Regenerative Biomaterials,
Journal Year:
2024,
Volume and Issue:
11
Published: Jan. 1, 2024
Abstract
Biofabrication
techniques
allow
for
the
construction
of
biocompatible
and
biofunctional
structures
composed
from
biomaterials,
cells
biomolecules.
Bioprinting
is
an
emerging
3D
printing
method
which
utilizes
biomaterial-based
mixtures
with
other
biological
constituents
into
printable
suspensions
known
as
bioinks.
Coupled
automated
design
protocols
based
on
different
modes
droplet
deposition,
bioprinters
are
able
to
fabricate
hydrogel-based
objects
specific
architecture
geometrical
properties,
providing
necessary
environment
that
promotes
cell
growth
directs
differentiation
towards
application-related
lineages.
For
preparation
such
bioinks,
various
water-soluble
biomaterials
have
been
employed,
including
natural
synthetic
biopolymers,
inorganic
materials.
Bioprinted
constructs
considered
be
one
most
promising
avenues
in
regenerative
medicine
due
their
native
organ
biomimicry.
a
successful
application,
bioprinted
should
meet
particular
criteria
optimal
response,
mechanical
properties
similar
target
tissue,
high
levels
reproducibility
fidelity,
but
also
increased
upscaling
capability.
In
this
review,
we
highlight
recent
advances
bioprinting,
focusing
regeneration
tissues
bone,
cartilage,
cardiovascular,
neural,
skin
organs
liver,
kidney,
pancreas
lungs.
We
discuss
rapidly
developing
co-culture
bioprinting
systems
used
resemble
complexity
crosstalk
between
populations
regeneration.
Moreover,
report
basic
physical
principles
governing
ideal
bioink
biomaterials’
potential.
examine
critically
present
status
regarding
its
applicability
current
limitations
need
overcome
establish
it
at
forefront
artificial
production
transplantation.
Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
32, P. 356 - 384
Published: Oct. 21, 2023
Three-dimensional
bioprinting
is
an
advanced
tissue
fabrication
technique
that
allows
printing
complex
structures
with
precise
positioning
of
multiple
cell
types
layer-by-layer.
Compared
to
other
methods,
extrusion
has
several
advantages
print
large-sized
constructs
and
organ
models
due
large
build
volume.
Extrusion
using
sacrificial,
support
embedded
strategies
have
been
successfully
employed
facilitate
hollow
structures.
Embedded
a
gel-in-gel
approach
developed
overcome
the
gravitational
overhanging
limits
micron-scale
resolution.
In
bioprinting,
deposition
bioinks
into
microgel
or
granular
bath
will
be
facilitated
by
sol-gel
transition
through
needle
movement
inside
medium.
This
review
outlines
various
polymers
used
in
systems
advantages,
limitations,
efficacy
vascularized
tissues
Further,
essential
requirements
like
viscoelasticity,
stability,
transparency
easy
extraction
human
scale
organs
are
discussed.
Additionally,
geometries
vascular
constructs,
heart,
bone,
octopus
jellyfish
printed
assisted
methods
their
anatomical
features
elaborated.
Finally,
challenges
clinical
translation
future
scope
these
replace
native
envisaged.
International Materials Reviews,
Journal Year:
2023,
Volume and Issue:
68(7), P. 901 - 942
Published: Jan. 27, 2023
Hyaluronic
acid
(HA)
is
of
immense
importance
to
biomaterials
science
and
biomedical
engineering.
It
finding
applications
in
diverse
areas
bioengineering
ranging
from
scaffolds
for
disease
modelling
tissue
culture
reconstruction.
This
review
focuses
on
recent
research
the
role
HA
as
a
photo-cross-linked
bioink
its
combating
bone
cartilage-related
disease,
injury
disorders.
Photo
chemical
modifications
3D
fabrication
technologies
employed
produce
HA-modified
materials
are
analysed
provide
fundamental
understanding
structure–function-property
relationships
that
influence
printability,
shape
fidelity
biological
performance
both
in-vitro
in-vivo.
