapy, no study has investigated MTX This the potential natural chemicals, specifically nobiletin in cells using MTX-resistant MCF-7 (MCF-7/MTX) cells. Protein targets flavonoids were identified from multiple databases collected Venny 2.1. Microarray data MCF-7/MTX acquired Gene Expression Omnibus. Subsequently, we constructed protein–protein interaction network selected hub proteins. ontology Kyoto Encyclopedia Genes Genomes pathway enrichment analysis, drug– disease–gene analyses, genetic alteration examination, receiver operating characteristic curve mRNA levels prognostic value molecular docking analysis performed along with vitro experiments. Cytotoxicity (individually combined) was assessed Nobiletin sinensetin significantly enhanced cytotoxicity BCL2L1, CDK1, EGFR, PTGS2, PLK1, MMP2, ACHE, ABCG2, KIT genes enriched cholinesterase activity, cell cycle regulation, PI3K/Akt signaling pathway. impeded ACHE activities based on docking. combination MTX.

Language: Английский

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Role of PARP Inhibitors: A New Hope for Breast Cancer Therapy

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2773 - 2773

Published: March 19, 2025

Tumors formed by the unchecked growth of breast cells are known as cancer. The second most frequent cancer in world is It common among females. In 2022, 2,296,840 women were diagnosed with therapy evolving through development Poly (ADP-ribose) polymerase (PARP) inhibitors, which offering people specific genetic profiles new hope research into disease continues. focuses on patients BRCA1 and BRCA2 mutations. This review summarizes recent mechanisms action PARP inhibitors their implications for therapy. We how therapeutic applications developing highlight studies showing effectiveness these medicines whether used alone or combination. Furthermore, significance customized highlighted enhancing patient outcomes we address function testing identifying candidates inhibition. Recommendations future areas to maximize potential also included, along challenges limits clinical usage. objective this improve our comprehension complex interaction between biology knowledge will help guide screening approaches, practice, support preventive initiatives at risk.

Language: Английский

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Isolation and purification of sinensetin, and in silico screening of phytochemicals from Orthosiphon aristatus (Blume) Miq. for anti-breast cancer activity

Natural Product Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: April 25, 2025

Orthosiphon aristatus (Blume) Miq. (O. aristatus), a traditional herbal medicine, exhibits wide range of biological activities but its anti-breast cancer effect is not fully established. In this study, sinensetin was isolated and purified through multistep process, beginning with liquid-liquid partitioning using dichloromethane, followed by classic column chromatography gradient elution. Preparative high-performance liquid acetonitrile-water (41:59, v/v) mobile phase successfully to 95% purity. silico screening O. aristatus-derived bioactive compounds revealed that flavonoids (including sinensetin), polyphenols (i.e. 2,3-O-dicaffeoyltartaric acid, rosmarinic acid), diterpenoid trans-ozic triterpenoids dipeptide aurantiamide acetate) have good binding affinities 17β-HSD1, ErbB2/HER2 PI3K-PKB/Akt proteins. These findings suggest arsistatus could be rich natural source inhibitors targeting molecular pathways involved in breast cancer, offering promising therapeutic benefits.

Language: Английский

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Sinensetin Inhibits Angiogenesis in Lung Adenocarcinoma via the miR-374c-5p/VEGF-A/VEGFR-2/AKT Axis

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(3), P. 2413 - 2425

Published: July 20, 2024

Language: Английский

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Sinensetin inhibits the movement ability and tumor immune microenvironment of non‐small cell lung cancer through the inactivation of AKT/β‐catenin axis

Journal of Biochemical and Molecular Toxicology, Journal Year: 2024, Volume and Issue: 38(11)

Published: Oct. 21, 2024

Although current treatment strategies have improved clinical outcomes of non-small cell lung cancer (NSCLC) patients, side effect and prognosis remain a hindrance. Thus, safer more effective therapeutical drugs are needed for NSCLC. Sinensetin (Sin) is flavonoid from citrus fruits, which exhibits antitumor on diverse cancers. However, the mechanism Sin NSCLC unknown. In this study, lines, tumor-bearing mice were treated with Sin. The addressed using counting kit-8, transwell, enzyme-linked immunosorbent assay, hematoxylin eosin, immunohistochemistry, western blot analysis assays in both animal models. reduced viability A549 H1299, IC50 81.46 µM 93.15 µM, respectively. decreased invaded numbers, expression N-cadherin vascular endothelial growth factor A (VEGFA), while increased E-cadherin level, cytotoxicity CD8

Language: Английский

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Flavonoids of Euphorbia hirta inhibit inflammatory mechanisms via Nrf2 and NF-κB pathways

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Language: Английский

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Chinese herbal medicine for the treatment of diabetic nephropathy: From clinical evidence to potential mechanisms

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118179 - 118179

Published: April 16, 2024

Language: Английский

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Flavonoids of Euphorbia hirta inhibit inflammatory mechanisms via Nrf2 and NF-κB pathways

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 26, 2024

Euphorbia hirta has anti-inflammatory effects in traditional medicine, but its mechanism not been explored at the cellular and molecular levels. To this end, main active components of 65% 95% ethanol extracts were first identified by UPLC-Q-TOF/MS. The potential targets signaling pathway through network pharmacology, followed subsequent experimental validation using lipopolysaccharide (LPS)-induced inflammation model RAW264.7 cells. results showed that flavonoids. Network pharmacology revealed 71 for anti-inflammation, with a protein-protein interaction (PPI) highlighting 8 cores targets, including IL-6, TNF, et al. Furthermore, exert anti-inflammation pathways such as Nrf2 NF-κB pathway. In vitro experiment extract (EE65) quercetin (HPG) it exerted activity inhibiting expression inflammatory genes related to pathway, whereas baicalein (HCS) suppressed promoting antioxidant apoptosis study suggest development drugs.

Language: Английский

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