Pancreatic Cancer Cell-derived Migrasomes Promote Cancer Progression by Fostering an Immunosuppressive Tumor Microenvironment

Cancer Letters, Journal Year: 2024, Volume and Issue: 605, P. 217289 - 217289

Published: Oct. 9, 2024

Language: Английский

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RNA binding protein Pumilio2 promotes chemoresistance of pancreatic cancer via focal adhesion pathway and interacting with transcription factor EGR1

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: Feb. 17, 2025

Pancreatic cancer (PCa) has insidious onset, high malignancy and poor prognosis. Gemcitabine (GEM) is one of the first-line chemotherapy drugs for PCa. However, GEM resistance always been a bottleneck problem leading to recurrence death PCa patients. RNA-binding proteins (RBPs) are important that regulate transportation, splicing, stability translation RNA. Abnormal expression RBPs often lead series abnormal accumulation or degradation downstream RNA resulting in various diseases. In our study, we utilized RIP seq, RIP-qPCR, vitro vivo experiments found pumilio2 (PUM2) was PCa, promoted by regulating mRNA integrin Alpha 3 (ITGA3) other genes focal adhesion pathway, there positive feedback regulation between PUM2 transcription factor early growth response gene 1 (EGR1), binding 3′UTR region EGR1 mRNA, promoter gene. The discovery EGR1/PUM2/ITGA3 axis provided solid experimental basis selection regiments patients exploration combined regimens reverse future.

Language: Английский

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Pentose Phosphate Recycling driven by Gli1 contributes to chemotherapy resistance in Cancer Cells

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217633 - 217633

Published: March 1, 2025

Language: Английский

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Broussoflavonol B induces S-phase arrest and apoptosis in pancreatic cancer cells by modulating the cell cycle checkpoint through inhibition of the AURKA/PLK1 pathway

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 17, 2025

Broussoflavonol B (Bf-B), a flavonoid compound identified in the roots of Daphne giraldii Nitsche, has been extensively investigated for its potential anti-inflammatory, antioxidant, and anticancer properties. However, precise mechanism underlying regulation AURKA/PLK1 pathway-mediated cell cycle arrest by Bf-B pancreatic cancer remains poorly understood. The objective this study was to investigate inhibitory effect on ductal adenocarcinoma (PDAC) mechanism. A CCK8 assay conducted identify flavonoids with highest activity against PANC-1, line among 25 flavonoids. Through bioinformatics analysis molecular docking, pathogenic targets flavonoid-related were explored, key signaling pathways drug intervention analyzed. viability migration ability cells assessed following treatment via CCK8, colony formation, wound healing assays. distribution apoptosis analyzed through flow cytometry Hoechst staining. Western blotting qPCR employed expression relevant proteins genes. For vivo experiments, we xenograft mouse model evaluate efficacy Bf-B. Immunohistochemistry immunofluorescence assays proteins. In study, structure‒activity relationships evaluated. results demonstrated that diisopentenyl potent cytotoxic effects PANC-1 cells. AURKA, PLK1, MET might serve as inhibition disease progression PDAC patients. inhibits proliferation BXPC-3 induces S-phase arrest, apoptosis, DNA damage. Moreover, western blot experiments indicated exerts downregulating genes encoding AURKA/PLK1, checkpoint kinase ATR/CHK1/CDC25C, Cyclin B1/CDK1 pathway-related upregulating PP53, P21, histone H2A. XS139ph expression. xenograft-bearing mice, reduced dose-dependent manner, accompanied an increase be therapeutic agent because suppress AURKA/PLK1.

Language: Английский

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Biodistribution and therapeutic efficacy of a gold nanoparticle-based targeted drug delivery system against pancreatic cancer

Cancer Letters, Journal Year: 2024, Volume and Issue: 589, P. 216810 - 216810

Published: March 15, 2024

Language: Английский

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Evaluation of a new scoring system for assessing nerve invasion in resected pancreatic cancer: a single-center retrospective analysis

Cancer Letters, Journal Year: 2024, Volume and Issue: 603, P. 217213 - 217213

Published: Sept. 6, 2024

Language: Английский

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SIRT7 knockdown promotes gemcitabine sensitivity of pancreatic cancer cell via upregulation of GLUT3 expression

Cancer Letters, Journal Year: 2024, Volume and Issue: 598, P. 217109 - 217109

Published: July 14, 2024

Language: Английский

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Embedded bioprinted multicellular spheroids modeling pancreatic cancer bioarchitecture towards advanced drug therapy

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(7), P. 1788 - 1797

Published: Jan. 1, 2024

A facile embedded dot bioprinting system for bioengineering desmoplastic PDAC spheroids with scalable, flexible and robust performance, or multi-type spheroid patterns advanced drug therapy disease mechanism exploration, is introduced.

Language: Английский

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Targeting STAT3 potentiates CDK4/6 inhibitors therapy in head and neck squamous cell carcinoma

Cancer Letters, Journal Year: 2024, Volume and Issue: 593, P. 216956 - 216956

Published: May 11, 2024

Language: Английский

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Secretory Trefoil Factor 1 (TFF1) promotes gemcitabine resistance through chemokine receptor CXCR4 in Pancreatic Ductal Adenocarcinoma

Cancer Letters, Journal Year: 2024, Volume and Issue: 598, P. 217097 - 217097

Published: July 2, 2024

Language: Английский

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