The
article
concludes
with
future
vision
HA-based
bioinks
their
deployment
light-based
bioprinting
cartilage
repair.
Biofabrication,
Journal Year:
2024,
Volume and Issue:
16(2), P. 025004 - 025004
Published: Jan. 4, 2024
Abstract
Foreign
body
response
(FBR)
is
a
pervasive
problem
for
biomaterials
used
in
tissue
engineering.
Zwitterionic
hydrogels
have
emerged
as
an
effective
solution
to
this
problem,
due
their
ultra-low
fouling
properties,
which
enable
them
effectively
inhibit
FBR
vivo
.
However,
no
versatile
zwitterionic
bioink
that
allows
high
resolution
extrusion
bioprinting
of
implants
has
thus
far
been
reported.
In
work,
we
introduce
simple,
novel
method
producing
microgel
bioink,
using
alginate
methacrylate
(AlgMA)
crosslinker
and
mechanical
fragmentation
fabrication
method.
Photocrosslinked
made
carboxybetaine
acrylamide
(CBAA)
sulfobetaine
(SBMA)
are
mechanically
fragmented
through
meshes
with
aperture
diameters
50
90
µ
m
produce
bioink.
The
bioinks
both
sizes
showed
excellent
rheological
properties
were
high-resolution
printing
objects
overhanging
features
without
requiring
support
structure
or
bath.
AlgMA
dual
role,
allowing
primary
photocrosslinking
the
bulk
hydrogel
well
secondary
ionic
crosslinking
produced
microgels,
quickly
stabilize
printed
construct
calcium
bath
microporous
scaffold.
Scaffolds
∼20%
porosity,
they
supported
viability
chondrogenesis
encapsulated
human
chondrocytes.
Finally,
meniscus
model
was
bioprinted,
demonstrate
bioink’s
versatility
at
large,
cell-laden
constructs
stable
further
vitro
culture
promote
cartilaginous
production.
This
easy
scalable
strategy
direct
cell
encapsulation
scaffold
potential
biocompatibility
nature
Regenerative Biomaterials,
Journal Year:
2024,
Volume and Issue:
11
Published: Jan. 1, 2024
Abstract
Biofabrication
techniques
allow
for
the
construction
of
biocompatible
and
biofunctional
structures
composed
from
biomaterials,
cells
biomolecules.
Bioprinting
is
an
emerging
3D
printing
method
which
utilizes
biomaterial-based
mixtures
with
other
biological
constituents
into
printable
suspensions
known
as
bioinks.
Coupled
automated
design
protocols
based
on
different
modes
droplet
deposition,
bioprinters
are
able
to
fabricate
hydrogel-based
objects
specific
architecture
geometrical
properties,
providing
necessary
environment
that
promotes
cell
growth
directs
differentiation
towards
application-related
lineages.
For
preparation
such
bioinks,
various
water-soluble
biomaterials
have
been
employed,
including
natural
synthetic
biopolymers,
inorganic
materials.
Bioprinted
constructs
considered
be
one
most
promising
avenues
in
regenerative
medicine
due
their
native
organ
biomimicry.
a
successful
application,
bioprinted
should
meet
particular
criteria
optimal
response,
mechanical
properties
similar
target
tissue,
high
levels
reproducibility
fidelity,
but
also
increased
upscaling
capability.
In
this
review,
we
highlight
recent
advances
bioprinting,
focusing
regeneration
tissues
bone,
cartilage,
cardiovascular,
neural,
skin
organs
liver,
kidney,
pancreas
lungs.
We
discuss
rapidly
developing
co-culture
bioprinting
systems
used
resemble
complexity
crosstalk
between
populations
regeneration.
Moreover,
report
basic
physical
principles
governing
ideal
bioink
biomaterials’
potential.
examine
critically
present
status
regarding
its
applicability
current
limitations
need
overcome
establish
it
at
forefront
artificial
production
transplantation.
